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Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China

Migratory birds are recently recognized as Vibrio disease vectors, but may be widespread transporters of Vibrio strains. We isolated Vibrio cholerae (V. cholerae) and Vibrio metschnikovii (V. metschnikovii) strains from migratory bird epidemic samples from 2017 to 2018 and isolated V. metschnikovii...

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Autores principales: Zheng, Lin, Zhu, Ling-Wei, Jing, Jie, Guan, Jia-yao, Lu, Ge-Jin, Xie, Lin-Hong, Ji, Xue, Chu, Dong, Sun, Yang, Chen, Ping, Guo, Xue-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202012/
https://www.ncbi.nlm.nih.gov/pubmed/34136552
http://dx.doi.org/10.3389/fvets.2021.638820
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author Zheng, Lin
Zhu, Ling-Wei
Jing, Jie
Guan, Jia-yao
Lu, Ge-Jin
Xie, Lin-Hong
Ji, Xue
Chu, Dong
Sun, Yang
Chen, Ping
Guo, Xue-Jun
author_facet Zheng, Lin
Zhu, Ling-Wei
Jing, Jie
Guan, Jia-yao
Lu, Ge-Jin
Xie, Lin-Hong
Ji, Xue
Chu, Dong
Sun, Yang
Chen, Ping
Guo, Xue-Jun
author_sort Zheng, Lin
collection PubMed
description Migratory birds are recently recognized as Vibrio disease vectors, but may be widespread transporters of Vibrio strains. We isolated Vibrio cholerae (V. cholerae) and Vibrio metschnikovii (V. metschnikovii) strains from migratory bird epidemic samples from 2017 to 2018 and isolated V. metschnikovii from migratory bird feces in 2019 from bird samples taken from the Inner Mongolia autonomous region of China. To investigate the evolution of these two Vibrio species, we sequenced the genomes of 40 V. cholerae strains and 34 V. metschnikovii strains isolated from the bird samples and compared these genomes with reference strain genomes. The pan-genome of all V. cholerae and V. metschnikovii genomes was large, with strains exhibiting considerable individual differences. A total of 2,130 and 1,352 core genes were identified in the V. cholerae and V. metschnikovii genomes, respectively, while dispensable genes accounted for 16,180 and 9,178 of all genes for the two strains, respectively. All V. cholerae strains isolated from the migratory birds that encoded T6SS and hlyA were non-O1/O139 serotypes without the ability to produce CTX. These strains also lacked the ability to produce the TCP fimbriae nor the extracellular matrix protein RbmA and could not metabolize trimetlylamine oxide (TMAO). Thus, these characteristics render them unlikely to be pandemic-inducing strains. However, a V. metschnikovii isolate encoding the complete T6SS system was isolated for the first time. These data provide new molecular insights into the diversity of V. cholerae and V. metschnikovii isolates recovered from migratory birds.
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spelling pubmed-82020122021-06-15 Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China Zheng, Lin Zhu, Ling-Wei Jing, Jie Guan, Jia-yao Lu, Ge-Jin Xie, Lin-Hong Ji, Xue Chu, Dong Sun, Yang Chen, Ping Guo, Xue-Jun Front Vet Sci Veterinary Science Migratory birds are recently recognized as Vibrio disease vectors, but may be widespread transporters of Vibrio strains. We isolated Vibrio cholerae (V. cholerae) and Vibrio metschnikovii (V. metschnikovii) strains from migratory bird epidemic samples from 2017 to 2018 and isolated V. metschnikovii from migratory bird feces in 2019 from bird samples taken from the Inner Mongolia autonomous region of China. To investigate the evolution of these two Vibrio species, we sequenced the genomes of 40 V. cholerae strains and 34 V. metschnikovii strains isolated from the bird samples and compared these genomes with reference strain genomes. The pan-genome of all V. cholerae and V. metschnikovii genomes was large, with strains exhibiting considerable individual differences. A total of 2,130 and 1,352 core genes were identified in the V. cholerae and V. metschnikovii genomes, respectively, while dispensable genes accounted for 16,180 and 9,178 of all genes for the two strains, respectively. All V. cholerae strains isolated from the migratory birds that encoded T6SS and hlyA were non-O1/O139 serotypes without the ability to produce CTX. These strains also lacked the ability to produce the TCP fimbriae nor the extracellular matrix protein RbmA and could not metabolize trimetlylamine oxide (TMAO). Thus, these characteristics render them unlikely to be pandemic-inducing strains. However, a V. metschnikovii isolate encoding the complete T6SS system was isolated for the first time. These data provide new molecular insights into the diversity of V. cholerae and V. metschnikovii isolates recovered from migratory birds. Frontiers Media S.A. 2021-05-31 /pmc/articles/PMC8202012/ /pubmed/34136552 http://dx.doi.org/10.3389/fvets.2021.638820 Text en Copyright © 2021 Zheng, Zhu, Jing, Guan, Lu, Xie, Ji, Chu, Sun, Chen and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Zheng, Lin
Zhu, Ling-Wei
Jing, Jie
Guan, Jia-yao
Lu, Ge-Jin
Xie, Lin-Hong
Ji, Xue
Chu, Dong
Sun, Yang
Chen, Ping
Guo, Xue-Jun
Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China
title Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China
title_full Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China
title_fullStr Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China
title_full_unstemmed Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China
title_short Pan-Genome Analysis of Vibrio cholerae and Vibrio metschnikovii Strains Isolated From Migratory Birds at Dali Nouer Lake in Chifeng, China
title_sort pan-genome analysis of vibrio cholerae and vibrio metschnikovii strains isolated from migratory birds at dali nouer lake in chifeng, china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202012/
https://www.ncbi.nlm.nih.gov/pubmed/34136552
http://dx.doi.org/10.3389/fvets.2021.638820
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