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Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink

Gelatin is a natural biopolymer extensively used for tissue engineering applications due to its similarities to the native extracellular matrix. However, the rheological properties of gelatin formulations are not ideal for extrusion-based bioprinting. In this work, we present an approach to improve...

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Autores principales: Lapomarda, Anna, Pulidori, Elena, Cerqueni, Giorgia, Chiesa, Irene, De Blasi, Matteo, Geven, Mike Alexander, Montemurro, Francesca, Duce, Celia, Mattioli-Belmonte, Monica, Tiné, Maria Rosaria, Vozzi, Giovanni, De Maria, Carmelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201283/
https://www.ncbi.nlm.nih.gov/pubmed/34198912
http://dx.doi.org/10.3390/ma14113109
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author Lapomarda, Anna
Pulidori, Elena
Cerqueni, Giorgia
Chiesa, Irene
De Blasi, Matteo
Geven, Mike Alexander
Montemurro, Francesca
Duce, Celia
Mattioli-Belmonte, Monica
Tiné, Maria Rosaria
Vozzi, Giovanni
De Maria, Carmelo
author_facet Lapomarda, Anna
Pulidori, Elena
Cerqueni, Giorgia
Chiesa, Irene
De Blasi, Matteo
Geven, Mike Alexander
Montemurro, Francesca
Duce, Celia
Mattioli-Belmonte, Monica
Tiné, Maria Rosaria
Vozzi, Giovanni
De Maria, Carmelo
author_sort Lapomarda, Anna
collection PubMed
description Gelatin is a natural biopolymer extensively used for tissue engineering applications due to its similarities to the native extracellular matrix. However, the rheological properties of gelatin formulations are not ideal for extrusion-based bioprinting. In this work, we present an approach to improve gelatin bioprinting performances by using pectin as a rheology modifier of gelatin and (3-glycidyloxypropyl)trimethoxysilane (GPTMS) as a gelatin–pectin crosslinking agent. The preparation of gelatin–pectin formulations is initially optimized to obtain homogenous gelatin–pectin gels. Since the use of GPTMS requires a drying step to induce the completion of the crosslinking reaction, microporous gelatin–pectin–GPTMS sponges are produced through freeze-drying, and the intrinsic properties of gelatin–pectin–GPTMS networks (e.g., porosity, pore size, degree of swelling, compressive modulus, and cell adhesion) are investigated. Subsequently, rheological investigations together with bioprinting assessments demonstrate the key role of pectin in increasing the viscosity and the yield stress of low viscous gelatin solutions. Water stable, three-dimensional, and self-supporting gelatin–pectin–GPTMS scaffolds with interconnected micro- and macroporosity are successfully obtained by combining extrusion-based bioprinting and freeze-drying. The proposed biofabrication approach does not require any additional temperature controller to further modulate the rheological properties of gelatin solutions and it could furthermore be extended to improve the bioprintability of other biopolymers.
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spelling pubmed-82012832021-06-15 Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink Lapomarda, Anna Pulidori, Elena Cerqueni, Giorgia Chiesa, Irene De Blasi, Matteo Geven, Mike Alexander Montemurro, Francesca Duce, Celia Mattioli-Belmonte, Monica Tiné, Maria Rosaria Vozzi, Giovanni De Maria, Carmelo Materials (Basel) Article Gelatin is a natural biopolymer extensively used for tissue engineering applications due to its similarities to the native extracellular matrix. However, the rheological properties of gelatin formulations are not ideal for extrusion-based bioprinting. In this work, we present an approach to improve gelatin bioprinting performances by using pectin as a rheology modifier of gelatin and (3-glycidyloxypropyl)trimethoxysilane (GPTMS) as a gelatin–pectin crosslinking agent. The preparation of gelatin–pectin formulations is initially optimized to obtain homogenous gelatin–pectin gels. Since the use of GPTMS requires a drying step to induce the completion of the crosslinking reaction, microporous gelatin–pectin–GPTMS sponges are produced through freeze-drying, and the intrinsic properties of gelatin–pectin–GPTMS networks (e.g., porosity, pore size, degree of swelling, compressive modulus, and cell adhesion) are investigated. Subsequently, rheological investigations together with bioprinting assessments demonstrate the key role of pectin in increasing the viscosity and the yield stress of low viscous gelatin solutions. Water stable, three-dimensional, and self-supporting gelatin–pectin–GPTMS scaffolds with interconnected micro- and macroporosity are successfully obtained by combining extrusion-based bioprinting and freeze-drying. The proposed biofabrication approach does not require any additional temperature controller to further modulate the rheological properties of gelatin solutions and it could furthermore be extended to improve the bioprintability of other biopolymers. MDPI 2021-06-05 /pmc/articles/PMC8201283/ /pubmed/34198912 http://dx.doi.org/10.3390/ma14113109 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lapomarda, Anna
Pulidori, Elena
Cerqueni, Giorgia
Chiesa, Irene
De Blasi, Matteo
Geven, Mike Alexander
Montemurro, Francesca
Duce, Celia
Mattioli-Belmonte, Monica
Tiné, Maria Rosaria
Vozzi, Giovanni
De Maria, Carmelo
Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink
title Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink
title_full Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink
title_fullStr Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink
title_full_unstemmed Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink
title_short Pectin as Rheology Modifier of a Gelatin-Based Biomaterial Ink
title_sort pectin as rheology modifier of a gelatin-based biomaterial ink
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201283/
https://www.ncbi.nlm.nih.gov/pubmed/34198912
http://dx.doi.org/10.3390/ma14113109
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