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Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes

This study aimed to analyze the interaction of primaquine (PQ), chloroquine (CQ), and liposomes to support the design of optimal liposomal delivery for hepatic stage malaria infectious disease. The liposomes were composed of hydrogenated soybean phosphatidylcholine, cholesterol, and distearoyl-sn-gl...

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Autores principales: Miatmoko, Andang, Nurjannah, Ira, Nehru, Nuril Fadilatul, Rosita, Noorma, Hendradi, Esti, Sari, Retno, Ekowati, Juni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203617/
https://www.ncbi.nlm.nih.gov/pubmed/34127730
http://dx.doi.org/10.1038/s41598-021-91866-0
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author Miatmoko, Andang
Nurjannah, Ira
Nehru, Nuril Fadilatul
Rosita, Noorma
Hendradi, Esti
Sari, Retno
Ekowati, Juni
author_facet Miatmoko, Andang
Nurjannah, Ira
Nehru, Nuril Fadilatul
Rosita, Noorma
Hendradi, Esti
Sari, Retno
Ekowati, Juni
author_sort Miatmoko, Andang
collection PubMed
description This study aimed to analyze the interaction of primaquine (PQ), chloroquine (CQ), and liposomes to support the design of optimal liposomal delivery for hepatic stage malaria infectious disease. The liposomes were composed of hydrogenated soybean phosphatidylcholine, cholesterol, and distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy[polyethyleneglycol]-2000), prepared by thin film method, then evaluated for physicochemical and spectrospic characteristics. The calcein release was further evaluated to determine the effect of drug co-loading on liposomal membrane integrity. The results showed that loading PQ and CQ into liposomes produced changes in the infrared spectra of the diester phosphate and carbonyl ester located in the polar part of the phospholipid, in addition to the alkyl group (CH(2)) in the nonpolar portion. Moreover, the thermogram revealed the loss of the endothermic peak of liposomes dually loaded with PQ and CQ at 186.6 °C, which is identical to that of the phospholipid. However, no crystallinity changes were detected through powder X-ray diffraction analysis. Moreover, PQ, with either single or dual loading, produced the higher calcein release profiles from the liposomes than that of CQ. The dual loading of PQ and CQ tends to interact with the polar head group of the phosphatidylcholine bilayer membrane resulted in an increase in water permeability of the liposomes.
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spelling pubmed-82036172021-06-15 Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes Miatmoko, Andang Nurjannah, Ira Nehru, Nuril Fadilatul Rosita, Noorma Hendradi, Esti Sari, Retno Ekowati, Juni Sci Rep Article This study aimed to analyze the interaction of primaquine (PQ), chloroquine (CQ), and liposomes to support the design of optimal liposomal delivery for hepatic stage malaria infectious disease. The liposomes were composed of hydrogenated soybean phosphatidylcholine, cholesterol, and distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy[polyethyleneglycol]-2000), prepared by thin film method, then evaluated for physicochemical and spectrospic characteristics. The calcein release was further evaluated to determine the effect of drug co-loading on liposomal membrane integrity. The results showed that loading PQ and CQ into liposomes produced changes in the infrared spectra of the diester phosphate and carbonyl ester located in the polar part of the phospholipid, in addition to the alkyl group (CH(2)) in the nonpolar portion. Moreover, the thermogram revealed the loss of the endothermic peak of liposomes dually loaded with PQ and CQ at 186.6 °C, which is identical to that of the phospholipid. However, no crystallinity changes were detected through powder X-ray diffraction analysis. Moreover, PQ, with either single or dual loading, produced the higher calcein release profiles from the liposomes than that of CQ. The dual loading of PQ and CQ tends to interact with the polar head group of the phosphatidylcholine bilayer membrane resulted in an increase in water permeability of the liposomes. Nature Publishing Group UK 2021-06-14 /pmc/articles/PMC8203617/ /pubmed/34127730 http://dx.doi.org/10.1038/s41598-021-91866-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Miatmoko, Andang
Nurjannah, Ira
Nehru, Nuril Fadilatul
Rosita, Noorma
Hendradi, Esti
Sari, Retno
Ekowati, Juni
Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes
title Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes
title_full Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes
title_fullStr Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes
title_full_unstemmed Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes
title_short Interactions of primaquine and chloroquine with PEGylated phosphatidylcholine liposomes
title_sort interactions of primaquine and chloroquine with pegylated phosphatidylcholine liposomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203617/
https://www.ncbi.nlm.nih.gov/pubmed/34127730
http://dx.doi.org/10.1038/s41598-021-91866-0
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