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Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research
For research on tendon injury, many different animal models are utilized; however, the extent to which these species simulate the clinical condition and disease pathophysiology has not yet been critically evaluated. Considering the importance of inflammation in tendon disease, this study compared th...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203623/ https://www.ncbi.nlm.nih.gov/pubmed/34127759 http://dx.doi.org/10.1038/s41598-021-91914-9 |
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author | Oreff, Gil Lola Fenu, Michele Vogl, Claus Ribitsch, Iris Jenner, Florien |
author_facet | Oreff, Gil Lola Fenu, Michele Vogl, Claus Ribitsch, Iris Jenner, Florien |
author_sort | Oreff, Gil Lola |
collection | PubMed |
description | For research on tendon injury, many different animal models are utilized; however, the extent to which these species simulate the clinical condition and disease pathophysiology has not yet been critically evaluated. Considering the importance of inflammation in tendon disease, this study compared the cellular and molecular features of inflammation in tenocytes of humans and four common model species (mouse, rat, sheep, and horse). While mouse and rat tenocytes most closely equalled human tenocytes’ low proliferation capacity and the negligible effect of inflammation on proliferation, the wound closure speed of humans was best approximated by rats and horses. The overall gene expression of human tenocytes was most similar to mice under healthy, to horses under transient and to sheep under constant inflammatory conditions. Humans were best matched by mice and horses in their tendon marker and collagen expression, by horses in extracellular matrix remodelling genes, and by rats in inflammatory mediators. As no single animal model perfectly replicates the clinical condition and sufficiently emulates human tenocytes, fit-for-purpose selection of the model species for each specific research question and combination of data from multiple species will be essential to optimize translational predictive validity. |
format | Online Article Text |
id | pubmed-8203623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-82036232021-06-15 Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research Oreff, Gil Lola Fenu, Michele Vogl, Claus Ribitsch, Iris Jenner, Florien Sci Rep Article For research on tendon injury, many different animal models are utilized; however, the extent to which these species simulate the clinical condition and disease pathophysiology has not yet been critically evaluated. Considering the importance of inflammation in tendon disease, this study compared the cellular and molecular features of inflammation in tenocytes of humans and four common model species (mouse, rat, sheep, and horse). While mouse and rat tenocytes most closely equalled human tenocytes’ low proliferation capacity and the negligible effect of inflammation on proliferation, the wound closure speed of humans was best approximated by rats and horses. The overall gene expression of human tenocytes was most similar to mice under healthy, to horses under transient and to sheep under constant inflammatory conditions. Humans were best matched by mice and horses in their tendon marker and collagen expression, by horses in extracellular matrix remodelling genes, and by rats in inflammatory mediators. As no single animal model perfectly replicates the clinical condition and sufficiently emulates human tenocytes, fit-for-purpose selection of the model species for each specific research question and combination of data from multiple species will be essential to optimize translational predictive validity. Nature Publishing Group UK 2021-06-14 /pmc/articles/PMC8203623/ /pubmed/34127759 http://dx.doi.org/10.1038/s41598-021-91914-9 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Oreff, Gil Lola Fenu, Michele Vogl, Claus Ribitsch, Iris Jenner, Florien Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research |
title | Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research |
title_full | Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research |
title_fullStr | Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research |
title_full_unstemmed | Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research |
title_short | Species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research |
title_sort | species variations in tenocytes’ response to inflammation require careful selection of animal models for tendon research |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203623/ https://www.ncbi.nlm.nih.gov/pubmed/34127759 http://dx.doi.org/10.1038/s41598-021-91914-9 |
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