Cargando…

Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids

Little is known about the ability for epithelial regeneration and wound healing in patients with inflammatory bowel diseases. We evaluated the epithelial proliferation and wound healing ability of patients with Crohn’s disease (CD) using patient-derived intestinal organoids. Human intestinal organoi...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Chansu, Hong, Sung-Noh, Kim, Eun-Ran, Chang, Dong-Kyung, Kim, Young-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199630/
https://www.ncbi.nlm.nih.gov/pubmed/34199463
http://dx.doi.org/10.3390/ijms22116013
_version_ 1783707421634461696
author Lee, Chansu
Hong, Sung-Noh
Kim, Eun-Ran
Chang, Dong-Kyung
Kim, Young-Ho
author_facet Lee, Chansu
Hong, Sung-Noh
Kim, Eun-Ran
Chang, Dong-Kyung
Kim, Young-Ho
author_sort Lee, Chansu
collection PubMed
description Little is known about the ability for epithelial regeneration and wound healing in patients with inflammatory bowel diseases. We evaluated the epithelial proliferation and wound healing ability of patients with Crohn’s disease (CD) using patient-derived intestinal organoids. Human intestinal organoids were constructed in a three-dimensional intestinal crypt culture of enteroscopic biopsy samples from controls and CD patients. The organoid-forming efficiency of ileal crypts derived from CD patients was reduced compared with those from control subjects (p < 0.001). Long-term cultured organoids (≥6 passages) derived from controls and CD patients showed an indistinguishable microscopic appearance and culturing behavior. Under TNFα-enriched conditions (30 ng/mL), the organoid reconstitution rate and cell viability of CD patient-derived organoids were significantly lower than those of the control organoids (p < 0.05 for each). The number of EdU+ cells was significantly lower in TNFα-treated organoids derived from CD patients than in TNFα-treated control organoids (p < 0.05). In a wound healing assay, the unhealed area in TNFα-treated CD patient-derived organoids was significantly larger than that of TNFα-treated control organoids (p < 0.001). The wound healing ability of CD patient-derived organoids is reduced in TNFα-enriched conditions, due to reduced cell proliferation. Epithelial regeneration ability may be impaired in patients with CD.
format Online
Article
Text
id pubmed-8199630
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-81996302021-06-14 Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids Lee, Chansu Hong, Sung-Noh Kim, Eun-Ran Chang, Dong-Kyung Kim, Young-Ho Int J Mol Sci Article Little is known about the ability for epithelial regeneration and wound healing in patients with inflammatory bowel diseases. We evaluated the epithelial proliferation and wound healing ability of patients with Crohn’s disease (CD) using patient-derived intestinal organoids. Human intestinal organoids were constructed in a three-dimensional intestinal crypt culture of enteroscopic biopsy samples from controls and CD patients. The organoid-forming efficiency of ileal crypts derived from CD patients was reduced compared with those from control subjects (p < 0.001). Long-term cultured organoids (≥6 passages) derived from controls and CD patients showed an indistinguishable microscopic appearance and culturing behavior. Under TNFα-enriched conditions (30 ng/mL), the organoid reconstitution rate and cell viability of CD patient-derived organoids were significantly lower than those of the control organoids (p < 0.05 for each). The number of EdU+ cells was significantly lower in TNFα-treated organoids derived from CD patients than in TNFα-treated control organoids (p < 0.05). In a wound healing assay, the unhealed area in TNFα-treated CD patient-derived organoids was significantly larger than that of TNFα-treated control organoids (p < 0.001). The wound healing ability of CD patient-derived organoids is reduced in TNFα-enriched conditions, due to reduced cell proliferation. Epithelial regeneration ability may be impaired in patients with CD. MDPI 2021-06-02 /pmc/articles/PMC8199630/ /pubmed/34199463 http://dx.doi.org/10.3390/ijms22116013 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Chansu
Hong, Sung-Noh
Kim, Eun-Ran
Chang, Dong-Kyung
Kim, Young-Ho
Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids
title Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids
title_full Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids
title_fullStr Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids
title_full_unstemmed Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids
title_short Epithelial Regeneration Ability of Crohn’s Disease Assessed Using Patient-Derived Intestinal Organoids
title_sort epithelial regeneration ability of crohn’s disease assessed using patient-derived intestinal organoids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8199630/
https://www.ncbi.nlm.nih.gov/pubmed/34199463
http://dx.doi.org/10.3390/ijms22116013
work_keys_str_mv AT leechansu epithelialregenerationabilityofcrohnsdiseaseassessedusingpatientderivedintestinalorganoids
AT hongsungnoh epithelialregenerationabilityofcrohnsdiseaseassessedusingpatientderivedintestinalorganoids
AT kimeunran epithelialregenerationabilityofcrohnsdiseaseassessedusingpatientderivedintestinalorganoids
AT changdongkyung epithelialregenerationabilityofcrohnsdiseaseassessedusingpatientderivedintestinalorganoids
AT kimyoungho epithelialregenerationabilityofcrohnsdiseaseassessedusingpatientderivedintestinalorganoids