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Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates

BACKGROUND: Given the increased fluconazole resistance (FR) among Candida isolates, we assessed the suitability of disk diffusion susceptibility testing (DDT) for the early detection of FR using well-characterized Candida isolates. METHODS: In total, 188 Candida isolates, including 66 C. albicans (s...

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Autores principales: Jeon, Suhak, Shin, Jong Hee, Lim, Ha Jin, Choi, Min Ji, Byun, Seung A, Lee, Dain, Lee, Seung Yeob, Won, Eun Jeong, Kim, Soo Hyun, Shin, Myung Geun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Laboratory Medicine 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203430/
https://www.ncbi.nlm.nih.gov/pubmed/34108283
http://dx.doi.org/10.3343/alm.2021.41.6.559
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author Jeon, Suhak
Shin, Jong Hee
Lim, Ha Jin
Choi, Min Ji
Byun, Seung A
Lee, Dain
Lee, Seung Yeob
Won, Eun Jeong
Kim, Soo Hyun
Shin, Myung Geun
author_facet Jeon, Suhak
Shin, Jong Hee
Lim, Ha Jin
Choi, Min Ji
Byun, Seung A
Lee, Dain
Lee, Seung Yeob
Won, Eun Jeong
Kim, Soo Hyun
Shin, Myung Geun
author_sort Jeon, Suhak
collection PubMed
description BACKGROUND: Given the increased fluconazole resistance (FR) among Candida isolates, we assessed the suitability of disk diffusion susceptibility testing (DDT) for the early detection of FR using well-characterized Candida isolates. METHODS: In total, 188 Candida isolates, including 66 C. albicans (seven Erg11 mutants), 69 C. glabrata (33 Pdr1 mutants), 29 C. parapsilosis (15 Erg11 mutants), and 24 C. tropicalis (eight Erg11 mutants) isolates, were tested in this study. FR was assessed using DDT according to the standard CLSI M44-ED3 method, except that two cell suspensions, McFarland 0.5 (standard inoculum) and 2.5 (large inoculum), were used, and the inhibition zones were read at 2-hour intervals from 10 hours to 24 hours. RESULTS: DDT results for the standard inoculum were readable after 14 hours (C. albicans, C. glabrata, and C. tropicalis) and 20 hours (C. parapsilosis) for >95% of the isolates, whereas the results for the large inoculum were readable after 12 hours (C. glabrata and C. tropicalis), 14 hours (C. albicans), and 16 hours (C. parapsilosis) for >95% of the isolates. Compared with the results produced using the CLSI M27-ED4 broth microdilution method, the first readable results from the DDT method for each isolate exhibited an agreement of 97.0%, 98.6%, 72.4%, and 91.7% for the standard inoculum and 100%, 98.6%, 96.6%, and 95.8% for the large inoculum for C. albicans, C. glabrata, C. parapsilosis, and C. tropicalis, respectively. CONCLUSIONS: DDT using large inoculum may detect FR rapidly and reliably in the four most common Candida species.
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spelling pubmed-82034302021-11-01 Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates Jeon, Suhak Shin, Jong Hee Lim, Ha Jin Choi, Min Ji Byun, Seung A Lee, Dain Lee, Seung Yeob Won, Eun Jeong Kim, Soo Hyun Shin, Myung Geun Ann Lab Med Original Article BACKGROUND: Given the increased fluconazole resistance (FR) among Candida isolates, we assessed the suitability of disk diffusion susceptibility testing (DDT) for the early detection of FR using well-characterized Candida isolates. METHODS: In total, 188 Candida isolates, including 66 C. albicans (seven Erg11 mutants), 69 C. glabrata (33 Pdr1 mutants), 29 C. parapsilosis (15 Erg11 mutants), and 24 C. tropicalis (eight Erg11 mutants) isolates, were tested in this study. FR was assessed using DDT according to the standard CLSI M44-ED3 method, except that two cell suspensions, McFarland 0.5 (standard inoculum) and 2.5 (large inoculum), were used, and the inhibition zones were read at 2-hour intervals from 10 hours to 24 hours. RESULTS: DDT results for the standard inoculum were readable after 14 hours (C. albicans, C. glabrata, and C. tropicalis) and 20 hours (C. parapsilosis) for >95% of the isolates, whereas the results for the large inoculum were readable after 12 hours (C. glabrata and C. tropicalis), 14 hours (C. albicans), and 16 hours (C. parapsilosis) for >95% of the isolates. Compared with the results produced using the CLSI M27-ED4 broth microdilution method, the first readable results from the DDT method for each isolate exhibited an agreement of 97.0%, 98.6%, 72.4%, and 91.7% for the standard inoculum and 100%, 98.6%, 96.6%, and 95.8% for the large inoculum for C. albicans, C. glabrata, C. parapsilosis, and C. tropicalis, respectively. CONCLUSIONS: DDT using large inoculum may detect FR rapidly and reliably in the four most common Candida species. Korean Society for Laboratory Medicine 2021-11-01 2021-11-01 /pmc/articles/PMC8203430/ /pubmed/34108283 http://dx.doi.org/10.3343/alm.2021.41.6.559 Text en © Korean Society for Laboratory Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jeon, Suhak
Shin, Jong Hee
Lim, Ha Jin
Choi, Min Ji
Byun, Seung A
Lee, Dain
Lee, Seung Yeob
Won, Eun Jeong
Kim, Soo Hyun
Shin, Myung Geun
Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates
title Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates
title_full Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates
title_fullStr Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates
title_full_unstemmed Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates
title_short Disk Diffusion Susceptibility Testing for the Rapid Detection of Fluconazole Resistance in Candida Isolates
title_sort disk diffusion susceptibility testing for the rapid detection of fluconazole resistance in candida isolates
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203430/
https://www.ncbi.nlm.nih.gov/pubmed/34108283
http://dx.doi.org/10.3343/alm.2021.41.6.559
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