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Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle

BACKGROUND: In vivo muscle protein synthesis rates are typically assessed by measuring the incorporation rate of stable isotope labelled amino acids in skeletal muscle tissue collected from vastus lateralis muscle. It remains to be established whether muscle protein synthesis rates in the vastus lat...

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Autores principales: Smeets, Joey S.J., Horstman, Astrid M.H., van Dijk, David P.J., van Boxtel, Astrid G.M., ter Woorst, Joost F., Damink, Steven W.M. Olde, Schijns, Olaf E.M.G., van Loon, Luc J.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200451/
https://www.ncbi.nlm.nih.gov/pubmed/33951313
http://dx.doi.org/10.1002/jcsm.12701
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author Smeets, Joey S.J.
Horstman, Astrid M.H.
van Dijk, David P.J.
van Boxtel, Astrid G.M.
ter Woorst, Joost F.
Damink, Steven W.M. Olde
Schijns, Olaf E.M.G.
van Loon, Luc J.C.
author_facet Smeets, Joey S.J.
Horstman, Astrid M.H.
van Dijk, David P.J.
van Boxtel, Astrid G.M.
ter Woorst, Joost F.
Damink, Steven W.M. Olde
Schijns, Olaf E.M.G.
van Loon, Luc J.C.
author_sort Smeets, Joey S.J.
collection PubMed
description BACKGROUND: In vivo muscle protein synthesis rates are typically assessed by measuring the incorporation rate of stable isotope labelled amino acids in skeletal muscle tissue collected from vastus lateralis muscle. It remains to be established whether muscle protein synthesis rates in the vastus lateralis are representative of muscle protein synthesis rates of other muscle groups. We hypothesized that post‐absorptive muscle protein synthesis rates differ between vastus lateralis and rectus abdominis, pectoralis major, or temporalis muscle in vivo in humans. METHODS: Twenty‐four patients (62 ± 3 years, 42% female), scheduled to undergo surgery, participated in this study and underwent primed continuous intravenous infusions with l‐[ring‐(13)C(6)]‐phenylalanine. During the surgical procedures, serum samples were collected, and muscle tissue was obtained from the vastus lateralis as well as from the rectus abdominis, pectoralis major, or temporalis muscle. Fractional mixed muscle protein synthesis rates (%/h) were assessed by measuring the incorporation of l‐[ring‐(13)C(6)]‐phenylalanine into muscle tissue protein. RESULTS: Serum l‐[ring‐(13)C(6)]‐phenylalanine enrichments did not change throughout the infusion period. Post‐absorptive muscle protein synthesis rates calculated based upon serum l‐[ring‐(13)C(6)]‐phenylalanine enrichments did not differ between vastus lateralis and rectus abdominis (0.032 ± 0.004 vs. 0.038 ± 0.003%/h), vastus lateralis and pectoralis major, (0.025 ± 0.003 vs. 0.022 ± 0.005%/h) or vastus lateralis and temporalis (0.047 ± 0.005 vs. 0.043 ± 0.005%/h) muscle, respectively (P > 0.05). When fractional muscle protein synthesis rates were calculated based upon tissue‐free l‐[ring‐(13)C(6)]‐phenylalanine enrichments as the preferred precursor pool, muscle protein synthesis rates were significantly higher in rectus abdominis (0.089 ± 0.008%/h) compared with vastus lateralis (0.054 ± 0.005%/h) muscle (P < 0.01). No differences were observed between fractional muscle protein synthesis rates in vastus lateralis and pectoralis major (0.046 ± 0.003 vs. 0.041 ± 0.008%/h) or vastus lateralis and temporalis (0.073 ± 0.008 vs. 0.083 ± 0.011%/h) muscle, respectively. CONCLUSIONS: Post‐absorptive muscle protein synthesis rates are higher in rectus abdominis when compared with vastus lateralis muscle. Post‐absorptive muscle protein synthesis rates do not differ between vastus lateralis and pectoralis major or temporalis muscle. Protein synthesis rates in muscle tissue samples obtained during surgery do not necessarily represent a good proxy for appendicular skeletal muscle protein synthesis rates.
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spelling pubmed-82004512021-06-15 Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle Smeets, Joey S.J. Horstman, Astrid M.H. van Dijk, David P.J. van Boxtel, Astrid G.M. ter Woorst, Joost F. Damink, Steven W.M. Olde Schijns, Olaf E.M.G. van Loon, Luc J.C. J Cachexia Sarcopenia Muscle Original Articles BACKGROUND: In vivo muscle protein synthesis rates are typically assessed by measuring the incorporation rate of stable isotope labelled amino acids in skeletal muscle tissue collected from vastus lateralis muscle. It remains to be established whether muscle protein synthesis rates in the vastus lateralis are representative of muscle protein synthesis rates of other muscle groups. We hypothesized that post‐absorptive muscle protein synthesis rates differ between vastus lateralis and rectus abdominis, pectoralis major, or temporalis muscle in vivo in humans. METHODS: Twenty‐four patients (62 ± 3 years, 42% female), scheduled to undergo surgery, participated in this study and underwent primed continuous intravenous infusions with l‐[ring‐(13)C(6)]‐phenylalanine. During the surgical procedures, serum samples were collected, and muscle tissue was obtained from the vastus lateralis as well as from the rectus abdominis, pectoralis major, or temporalis muscle. Fractional mixed muscle protein synthesis rates (%/h) were assessed by measuring the incorporation of l‐[ring‐(13)C(6)]‐phenylalanine into muscle tissue protein. RESULTS: Serum l‐[ring‐(13)C(6)]‐phenylalanine enrichments did not change throughout the infusion period. Post‐absorptive muscle protein synthesis rates calculated based upon serum l‐[ring‐(13)C(6)]‐phenylalanine enrichments did not differ between vastus lateralis and rectus abdominis (0.032 ± 0.004 vs. 0.038 ± 0.003%/h), vastus lateralis and pectoralis major, (0.025 ± 0.003 vs. 0.022 ± 0.005%/h) or vastus lateralis and temporalis (0.047 ± 0.005 vs. 0.043 ± 0.005%/h) muscle, respectively (P > 0.05). When fractional muscle protein synthesis rates were calculated based upon tissue‐free l‐[ring‐(13)C(6)]‐phenylalanine enrichments as the preferred precursor pool, muscle protein synthesis rates were significantly higher in rectus abdominis (0.089 ± 0.008%/h) compared with vastus lateralis (0.054 ± 0.005%/h) muscle (P < 0.01). No differences were observed between fractional muscle protein synthesis rates in vastus lateralis and pectoralis major (0.046 ± 0.003 vs. 0.041 ± 0.008%/h) or vastus lateralis and temporalis (0.073 ± 0.008 vs. 0.083 ± 0.011%/h) muscle, respectively. CONCLUSIONS: Post‐absorptive muscle protein synthesis rates are higher in rectus abdominis when compared with vastus lateralis muscle. Post‐absorptive muscle protein synthesis rates do not differ between vastus lateralis and pectoralis major or temporalis muscle. Protein synthesis rates in muscle tissue samples obtained during surgery do not necessarily represent a good proxy for appendicular skeletal muscle protein synthesis rates. John Wiley and Sons Inc. 2021-05-05 2021-06 /pmc/articles/PMC8200451/ /pubmed/33951313 http://dx.doi.org/10.1002/jcsm.12701 Text en © 2021 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Smeets, Joey S.J.
Horstman, Astrid M.H.
van Dijk, David P.J.
van Boxtel, Astrid G.M.
ter Woorst, Joost F.
Damink, Steven W.M. Olde
Schijns, Olaf E.M.G.
van Loon, Luc J.C.
Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
title Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
title_full Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
title_fullStr Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
title_full_unstemmed Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
title_short Basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
title_sort basal protein synthesis rates differ between vastus lateralis and rectus abdominis muscle
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8200451/
https://www.ncbi.nlm.nih.gov/pubmed/33951313
http://dx.doi.org/10.1002/jcsm.12701
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