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A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli

Due to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of dru...

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Detalles Bibliográficos
Autores principales: Duan, Rongshuai, Fang, Xiao, Wang, Dongliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201616/
https://www.ncbi.nlm.nih.gov/pubmed/34136465
http://dx.doi.org/10.3389/fchem.2021.689735
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author Duan, Rongshuai
Fang, Xiao
Wang, Dongliang
author_facet Duan, Rongshuai
Fang, Xiao
Wang, Dongliang
author_sort Duan, Rongshuai
collection PubMed
description Due to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of drug-resistant bacteria. Using methylene blue as a redox probe, the electrochemical signals of methylene blue in drug-resistant Escherichia coli strains were analyzed by a CV method. Graphene ink has been used for enhancing the electrochemical signal. Compared with the results of the traditional drug sensitivity test, we proposed a rapid electrochemical drug sensitivity test method which can effectively identify the drug sensitivity of Escherichia coli. The sensitivity of four E. coli isolates to ciprofloxacin, gentamicin, and ampicillin was tested by an electrochemical drug sensitivity test. The respiratory activity value %RA was used as an indicator of bacterial resistance by electrochemical method.
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spelling pubmed-82016162021-06-15 A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli Duan, Rongshuai Fang, Xiao Wang, Dongliang Front Chem Chemistry Due to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of drug-resistant bacteria. Using methylene blue as a redox probe, the electrochemical signals of methylene blue in drug-resistant Escherichia coli strains were analyzed by a CV method. Graphene ink has been used for enhancing the electrochemical signal. Compared with the results of the traditional drug sensitivity test, we proposed a rapid electrochemical drug sensitivity test method which can effectively identify the drug sensitivity of Escherichia coli. The sensitivity of four E. coli isolates to ciprofloxacin, gentamicin, and ampicillin was tested by an electrochemical drug sensitivity test. The respiratory activity value %RA was used as an indicator of bacterial resistance by electrochemical method. Frontiers Media S.A. 2021-05-31 /pmc/articles/PMC8201616/ /pubmed/34136465 http://dx.doi.org/10.3389/fchem.2021.689735 Text en Copyright © 2021 Duan, Fang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Duan, Rongshuai
Fang, Xiao
Wang, Dongliang
A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli
title A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli
title_full A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli
title_fullStr A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli
title_full_unstemmed A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli
title_short A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli
title_sort methylene blue assisted electrochemical sensor for determination of drug resistance of escherichia coli
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201616/
https://www.ncbi.nlm.nih.gov/pubmed/34136465
http://dx.doi.org/10.3389/fchem.2021.689735
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