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Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli
In recent years, antibiotic-resistant bacteria caused by antibiotic abuse in the medical industry have become a new environmental pollutant that endangers public health. Therefore, it is necessary to establish a detection method for evaluating drug-resistant bacteria. In this work, we used Escherich...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201492/ https://www.ncbi.nlm.nih.gov/pubmed/34136468 http://dx.doi.org/10.3389/fchem.2021.696906 |
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author | Li, Chunlei Sun, Feng |
author_facet | Li, Chunlei Sun, Feng |
author_sort | Li, Chunlei |
collection | PubMed |
description | In recent years, antibiotic-resistant bacteria caused by antibiotic abuse in the medical industry have become a new environmental pollutant that endangers public health. Therefore, it is necessary to establish a detection method for evaluating drug-resistant bacteria. In this work, we used Escherichia coli as a target model and proposed a method to evaluate its drug resistance for three antibiotics. Graphene dispersion was used to co-mix with E. coli cells for the purpose of increasing the current signal. This electrochemical-based sensor allows the evaluation of the activity of E. coli on the electrode surface. When antibiotics were present, the electrocatalytic reduction signal was diminished because of the reduced activity of E. coli. Based on the difference in the electrochemical reduction signal, we can evaluate the antibiotic resistance of different E. coli strains. |
format | Online Article Text |
id | pubmed-8201492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82014922021-06-15 Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli Li, Chunlei Sun, Feng Front Chem Chemistry In recent years, antibiotic-resistant bacteria caused by antibiotic abuse in the medical industry have become a new environmental pollutant that endangers public health. Therefore, it is necessary to establish a detection method for evaluating drug-resistant bacteria. In this work, we used Escherichia coli as a target model and proposed a method to evaluate its drug resistance for three antibiotics. Graphene dispersion was used to co-mix with E. coli cells for the purpose of increasing the current signal. This electrochemical-based sensor allows the evaluation of the activity of E. coli on the electrode surface. When antibiotics were present, the electrocatalytic reduction signal was diminished because of the reduced activity of E. coli. Based on the difference in the electrochemical reduction signal, we can evaluate the antibiotic resistance of different E. coli strains. Frontiers Media S.A. 2021-05-31 /pmc/articles/PMC8201492/ /pubmed/34136468 http://dx.doi.org/10.3389/fchem.2021.696906 Text en Copyright © 2021 Li and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Li, Chunlei Sun, Feng Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli |
title | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli
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title_full | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli
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title_fullStr | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli
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title_full_unstemmed | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli
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title_short | Graphene-Assisted Sensor for Rapid Detection of Antibiotic Resistance in Escherichia coli
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title_sort | graphene-assisted sensor for rapid detection of antibiotic resistance in escherichia coli |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201492/ https://www.ncbi.nlm.nih.gov/pubmed/34136468 http://dx.doi.org/10.3389/fchem.2021.696906 |
work_keys_str_mv | AT lichunlei grapheneassistedsensorforrapiddetectionofantibioticresistanceinescherichiacoli AT sunfeng grapheneassistedsensorforrapiddetectionofantibioticresistanceinescherichiacoli |