Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds

The photophysical and biological properties of two new phenanthroline-based ligand ruthenium complexes were investigated in detail. Their DNA interaction modes were determined to be the intercalation mode using spectra titration and viscosity measurements. Under irradiation, obvious photo-reduced DN...

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Autores principales: Hu, Xia, Liu, Ning-Yi, Deng, Yuan-Qing, Wang, Shan, Liu, Ting, Liu, Xue-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201372/
https://www.ncbi.nlm.nih.gov/pubmed/34200469
http://dx.doi.org/10.3390/molecules26113471
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author Hu, Xia
Liu, Ning-Yi
Deng, Yuan-Qing
Wang, Shan
Liu, Ting
Liu, Xue-Wen
author_facet Hu, Xia
Liu, Ning-Yi
Deng, Yuan-Qing
Wang, Shan
Liu, Ting
Liu, Xue-Wen
author_sort Hu, Xia
collection PubMed
description The photophysical and biological properties of two new phenanthroline-based ligand ruthenium complexes were investigated in detail. Their DNA interaction modes were determined to be the intercalation mode using spectra titration and viscosity measurements. Under irradiation, obvious photo-reduced DNA cleavages were observed in the two complexes via singlet oxygen generation. Furthermore, complex 2 showed higher DNA affinity, photocleavage activity, and singlet oxygen quantum yields than complex 1. The two complexes showed no toxicity towards tumor cells (HeLa, A549, and A375) in the dark. However, obvious photocytotoxicities were observed in the two complexes. Complex 2 exhibited large PIs (phototherapeutic indices) (ca. 400) towards HeLa cells. The study suggests that these complexes may act as DNA intercalators, DNA photocleavers, and photocytotoxic agents.
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spelling pubmed-82013722021-06-15 Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds Hu, Xia Liu, Ning-Yi Deng, Yuan-Qing Wang, Shan Liu, Ting Liu, Xue-Wen Molecules Article The photophysical and biological properties of two new phenanthroline-based ligand ruthenium complexes were investigated in detail. Their DNA interaction modes were determined to be the intercalation mode using spectra titration and viscosity measurements. Under irradiation, obvious photo-reduced DNA cleavages were observed in the two complexes via singlet oxygen generation. Furthermore, complex 2 showed higher DNA affinity, photocleavage activity, and singlet oxygen quantum yields than complex 1. The two complexes showed no toxicity towards tumor cells (HeLa, A549, and A375) in the dark. However, obvious photocytotoxicities were observed in the two complexes. Complex 2 exhibited large PIs (phototherapeutic indices) (ca. 400) towards HeLa cells. The study suggests that these complexes may act as DNA intercalators, DNA photocleavers, and photocytotoxic agents. MDPI 2021-06-07 /pmc/articles/PMC8201372/ /pubmed/34200469 http://dx.doi.org/10.3390/molecules26113471 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hu, Xia
Liu, Ning-Yi
Deng, Yuan-Qing
Wang, Shan
Liu, Ting
Liu, Xue-Wen
Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds
title Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds
title_full Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds
title_fullStr Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds
title_full_unstemmed Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds
title_short Photoinduced DNA Cleavage and Photocytotoxic of Phenanthroline-Based Ligand Ruthenium Compounds
title_sort photoinduced dna cleavage and photocytotoxic of phenanthroline-based ligand ruthenium compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201372/
https://www.ncbi.nlm.nih.gov/pubmed/34200469
http://dx.doi.org/10.3390/molecules26113471
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