Cargando…
IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation
Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to brea...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203094/ https://www.ncbi.nlm.nih.gov/pubmed/34073390 http://dx.doi.org/10.3390/ijms22115657 |
_version_ | 1783708096135168000 |
---|---|
author | Lee, Seounghun Shin, Hyo-Jung Noh, Chan Kim, Song-I Ko, Young-Kwon Lee, Sun-Yeul Lim, Chaeseong Hong, Boohwi Yang, Sin-Young Kim, Dong-Woon Lee, Won-Hyung Kim, Yoon-Hee |
author_facet | Lee, Seounghun Shin, Hyo-Jung Noh, Chan Kim, Song-I Ko, Young-Kwon Lee, Sun-Yeul Lim, Chaeseong Hong, Boohwi Yang, Sin-Young Kim, Dong-Woon Lee, Won-Hyung Kim, Yoon-Hee |
author_sort | Lee, Seounghun |
collection | PubMed |
description | Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to break down IκB, which blocks the activity of NF-kB. Therefore, it prevents the activity of NK-kB. This study investigated whether neuropathic pain can be reduced in spinal nerve ligation (SNL) rats by reducing the activity of microglia by delivering IKBKB small interfering RNA (siRNA)-encapsulated poly (lactic-co-glycolic acid) (PLGA) nanoparticles. PLGA nanoparticles, as a carrier for the delivery of IKBKB genes silencer, were used because they have shown potential to enhance microglial targeting. SNL rats were injected with IKBKB siRNA-encapsulated PLGA nanoparticles intrathecally for behavioral tests on pain response. IKBKB siRNA was delivered for suppressing the expression of IKBKB. In rats injected with IKBKB siRNA-encapsulated PLGA nanoparticles, allodynia caused by mechanical stimulation was reduced, and the secretion of pro-inflammatory mediators due to NF-κB was reduced. Delivering IKBKB siRNA through PLGA nanoparticles can effectively control the inflammatory response and is worth studying as a treatment for neuropathic pain. |
format | Online Article Text |
id | pubmed-8203094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82030942021-06-15 IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation Lee, Seounghun Shin, Hyo-Jung Noh, Chan Kim, Song-I Ko, Young-Kwon Lee, Sun-Yeul Lim, Chaeseong Hong, Boohwi Yang, Sin-Young Kim, Dong-Woon Lee, Won-Hyung Kim, Yoon-Hee Int J Mol Sci Article Activation of nuclear factor-kappa B (NF-κB) in microglia plays a decisive role in the progress of neuropathic pain, and the inhibitor of kappa B (IκB) is a protein that blocks the activation of NF-κB and is degraded by the inhibitor of NF-κB kinase subunit beta (IKBKB). The role of IKBKB is to break down IκB, which blocks the activity of NF-kB. Therefore, it prevents the activity of NK-kB. This study investigated whether neuropathic pain can be reduced in spinal nerve ligation (SNL) rats by reducing the activity of microglia by delivering IKBKB small interfering RNA (siRNA)-encapsulated poly (lactic-co-glycolic acid) (PLGA) nanoparticles. PLGA nanoparticles, as a carrier for the delivery of IKBKB genes silencer, were used because they have shown potential to enhance microglial targeting. SNL rats were injected with IKBKB siRNA-encapsulated PLGA nanoparticles intrathecally for behavioral tests on pain response. IKBKB siRNA was delivered for suppressing the expression of IKBKB. In rats injected with IKBKB siRNA-encapsulated PLGA nanoparticles, allodynia caused by mechanical stimulation was reduced, and the secretion of pro-inflammatory mediators due to NF-κB was reduced. Delivering IKBKB siRNA through PLGA nanoparticles can effectively control the inflammatory response and is worth studying as a treatment for neuropathic pain. MDPI 2021-05-26 /pmc/articles/PMC8203094/ /pubmed/34073390 http://dx.doi.org/10.3390/ijms22115657 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Seounghun Shin, Hyo-Jung Noh, Chan Kim, Song-I Ko, Young-Kwon Lee, Sun-Yeul Lim, Chaeseong Hong, Boohwi Yang, Sin-Young Kim, Dong-Woon Lee, Won-Hyung Kim, Yoon-Hee IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation |
title | IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation |
title_full | IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation |
title_fullStr | IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation |
title_full_unstemmed | IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation |
title_short | IKBKB siRNA-Encapsulated Poly (Lactic-co-Glycolic Acid) Nanoparticles Diminish Neuropathic Pain by Inhibiting Microglial Activation |
title_sort | ikbkb sirna-encapsulated poly (lactic-co-glycolic acid) nanoparticles diminish neuropathic pain by inhibiting microglial activation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203094/ https://www.ncbi.nlm.nih.gov/pubmed/34073390 http://dx.doi.org/10.3390/ijms22115657 |
work_keys_str_mv | AT leeseounghun ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT shinhyojung ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT nohchan ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT kimsongi ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT koyoungkwon ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT leesunyeul ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT limchaeseong ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT hongboohwi ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT yangsinyoung ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT kimdongwoon ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT leewonhyung ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation AT kimyoonhee ikbkbsirnaencapsulatedpolylacticcoglycolicacidnanoparticlesdiminishneuropathicpainbyinhibitingmicroglialactivation |