Cargando…

KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma

KDF1 has been identified as a key regulator of epidermal proliferation and differentiation, but it is unknown whether KDF1 is involved in the pathogenesis of malignancy. No study has reported the expression and function of KDF1 in renal cancer. To explore the pathologic significance of KDF1 in clear...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Jing-min, Gan, Mei-fu, Yu, Hong-yuan, Ye, Lu-xia, Yu, Qing-xin, Xia, Yu-hui, Zhou, Han-xi, Bao, Jia-qian, Guo, Yi-qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201614/
https://www.ncbi.nlm.nih.gov/pubmed/34136411
http://dx.doi.org/10.3389/fonc.2021.686678
_version_ 1783707840687374336
author Zheng, Jing-min
Gan, Mei-fu
Yu, Hong-yuan
Ye, Lu-xia
Yu, Qing-xin
Xia, Yu-hui
Zhou, Han-xi
Bao, Jia-qian
Guo, Yi-qing
author_facet Zheng, Jing-min
Gan, Mei-fu
Yu, Hong-yuan
Ye, Lu-xia
Yu, Qing-xin
Xia, Yu-hui
Zhou, Han-xi
Bao, Jia-qian
Guo, Yi-qing
author_sort Zheng, Jing-min
collection PubMed
description KDF1 has been identified as a key regulator of epidermal proliferation and differentiation, but it is unknown whether KDF1 is involved in the pathogenesis of malignancy. No study has reported the expression and function of KDF1 in renal cancer. To explore the pathologic significance of KDF1 in clear cell renal cell carcinoma (ccRCC), the expression level of KDF1 protein in the tumor tissue of ccRCC patients was examined by immunohistochemistry and Western blot while the expression level of KDF1 mRNA was analyzed by using the data from TCGA database. In vitro cell experiments and allogeneic tumor transplantation tests were performed to determine the effects of altered KDF1 expression on the phenotype of ccRCC cells. Both the KDF1 mRNA and protein were found to be decreasingly expressed in the tumor tissue of ccRCC patients when compared with the adjacent non-tumor control tissue. The expression level of KDF1 in the tumor tissue was found to correlate negatively with the tumor grade. Patients with higher KDF1 in the tumor tissue were found to have longer overall survival and disease-specific survival time. KDF1 was shown to be an independent factor influencing the disease-specific survival of the ccRCC patients. Overexpression of KDF1 was found to inhibit the proliferation, migration and invasion of ccRCC cells, which could be reversed by decreasing the expression of KDF1 again. ccRCC cells with KDF1 overexpression were found to produce smaller transgrafted tumors. These results support the idea that KDF1 is involved in ccRCC and may function as a tumor suppressor.
format Online
Article
Text
id pubmed-8201614
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-82016142021-06-15 KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma Zheng, Jing-min Gan, Mei-fu Yu, Hong-yuan Ye, Lu-xia Yu, Qing-xin Xia, Yu-hui Zhou, Han-xi Bao, Jia-qian Guo, Yi-qing Front Oncol Oncology KDF1 has been identified as a key regulator of epidermal proliferation and differentiation, but it is unknown whether KDF1 is involved in the pathogenesis of malignancy. No study has reported the expression and function of KDF1 in renal cancer. To explore the pathologic significance of KDF1 in clear cell renal cell carcinoma (ccRCC), the expression level of KDF1 protein in the tumor tissue of ccRCC patients was examined by immunohistochemistry and Western blot while the expression level of KDF1 mRNA was analyzed by using the data from TCGA database. In vitro cell experiments and allogeneic tumor transplantation tests were performed to determine the effects of altered KDF1 expression on the phenotype of ccRCC cells. Both the KDF1 mRNA and protein were found to be decreasingly expressed in the tumor tissue of ccRCC patients when compared with the adjacent non-tumor control tissue. The expression level of KDF1 in the tumor tissue was found to correlate negatively with the tumor grade. Patients with higher KDF1 in the tumor tissue were found to have longer overall survival and disease-specific survival time. KDF1 was shown to be an independent factor influencing the disease-specific survival of the ccRCC patients. Overexpression of KDF1 was found to inhibit the proliferation, migration and invasion of ccRCC cells, which could be reversed by decreasing the expression of KDF1 again. ccRCC cells with KDF1 overexpression were found to produce smaller transgrafted tumors. These results support the idea that KDF1 is involved in ccRCC and may function as a tumor suppressor. Frontiers Media S.A. 2021-05-31 /pmc/articles/PMC8201614/ /pubmed/34136411 http://dx.doi.org/10.3389/fonc.2021.686678 Text en Copyright © 2021 Zheng, Gan, Yu, Ye, Yu, Xia, Zhou, Bao and Guo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Zheng, Jing-min
Gan, Mei-fu
Yu, Hong-yuan
Ye, Lu-xia
Yu, Qing-xin
Xia, Yu-hui
Zhou, Han-xi
Bao, Jia-qian
Guo, Yi-qing
KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma
title KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma
title_full KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma
title_fullStr KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma
title_full_unstemmed KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma
title_short KDF1, a Novel Tumor Suppressor in Clear Cell Renal Cell Carcinoma
title_sort kdf1, a novel tumor suppressor in clear cell renal cell carcinoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201614/
https://www.ncbi.nlm.nih.gov/pubmed/34136411
http://dx.doi.org/10.3389/fonc.2021.686678
work_keys_str_mv AT zhengjingmin kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT ganmeifu kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT yuhongyuan kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT yeluxia kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT yuqingxin kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT xiayuhui kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT zhouhanxi kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT baojiaqian kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma
AT guoyiqing kdf1anoveltumorsuppressorinclearcellrenalcellcarcinoma