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Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo
Relatively little is known about features of T cells targeted by HIV in vivo. By applying bioinformatics analysis to mass cytometry (CyTOF)-phenotyped specimens from individuals with viremia and in-vitro-infected cells from uninfected donors, we provide an atlas of the phenotypes of in vivo and in v...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202093/ https://www.ncbi.nlm.nih.gov/pubmed/33910003 http://dx.doi.org/10.1016/j.celrep.2021.109038 |
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author | Xie, Guorui Luo, Xiaoyu Ma, Tongcui Frouard, Julie Neidleman, Jason Hoh, Rebecca Deeks, Steven G. Greene, Warner C. Roan, Nadia R. |
author_facet | Xie, Guorui Luo, Xiaoyu Ma, Tongcui Frouard, Julie Neidleman, Jason Hoh, Rebecca Deeks, Steven G. Greene, Warner C. Roan, Nadia R. |
author_sort | Xie, Guorui |
collection | PubMed |
description | Relatively little is known about features of T cells targeted by HIV in vivo. By applying bioinformatics analysis to mass cytometry (CyTOF)-phenotyped specimens from individuals with viremia and in-vitro-infected cells from uninfected donors, we provide an atlas of the phenotypes of in vivo and in vitro HIV-susceptible cells. T helper 17 (Th17) and α4β1(+) cells are preferentially targeted in vivo, whereas T effector memory (Tem), T transitional memory (Ttm), Th1, and Th1/Th17 subsets are targeted in vitro. Multiple proteins—including chemokine and cytokine receptors—are remodeled by HIV in vivo, and these changes are mostly recapitulated in vitro. HIV remodels cells to a T follicular helper (Tfh) phenotype. Using clustering, we uncover a subset of CD29-expressing, Tem-like cells that are highly susceptible to infection in vivo and in vitro and experimentally confirm that susceptibility. These studies provide an in-depth look at features of HIV-susceptible cells in individuals with viremia and demonstrate that some—but not all—HIV-susceptible cells identified in vitro effectively model in vivo susceptibility. |
format | Online Article Text |
id | pubmed-8202093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-82020932021-06-14 Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo Xie, Guorui Luo, Xiaoyu Ma, Tongcui Frouard, Julie Neidleman, Jason Hoh, Rebecca Deeks, Steven G. Greene, Warner C. Roan, Nadia R. Cell Rep Article Relatively little is known about features of T cells targeted by HIV in vivo. By applying bioinformatics analysis to mass cytometry (CyTOF)-phenotyped specimens from individuals with viremia and in-vitro-infected cells from uninfected donors, we provide an atlas of the phenotypes of in vivo and in vitro HIV-susceptible cells. T helper 17 (Th17) and α4β1(+) cells are preferentially targeted in vivo, whereas T effector memory (Tem), T transitional memory (Ttm), Th1, and Th1/Th17 subsets are targeted in vitro. Multiple proteins—including chemokine and cytokine receptors—are remodeled by HIV in vivo, and these changes are mostly recapitulated in vitro. HIV remodels cells to a T follicular helper (Tfh) phenotype. Using clustering, we uncover a subset of CD29-expressing, Tem-like cells that are highly susceptible to infection in vivo and in vitro and experimentally confirm that susceptibility. These studies provide an in-depth look at features of HIV-susceptible cells in individuals with viremia and demonstrate that some—but not all—HIV-susceptible cells identified in vitro effectively model in vivo susceptibility. 2021-04-27 /pmc/articles/PMC8202093/ /pubmed/33910003 http://dx.doi.org/10.1016/j.celrep.2021.109038 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Xie, Guorui Luo, Xiaoyu Ma, Tongcui Frouard, Julie Neidleman, Jason Hoh, Rebecca Deeks, Steven G. Greene, Warner C. Roan, Nadia R. Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo |
title | Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo |
title_full | Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo |
title_fullStr | Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo |
title_full_unstemmed | Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo |
title_short | Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4(+) T cell subsets in vivo |
title_sort | characterization of hiv-induced remodeling reveals differences in infection susceptibility of memory cd4(+) t cell subsets in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202093/ https://www.ncbi.nlm.nih.gov/pubmed/33910003 http://dx.doi.org/10.1016/j.celrep.2021.109038 |
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