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Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p

Long intergenic noncoding RNAs (lincRNAs) regulate a series of biological processes, and their anomalous expression plays critical roles in the progression of multiple malignancies, including colorectal cancer (CRC). Although many studies have reported the oncogenic function of LINC00665 in multiple...

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Autores principales: Wu, Chang-Liang, Shan, Ti-Dong, Han, Yue, Kong, Yan, Li, Yuan-Bo, Peng, Xin-Gang, Shang, Liang, Wang, Pei-Ge, Li, Le-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202867/
https://www.ncbi.nlm.nih.gov/pubmed/33878735
http://dx.doi.org/10.18632/aging.202874
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author Wu, Chang-Liang
Shan, Ti-Dong
Han, Yue
Kong, Yan
Li, Yuan-Bo
Peng, Xin-Gang
Shang, Liang
Wang, Pei-Ge
Li, Le-Ping
author_facet Wu, Chang-Liang
Shan, Ti-Dong
Han, Yue
Kong, Yan
Li, Yuan-Bo
Peng, Xin-Gang
Shang, Liang
Wang, Pei-Ge
Li, Le-Ping
author_sort Wu, Chang-Liang
collection PubMed
description Long intergenic noncoding RNAs (lincRNAs) regulate a series of biological processes, and their anomalous expression plays critical roles in the progression of multiple malignancies, including colorectal cancer (CRC). Although many studies have reported the oncogenic function of LINC00665 in multiple cancers, few studies have explored its role in CRC. The aim of this study was to assess the effect of LINC00665 on the malignant behaviors of CRC and explore the underlying regulatory mechanism of LINC00665. LINC00665 was significantly upregulated in CRC. A loss-of-function assay revealed that LINC00665 downregulation inhibited the proliferation and promoted the apoptosis of CRC cells, which was mediated by cyclin D1, CDK4, caspase-9 and caspase-3. Through mechanistic exploration, we found that miR-126-5p directly bound to LINC00665. Moreover, LINC00665 and miR-126-5p both regulated PAK2 and FZD3 expression. Mechanistically, miR-126-5p was predicted and further verified as a target of both PAK2 and FZD3. These findings demonstrate that LINC00665 might play an important pro-proliferative and antiapoptotic role in CRC and might be a potential biomarker and a new therapeutic target for CRC.
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spelling pubmed-82028672021-06-15 Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p Wu, Chang-Liang Shan, Ti-Dong Han, Yue Kong, Yan Li, Yuan-Bo Peng, Xin-Gang Shang, Liang Wang, Pei-Ge Li, Le-Ping Aging (Albany NY) Research Paper Long intergenic noncoding RNAs (lincRNAs) regulate a series of biological processes, and their anomalous expression plays critical roles in the progression of multiple malignancies, including colorectal cancer (CRC). Although many studies have reported the oncogenic function of LINC00665 in multiple cancers, few studies have explored its role in CRC. The aim of this study was to assess the effect of LINC00665 on the malignant behaviors of CRC and explore the underlying regulatory mechanism of LINC00665. LINC00665 was significantly upregulated in CRC. A loss-of-function assay revealed that LINC00665 downregulation inhibited the proliferation and promoted the apoptosis of CRC cells, which was mediated by cyclin D1, CDK4, caspase-9 and caspase-3. Through mechanistic exploration, we found that miR-126-5p directly bound to LINC00665. Moreover, LINC00665 and miR-126-5p both regulated PAK2 and FZD3 expression. Mechanistically, miR-126-5p was predicted and further verified as a target of both PAK2 and FZD3. These findings demonstrate that LINC00665 might play an important pro-proliferative and antiapoptotic role in CRC and might be a potential biomarker and a new therapeutic target for CRC. Impact Journals 2021-04-20 /pmc/articles/PMC8202867/ /pubmed/33878735 http://dx.doi.org/10.18632/aging.202874 Text en Copyright: © 2021 Wu et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wu, Chang-Liang
Shan, Ti-Dong
Han, Yue
Kong, Yan
Li, Yuan-Bo
Peng, Xin-Gang
Shang, Liang
Wang, Pei-Ge
Li, Le-Ping
Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p
title Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p
title_full Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p
title_fullStr Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p
title_full_unstemmed Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p
title_short Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p
title_sort long intergenic noncoding rna 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating mir-126-5p
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202867/
https://www.ncbi.nlm.nih.gov/pubmed/33878735
http://dx.doi.org/10.18632/aging.202874
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