Cargando…

Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA du...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jiarui, Han, Mengting, Roy, Anish R., Wang, Haifeng, Möckl, Leonhard, Zeng, Leiping, Moerner, W. E., Qi, Lei S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202426/
https://www.ncbi.nlm.nih.gov/pubmed/34127974
http://dx.doi.org/10.1101/2021.06.09.447760
_version_ 1783707980448923648
author Wang, Jiarui
Han, Mengting
Roy, Anish R.
Wang, Haifeng
Möckl, Leonhard
Zeng, Leiping
Moerner, W. E.
Qi, Lei S.
author_facet Wang, Jiarui
Han, Mengting
Roy, Anish R.
Wang, Haifeng
Möckl, Leonhard
Zeng, Leiping
Moerner, W. E.
Qi, Lei S.
author_sort Wang, Jiarui
collection PubMed
description The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects.
format Online
Article
Text
id pubmed-8202426
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-82024262021-06-15 Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection Wang, Jiarui Han, Mengting Roy, Anish R. Wang, Haifeng Möckl, Leonhard Zeng, Leiping Moerner, W. E. Qi, Lei S. bioRxiv Article The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects. Cold Spring Harbor Laboratory 2022-01-12 /pmc/articles/PMC8202426/ /pubmed/34127974 http://dx.doi.org/10.1101/2021.06.09.447760 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Wang, Jiarui
Han, Mengting
Roy, Anish R.
Wang, Haifeng
Möckl, Leonhard
Zeng, Leiping
Moerner, W. E.
Qi, Lei S.
Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
title Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
title_full Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
title_fullStr Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
title_full_unstemmed Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
title_short Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection
title_sort multi-color super-resolution imaging to study human coronavirus rna during cellular infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202426/
https://www.ncbi.nlm.nih.gov/pubmed/34127974
http://dx.doi.org/10.1101/2021.06.09.447760
work_keys_str_mv AT wangjiarui multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection
AT hanmengting multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection
AT royanishr multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection
AT wanghaifeng multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection
AT mocklleonhard multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection
AT zengleiping multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection
AT moernerwe multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection
AT qileis multicolorsuperresolutionimagingtostudyhumancoronavirusrnaduringcellularinfection