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Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.)
Drought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber s...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201130/ https://www.ncbi.nlm.nih.gov/pubmed/34200118 http://dx.doi.org/10.3390/ijms22116123 |
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author | Schumacher, Christina Thümecke, Susanne Schilling, Florian Köhl, Karin Kopka, Joachim Sprenger, Heike Hincha, Dirk Karl Walther, Dirk Seddig, Sylvia Peters, Rolf Zuther, Ellen Haas, Manuela Horn, Renate |
author_facet | Schumacher, Christina Thümecke, Susanne Schilling, Florian Köhl, Karin Kopka, Joachim Sprenger, Heike Hincha, Dirk Karl Walther, Dirk Seddig, Sylvia Peters, Rolf Zuther, Ellen Haas, Manuela Horn, Renate |
author_sort | Schumacher, Christina |
collection | PubMed |
description | Drought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber starch content, tuber starch yield, tuber fresh weight, selected transcripts and metabolites under control and drought stress conditions. Eight genomic regions of major interest for drought tolerance were identified, three representing standalone DRYM QTL. Candidate genes, e.g., from signaling pathways for ethylene, abscisic acid and brassinosteroids, and genes encoding cell wall remodeling enzymes were identified within DRYM QTL. Co-localizations of DRYM QTL and QTL for tuber starch content, tuber starch yield and tuber fresh weight with underlying genes of the carbohydrate metabolism were observed. Overlaps of DRYM QTL with metabolite QTL for ribitol or galactinol may indicate trade-offs between starch and compatible solute biosynthesis. Expression QTL confirmed the drought stress relevance of selected transcripts by overlaps with DRYM QTL. Bulked segregant analyses combined with next-generation sequencing (BSAseq) were used to identify mutations in genes under the DRYM QTL on linkage group 3. Future analyses of identified genes for drought tolerance will give a better insight into drought tolerance in potatoes. |
format | Online Article Text |
id | pubmed-8201130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82011302021-06-15 Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.) Schumacher, Christina Thümecke, Susanne Schilling, Florian Köhl, Karin Kopka, Joachim Sprenger, Heike Hincha, Dirk Karl Walther, Dirk Seddig, Sylvia Peters, Rolf Zuther, Ellen Haas, Manuela Horn, Renate Int J Mol Sci Article Drought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber starch content, tuber starch yield, tuber fresh weight, selected transcripts and metabolites under control and drought stress conditions. Eight genomic regions of major interest for drought tolerance were identified, three representing standalone DRYM QTL. Candidate genes, e.g., from signaling pathways for ethylene, abscisic acid and brassinosteroids, and genes encoding cell wall remodeling enzymes were identified within DRYM QTL. Co-localizations of DRYM QTL and QTL for tuber starch content, tuber starch yield and tuber fresh weight with underlying genes of the carbohydrate metabolism were observed. Overlaps of DRYM QTL with metabolite QTL for ribitol or galactinol may indicate trade-offs between starch and compatible solute biosynthesis. Expression QTL confirmed the drought stress relevance of selected transcripts by overlaps with DRYM QTL. Bulked segregant analyses combined with next-generation sequencing (BSAseq) were used to identify mutations in genes under the DRYM QTL on linkage group 3. Future analyses of identified genes for drought tolerance will give a better insight into drought tolerance in potatoes. MDPI 2021-06-07 /pmc/articles/PMC8201130/ /pubmed/34200118 http://dx.doi.org/10.3390/ijms22116123 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schumacher, Christina Thümecke, Susanne Schilling, Florian Köhl, Karin Kopka, Joachim Sprenger, Heike Hincha, Dirk Karl Walther, Dirk Seddig, Sylvia Peters, Rolf Zuther, Ellen Haas, Manuela Horn, Renate Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.) |
title | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.) |
title_full | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.) |
title_fullStr | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.) |
title_full_unstemmed | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.) |
title_short | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (Solanum tuberosum L.) |
title_sort | genome-wide approach to identify quantitative trait loci for drought tolerance in tetraploid potato (solanum tuberosum l.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201130/ https://www.ncbi.nlm.nih.gov/pubmed/34200118 http://dx.doi.org/10.3390/ijms22116123 |
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