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Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli
Single mutations can confer resistance to antibiotics. Identifying such mutations can help to develop and improve drugs. Here, we systematically screen for candidate quinolone resistance-conferring mutations. We sequenced highly diverse wastewater E. coli and performed a genome-wide association stud...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200043/ https://www.ncbi.nlm.nih.gov/pubmed/34199768 http://dx.doi.org/10.3390/ijms22116063 |
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author | Malekian, Negin Al-Fatlawi, Ali Berendonk, Thomas U. Schroeder, Michael |
author_facet | Malekian, Negin Al-Fatlawi, Ali Berendonk, Thomas U. Schroeder, Michael |
author_sort | Malekian, Negin |
collection | PubMed |
description | Single mutations can confer resistance to antibiotics. Identifying such mutations can help to develop and improve drugs. Here, we systematically screen for candidate quinolone resistance-conferring mutations. We sequenced highly diverse wastewater E. coli and performed a genome-wide association study (GWAS) to determine associations between over 200,000 mutations and quinolone resistance phenotypes. We uncovered 13 statistically significant mutations including 1 located at the active site of the biofilm dispersal gene bdcA and 6 silent mutations in the aminoacyl-tRNA synthetase valS. The study also recovered the known mutations in the topoisomerases gyrase (gyrA) and topoisomerase IV (parC). In summary, we demonstrate that GWAS effectively and comprehensively identifies resistance mutations without a priori knowledge of targets and mode of action. The results suggest that mutations in the bdcA and valS genes, which are involved in biofilm dispersal and translation, may lead to novel resistance mechanisms. |
format | Online Article Text |
id | pubmed-8200043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82000432021-06-14 Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli Malekian, Negin Al-Fatlawi, Ali Berendonk, Thomas U. Schroeder, Michael Int J Mol Sci Article Single mutations can confer resistance to antibiotics. Identifying such mutations can help to develop and improve drugs. Here, we systematically screen for candidate quinolone resistance-conferring mutations. We sequenced highly diverse wastewater E. coli and performed a genome-wide association study (GWAS) to determine associations between over 200,000 mutations and quinolone resistance phenotypes. We uncovered 13 statistically significant mutations including 1 located at the active site of the biofilm dispersal gene bdcA and 6 silent mutations in the aminoacyl-tRNA synthetase valS. The study also recovered the known mutations in the topoisomerases gyrase (gyrA) and topoisomerase IV (parC). In summary, we demonstrate that GWAS effectively and comprehensively identifies resistance mutations without a priori knowledge of targets and mode of action. The results suggest that mutations in the bdcA and valS genes, which are involved in biofilm dispersal and translation, may lead to novel resistance mechanisms. MDPI 2021-06-04 /pmc/articles/PMC8200043/ /pubmed/34199768 http://dx.doi.org/10.3390/ijms22116063 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Malekian, Negin Al-Fatlawi, Ali Berendonk, Thomas U. Schroeder, Michael Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli |
title | Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli |
title_full | Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli |
title_fullStr | Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli |
title_full_unstemmed | Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli |
title_short | Mutations in bdcA and valS Correlate with Quinolone Resistance in Wastewater Escherichia coli |
title_sort | mutations in bdca and vals correlate with quinolone resistance in wastewater escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200043/ https://www.ncbi.nlm.nih.gov/pubmed/34199768 http://dx.doi.org/10.3390/ijms22116063 |
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