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Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease
BACKGROUND: Kawasaki disease (KD) is the main cause of acquired heart disease in children and can lead to coronary artery lesions. This present study was designed to analyze the characteristics of KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequencing (scRNA-seq) and to expl...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201934/ https://www.ncbi.nlm.nih.gov/pubmed/34126969 http://dx.doi.org/10.1186/s12887-021-02754-5 |
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author | Fan, Xue Zhou, Yuhan Guo, Xin Xu, Mingguo |
author_facet | Fan, Xue Zhou, Yuhan Guo, Xin Xu, Mingguo |
author_sort | Fan, Xue |
collection | PubMed |
description | BACKGROUND: Kawasaki disease (KD) is the main cause of acquired heart disease in children and can lead to coronary artery lesions. This present study was designed to analyze the characteristics of KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequencing (scRNA-seq) and to explore the potential molecular mechanism of KD. METHODS: PBMC was collected from one healthy child and one KD patient, and was used to single-cell RNA sequencing for cell clusters identification and differently expressed gene (DEG) determination. GO function enrichment analysis of DEG in B cell and T cells were performed to explore the most active biological function in KD immune cells. RESULTS: Twelve cell clusters can be identified in two samples. Compared with healthy child, naive CD8+ T cell, T helper cell and B cell in KD child were decreased, mainly immune-related T cells, and natural killer T (NKT) cell were increased. Cell activation, lymphocyte activation and regulation of immune system process were 3 GO function shared by all four types of T cells and B cell. CONCLUSIONS: Immune cell disorder appears in the KD patient at single cell level by scRNA-seq. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12887-021-02754-5. |
format | Online Article Text |
id | pubmed-8201934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-82019342021-06-16 Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease Fan, Xue Zhou, Yuhan Guo, Xin Xu, Mingguo BMC Pediatr Research BACKGROUND: Kawasaki disease (KD) is the main cause of acquired heart disease in children and can lead to coronary artery lesions. This present study was designed to analyze the characteristics of KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequencing (scRNA-seq) and to explore the potential molecular mechanism of KD. METHODS: PBMC was collected from one healthy child and one KD patient, and was used to single-cell RNA sequencing for cell clusters identification and differently expressed gene (DEG) determination. GO function enrichment analysis of DEG in B cell and T cells were performed to explore the most active biological function in KD immune cells. RESULTS: Twelve cell clusters can be identified in two samples. Compared with healthy child, naive CD8+ T cell, T helper cell and B cell in KD child were decreased, mainly immune-related T cells, and natural killer T (NKT) cell were increased. Cell activation, lymphocyte activation and regulation of immune system process were 3 GO function shared by all four types of T cells and B cell. CONCLUSIONS: Immune cell disorder appears in the KD patient at single cell level by scRNA-seq. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12887-021-02754-5. BioMed Central 2021-06-14 /pmc/articles/PMC8201934/ /pubmed/34126969 http://dx.doi.org/10.1186/s12887-021-02754-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Fan, Xue Zhou, Yuhan Guo, Xin Xu, Mingguo Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease |
title | Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease |
title_full | Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease |
title_fullStr | Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease |
title_full_unstemmed | Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease |
title_short | Utilizing single-cell RNA sequencing for analyzing the characteristics of PBMC in patients with Kawasaki disease |
title_sort | utilizing single-cell rna sequencing for analyzing the characteristics of pbmc in patients with kawasaki disease |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201934/ https://www.ncbi.nlm.nih.gov/pubmed/34126969 http://dx.doi.org/10.1186/s12887-021-02754-5 |
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