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IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation

Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to brea...

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Autores principales: Lee, Seounghun, Shin, Hyo-Jung, Noh, Chan, Kim, Song-I, Ko, Young-Kwon, Lee, Sun-Yeul, Lim, Chaeseong, Hong, Boohwi, Yang, Sin-Young, Kim, Dong-Woon, Lee, Won-Hyung, Kim, Yoon-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203094/
https://www.ncbi.nlm.nih.gov/pubmed/34073390
http://dx.doi.org/10.3390/ijms22115657
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author Lee, Seounghun
Shin, Hyo-Jung
Noh, Chan
Kim, Song-I
Ko, Young-Kwon
Lee, Sun-Yeul
Lim, Chaeseong
Hong, Boohwi
Yang, Sin-Young
Kim, Dong-Woon
Lee, Won-Hyung
Kim, Yoon-Hee
author_facet Lee, Seounghun
Shin, Hyo-Jung
Noh, Chan
Kim, Song-I
Ko, Young-Kwon
Lee, Sun-Yeul
Lim, Chaeseong
Hong, Boohwi
Yang, Sin-Young
Kim, Dong-Woon
Lee, Won-Hyung
Kim, Yoon-Hee
author_sort Lee, Seounghun
collection PubMed
description Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to break down IκB, which blocks the activity of NF-kB. Therefore, it prevents the activity of NK-kB. This study investigated whether neuropathic pain can be reduced in spinal nerve ligation (SNL) rats by reducing the activity of microglia by delivering IKBKB small interfering RNA (siRNA)-encapsulated poly (lactic-co-glycolic acid) (PLGA) nanoparticles. PLGA nanoparticles, as a carrier for the delivery of IKBKB genes silencer, were used because they have shown potential to enhance microglial targeting. SNL rats were injected with IKBKB siRNA-encapsulated PLGA nanoparticles intrathecally for behavioral tests on pain response. IKBKB siRNA was delivered for suppressing the expression of IKBKB. In rats injected with IKBKB siRNA-encapsulated PLGA nanoparticles, allodynia caused by mechanical stimulation was reduced, and the secretion of pro-inflammatory mediators due to NF-κB was reduced. Delivering IKBKB siRNA through PLGA nanoparticles can effectively control the inflammatory response and is worth studying as a treatment for neuropathic pain.
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spelling pubmed-82030942021-06-15 IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation Lee, Seounghun Shin, Hyo-Jung Noh, Chan Kim, Song-I Ko, Young-Kwon Lee, Sun-Yeul Lim, Chaeseong Hong, Boohwi Yang, Sin-Young Kim, Dong-Woon Lee, Won-Hyung Kim, Yoon-Hee Int J Mol Sci Article Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to break down IκB, which blocks the activity of NF-kB. Therefore, it prevents the activity of NK-kB. This study investigated whether neuropathic pain can be reduced in spinal nerve ligation (SNL) rats by reducing the activity of microglia by delivering IKBKB small interfering RNA (siRNA)-encapsulated poly (lactic-co-glycolic acid) (PLGA) nanoparticles. PLGA nanoparticles, as a carrier for the delivery of IKBKB genes silencer, were used because they have shown potential to enhance microglial targeting. SNL rats were injected with IKBKB siRNA-encapsulated PLGA nanoparticles intrathecally for behavioral tests on pain response. IKBKB siRNA was delivered for suppressing the expression of IKBKB. In rats injected with IKBKB siRNA-encapsulated PLGA nanoparticles, allodynia caused by mechanical stimulation was reduced, and the secretion of pro-inflammatory mediators due to NF-κB was reduced. Delivering IKBKB siRNA through PLGA nanoparticles can effectively control the inflammatory response and is worth studying as a treatment for neuropathic pain. MDPI 2021-05-26 /pmc/articles/PMC8203094/ /pubmed/34073390 http://dx.doi.org/10.3390/ijms22115657 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Seounghun
Shin, Hyo-Jung
Noh, Chan
Kim, Song-I
Ko, Young-Kwon
Lee, Sun-Yeul
Lim, Chaeseong
Hong, Boohwi
Yang, Sin-Young
Kim, Dong-Woon
Lee, Won-Hyung
Kim, Yoon-Hee
IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
title IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
title_full IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
title_fullStr IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
title_full_unstemmed IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
title_short IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
title_sort ikbkb sirna-encapsulated poly (lactic-co-glycolic acid) nanoparticles diminish neuropathic pain by inhibiting microglial activation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203094/
https://www.ncbi.nlm.nih.gov/pubmed/34073390
http://dx.doi.org/10.3390/ijms22115657
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