Cargando…

Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells

This work shows the ability to remotely control the paracrine performance of mesenchymal stromal cells (MSCs) in producing an angiogenesis key molecule, vascular endothelial growth factor (VEGF-A), by modulation of an external magnetic field. This work compares for the first time the application of...

Descripción completa

Detalles Bibliográficos
Autores principales: Manjua, Ana C., Cabral, Joaquim M. S., Ferreira, Frederico Castelo, Portugal, Carla A. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201388/
https://www.ncbi.nlm.nih.gov/pubmed/34204049
http://dx.doi.org/10.3390/polym13111883
_version_ 1783707807422349312
author Manjua, Ana C.
Cabral, Joaquim M. S.
Ferreira, Frederico Castelo
Portugal, Carla A. M.
author_facet Manjua, Ana C.
Cabral, Joaquim M. S.
Ferreira, Frederico Castelo
Portugal, Carla A. M.
author_sort Manjua, Ana C.
collection PubMed
description This work shows the ability to remotely control the paracrine performance of mesenchymal stromal cells (MSCs) in producing an angiogenesis key molecule, vascular endothelial growth factor (VEGF-A), by modulation of an external magnetic field. This work compares for the first time the application of static and dynamic magnetic fields in angiogenesis in vitro model, exploring the effect of magnetic field intensity and dynamic regimes on the VEGF-A secretion potential of MSCs. Tissue scaffolds of gelatin doped with iron oxide nanoparticles (MNPs) were used as a platform for MSC proliferation. Dynamic magnetic field regimes were imposed by cyclic variation of the magnetic field intensity in different frequencies. The effect of the magnetic field intensity on cell behavior showed that higher intensity of 0.45 T was associated with increased cell death and a poor angiogenic effect. It was observed that static and dynamic magnetic stimulation with higher frequencies led to improved angiogenic performance on endothelial cells in comparison with a lower frequency regime. This work showed the possibility to control VEGF-A secretion by MSCs through modulation of the magnetic field, offering attractive perspectives of a non-invasive therapeutic option for several diseases by revascularizing damaged tissues or inhibiting metastasis formation during cancer progression.
format Online
Article
Text
id pubmed-8201388
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82013882021-06-15 Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells Manjua, Ana C. Cabral, Joaquim M. S. Ferreira, Frederico Castelo Portugal, Carla A. M. Polymers (Basel) Article This work shows the ability to remotely control the paracrine performance of mesenchymal stromal cells (MSCs) in producing an angiogenesis key molecule, vascular endothelial growth factor (VEGF-A), by modulation of an external magnetic field. This work compares for the first time the application of static and dynamic magnetic fields in angiogenesis in vitro model, exploring the effect of magnetic field intensity and dynamic regimes on the VEGF-A secretion potential of MSCs. Tissue scaffolds of gelatin doped with iron oxide nanoparticles (MNPs) were used as a platform for MSC proliferation. Dynamic magnetic field regimes were imposed by cyclic variation of the magnetic field intensity in different frequencies. The effect of the magnetic field intensity on cell behavior showed that higher intensity of 0.45 T was associated with increased cell death and a poor angiogenic effect. It was observed that static and dynamic magnetic stimulation with higher frequencies led to improved angiogenic performance on endothelial cells in comparison with a lower frequency regime. This work showed the possibility to control VEGF-A secretion by MSCs through modulation of the magnetic field, offering attractive perspectives of a non-invasive therapeutic option for several diseases by revascularizing damaged tissues or inhibiting metastasis formation during cancer progression. MDPI 2021-06-06 /pmc/articles/PMC8201388/ /pubmed/34204049 http://dx.doi.org/10.3390/polym13111883 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Manjua, Ana C.
Cabral, Joaquim M. S.
Ferreira, Frederico Castelo
Portugal, Carla A. M.
Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells
title Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells
title_full Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells
title_fullStr Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells
title_full_unstemmed Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells
title_short Magnetic Field Dynamic Strategies for the Improved Control of the Angiogenic Effect of Mesenchymal Stromal Cells
title_sort magnetic field dynamic strategies for the improved control of the angiogenic effect of mesenchymal stromal cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201388/
https://www.ncbi.nlm.nih.gov/pubmed/34204049
http://dx.doi.org/10.3390/polym13111883
work_keys_str_mv AT manjuaanac magneticfielddynamicstrategiesfortheimprovedcontroloftheangiogeniceffectofmesenchymalstromalcells
AT cabraljoaquimms magneticfielddynamicstrategiesfortheimprovedcontroloftheangiogeniceffectofmesenchymalstromalcells
AT ferreirafredericocastelo magneticfielddynamicstrategiesfortheimprovedcontroloftheangiogeniceffectofmesenchymalstromalcells
AT portugalcarlaam magneticfielddynamicstrategiesfortheimprovedcontroloftheangiogeniceffectofmesenchymalstromalcells