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Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy
Vitreoretinal lymphoma (VRL) is an uncommon eye malignancy, and VRLs of T cell origin are rare. They are difficult to treat, and their molecular underpinnings, including actionable genomic alterations, remain to be elucidated. At present, vitreous fluid liquid biopsies represent a valuable VRL sampl...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201094/ https://www.ncbi.nlm.nih.gov/pubmed/34198843 http://dx.doi.org/10.3390/ijms22116099 |
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author | Cani, Andi K. Toral, Marcus A. Balikov, Daniel A. Betz, Bryan L. Hu, Kevin Liu, Chia-Jen Prifti, Matthew V. Chinnaiyan, Arul M. Tomlins, Scott A. Mahajan, Vinit B. Rao, Rajesh C. |
author_facet | Cani, Andi K. Toral, Marcus A. Balikov, Daniel A. Betz, Bryan L. Hu, Kevin Liu, Chia-Jen Prifti, Matthew V. Chinnaiyan, Arul M. Tomlins, Scott A. Mahajan, Vinit B. Rao, Rajesh C. |
author_sort | Cani, Andi K. |
collection | PubMed |
description | Vitreoretinal lymphoma (VRL) is an uncommon eye malignancy, and VRLs of T cell origin are rare. They are difficult to treat, and their molecular underpinnings, including actionable genomic alterations, remain to be elucidated. At present, vitreous fluid liquid biopsies represent a valuable VRL sample for molecular analysis to study VRLs. In this study, we report the molecular diagnostic workup of a rare case of bilateral T cell VRL and characterize its genomic landscape, including identification of potentially targetable alterations. Using next-generation sequencing of vitreous-derived DNA with a pan-cancer 126-gene panel, we found a copy number gain of BRAF and copy number loss of tumor suppressor DNMT3A. To the best of our knowledge, this represents the first exploration of the T cell VRL cancer genome and supports vitreous liquid biopsy as a suitable approach for precision oncology treatments. |
format | Online Article Text |
id | pubmed-8201094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82010942021-06-15 Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy Cani, Andi K. Toral, Marcus A. Balikov, Daniel A. Betz, Bryan L. Hu, Kevin Liu, Chia-Jen Prifti, Matthew V. Chinnaiyan, Arul M. Tomlins, Scott A. Mahajan, Vinit B. Rao, Rajesh C. Int J Mol Sci Case Report Vitreoretinal lymphoma (VRL) is an uncommon eye malignancy, and VRLs of T cell origin are rare. They are difficult to treat, and their molecular underpinnings, including actionable genomic alterations, remain to be elucidated. At present, vitreous fluid liquid biopsies represent a valuable VRL sample for molecular analysis to study VRLs. In this study, we report the molecular diagnostic workup of a rare case of bilateral T cell VRL and characterize its genomic landscape, including identification of potentially targetable alterations. Using next-generation sequencing of vitreous-derived DNA with a pan-cancer 126-gene panel, we found a copy number gain of BRAF and copy number loss of tumor suppressor DNMT3A. To the best of our knowledge, this represents the first exploration of the T cell VRL cancer genome and supports vitreous liquid biopsy as a suitable approach for precision oncology treatments. MDPI 2021-06-05 /pmc/articles/PMC8201094/ /pubmed/34198843 http://dx.doi.org/10.3390/ijms22116099 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Case Report Cani, Andi K. Toral, Marcus A. Balikov, Daniel A. Betz, Bryan L. Hu, Kevin Liu, Chia-Jen Prifti, Matthew V. Chinnaiyan, Arul M. Tomlins, Scott A. Mahajan, Vinit B. Rao, Rajesh C. Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy |
title | Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy |
title_full | Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy |
title_fullStr | Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy |
title_full_unstemmed | Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy |
title_short | Molecular Characterization of a Rare Case of Bilateral Vitreoretinal T Cell Lymphoma through Vitreous Liquid Biopsy |
title_sort | molecular characterization of a rare case of bilateral vitreoretinal t cell lymphoma through vitreous liquid biopsy |
topic | Case Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201094/ https://www.ncbi.nlm.nih.gov/pubmed/34198843 http://dx.doi.org/10.3390/ijms22116099 |
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