Accessible and Validated Processing of SARS-CoV-2 from Wastewater

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19), is shed into wastewater. Accessible methods are necessary for processing samples in a myriad of contexts. We optimized a protocol for extracting viral RNA for downstream experi...

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Detalles Bibliográficos
Autores principales: Rondeau, Nicole C., Rose, Oliver J., Ariyan, Lina A., Mailloux, Brian J., Miranda, JJ L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Protocols and Workflows
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201630/
https://www.ncbi.nlm.nih.gov/pubmed/34042474
http://dx.doi.org/10.1128/MRA.00174-21
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author Rondeau, Nicole C.
Rose, Oliver J.
Ariyan, Lina A.
Mailloux, Brian J.
Miranda, JJ L.
author_facet Rondeau, Nicole C.
Rose, Oliver J.
Ariyan, Lina A.
Mailloux, Brian J.
Miranda, JJ L.
author_sort Rondeau, Nicole C.
collection PubMed
description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19), is shed into wastewater. Accessible methods are necessary for processing samples in a myriad of contexts. We optimized a protocol for extracting viral RNA for downstream experiments. Our pipeline was validated with SARS-CoV-2 itself as a matrix recovery and quantitative measurement control.
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spelling pubmed-82016302021-07-02 Accessible and Validated Processing of SARS-CoV-2 from Wastewater Rondeau, Nicole C. Rose, Oliver J. Ariyan, Lina A. Mailloux, Brian J. Miranda, JJ L. Microbiol Resour Announc Protocols and Workflows Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19), is shed into wastewater. Accessible methods are necessary for processing samples in a myriad of contexts. We optimized a protocol for extracting viral RNA for downstream experiments. Our pipeline was validated with SARS-CoV-2 itself as a matrix recovery and quantitative measurement control. American Society for Microbiology 2021-05-27 /pmc/articles/PMC8201630/ /pubmed/34042474 http://dx.doi.org/10.1128/MRA.00174-21 Text en Copyright © 2021 Rondeau et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Protocols and Workflows
Rondeau, Nicole C.
Rose, Oliver J.
Ariyan, Lina A.
Mailloux, Brian J.
Miranda, JJ L.
Accessible and Validated Processing of SARS-CoV-2 from Wastewater
title Accessible and Validated Processing of SARS-CoV-2 from Wastewater
title_full Accessible and Validated Processing of SARS-CoV-2 from Wastewater
title_fullStr Accessible and Validated Processing of SARS-CoV-2 from Wastewater
title_full_unstemmed Accessible and Validated Processing of SARS-CoV-2 from Wastewater
title_short Accessible and Validated Processing of SARS-CoV-2 from Wastewater
title_sort accessible and validated processing of sars-cov-2 from wastewater
topic Protocols and Workflows
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201630/
https://www.ncbi.nlm.nih.gov/pubmed/34042474
http://dx.doi.org/10.1128/MRA.00174-21
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