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Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA du...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202426/ https://www.ncbi.nlm.nih.gov/pubmed/34127974 http://dx.doi.org/10.1101/2021.06.09.447760 |
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author | Wang, Jiarui Han, Mengting Roy, Anish R. Wang, Haifeng Möckl, Leonhard Zeng, Leiping Moerner, W. E. Qi, Lei S. |
author_facet | Wang, Jiarui Han, Mengting Roy, Anish R. Wang, Haifeng Möckl, Leonhard Zeng, Leiping Moerner, W. E. Qi, Lei S. |
author_sort | Wang, Jiarui |
collection | PubMed |
description | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects. |
format | Online Article Text |
id | pubmed-8202426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-82024262021-06-15 Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection Wang, Jiarui Han, Mengting Roy, Anish R. Wang, Haifeng Möckl, Leonhard Zeng, Leiping Moerner, W. E. Qi, Lei S. bioRxiv Article The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects. Cold Spring Harbor Laboratory 2022-01-12 /pmc/articles/PMC8202426/ /pubmed/34127974 http://dx.doi.org/10.1101/2021.06.09.447760 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Wang, Jiarui Han, Mengting Roy, Anish R. Wang, Haifeng Möckl, Leonhard Zeng, Leiping Moerner, W. E. Qi, Lei S. Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |
title | Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |
title_full | Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |
title_fullStr | Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |
title_full_unstemmed | Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |
title_short | Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |
title_sort | multi-color super-resolution imaging to study human coronavirus rna during cellular infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202426/ https://www.ncbi.nlm.nih.gov/pubmed/34127974 http://dx.doi.org/10.1101/2021.06.09.447760 |
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