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Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion

BACKGROUND: Renal ischemia-reperfusion (I/R) is directly associated with acute renal failure and can occur in conditions such as infarction caused by embolization or thrombosis, septicemia, and kidney transplantation. The process is complex, involving innate and adaptive immune responses, presence o...

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Autores principales: Rossetti, Leandro Pablos, da Costa, Larissa Bastos Eloy, Guillaumon, Ana Terezinha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Angiologia e de Cirurgia Vascular (SBACV) 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202179/
https://www.ncbi.nlm.nih.gov/pubmed/34178047
http://dx.doi.org/10.1590/1677-5449.190010
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author Rossetti, Leandro Pablos
da Costa, Larissa Bastos Eloy
Guillaumon, Ana Terezinha
author_facet Rossetti, Leandro Pablos
da Costa, Larissa Bastos Eloy
Guillaumon, Ana Terezinha
author_sort Rossetti, Leandro Pablos
collection PubMed
description BACKGROUND: Renal ischemia-reperfusion (I/R) is directly associated with acute renal failure and can occur in conditions such as infarction caused by embolization or thrombosis, septicemia, and kidney transplantation. The process is complex, involving innate and adaptive immune responses, presence of cellular infiltrate, and production and release of cytokines and chemokines. It also triggers cell responses and release of reactive oxygen species, in addition to causing apoptosis and, in some cases, cell necrosis. Against this background, evaluation of renal tissue protection mechanisms is essential. OBJECTIVES: The objective of this study was to test the M&G solution, developed in prior research, evaluating its capacity to protect the kidneys using morphometric analysis and by assaying the presence and expression of inflammatory cytokines (TNF-alpha, VEGF, HIF, and IL-8). METHODS: Eighteen Wistar rats were divided into three groups: Sham (S), Control (C), and Experimental (E). The S group underwent the surgical operation, but without arterial clamping. In group C, the aorta was clamped above and below the left renal artery, without infusion of the preservation solution. In group E, in addition to clamping, the aorta was punctured and M&G solution was infused continuously for 20 minutes at 15(o) C. Morphological analysis and immunohistochemical assessment of markers were then conducted. RESULTS: Morphological differences were identified in group S compared with groups C and E. Analysis of markers revealed reduced intensity of expression of TNF and of VEGF in group E. There were no differences in HIF or IL-8 between groups. CONCLUSIONS: The M&G solution was associated with a reduction in presence and expression of TNF-alpha and a trend to reduced VEGF.
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spelling pubmed-82021792021-06-24 Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion Rossetti, Leandro Pablos da Costa, Larissa Bastos Eloy Guillaumon, Ana Terezinha J Vasc Bras Original Article BACKGROUND: Renal ischemia-reperfusion (I/R) is directly associated with acute renal failure and can occur in conditions such as infarction caused by embolization or thrombosis, septicemia, and kidney transplantation. The process is complex, involving innate and adaptive immune responses, presence of cellular infiltrate, and production and release of cytokines and chemokines. It also triggers cell responses and release of reactive oxygen species, in addition to causing apoptosis and, in some cases, cell necrosis. Against this background, evaluation of renal tissue protection mechanisms is essential. OBJECTIVES: The objective of this study was to test the M&G solution, developed in prior research, evaluating its capacity to protect the kidneys using morphometric analysis and by assaying the presence and expression of inflammatory cytokines (TNF-alpha, VEGF, HIF, and IL-8). METHODS: Eighteen Wistar rats were divided into three groups: Sham (S), Control (C), and Experimental (E). The S group underwent the surgical operation, but without arterial clamping. In group C, the aorta was clamped above and below the left renal artery, without infusion of the preservation solution. In group E, in addition to clamping, the aorta was punctured and M&G solution was infused continuously for 20 minutes at 15(o) C. Morphological analysis and immunohistochemical assessment of markers were then conducted. RESULTS: Morphological differences were identified in group S compared with groups C and E. Analysis of markers revealed reduced intensity of expression of TNF and of VEGF in group E. There were no differences in HIF or IL-8 between groups. CONCLUSIONS: The M&G solution was associated with a reduction in presence and expression of TNF-alpha and a trend to reduced VEGF. Sociedade Brasileira de Angiologia e de Cirurgia Vascular (SBACV) 2020-06-06 /pmc/articles/PMC8202179/ /pubmed/34178047 http://dx.doi.org/10.1590/1677-5449.190010 Text en https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rossetti, Leandro Pablos
da Costa, Larissa Bastos Eloy
Guillaumon, Ana Terezinha
Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion
title Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion
title_full Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion
title_fullStr Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion
title_full_unstemmed Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion
title_short Effect of infusion of M&G solution for protection of renal tissue in Wistar rats subjected to programmed ischemia-reperfusion
title_sort effect of infusion of m&g solution for protection of renal tissue in wistar rats subjected to programmed ischemia-reperfusion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202179/
https://www.ncbi.nlm.nih.gov/pubmed/34178047
http://dx.doi.org/10.1590/1677-5449.190010
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