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Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots

Root metaxylem vessels are responsible for axial water transport and contribute to hydraulic architecture. Variation in metaxylem vessel size and number can impact drought tolerance in crop plants, including rice, a crop that is particularly sensitive to drought. Identifying and validating candidate...

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Autores principales: Reeger, Jenna E., Wheatley, Matthew, Yang, Yinong, Brown, Kathleen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204146/
https://www.ncbi.nlm.nih.gov/pubmed/34142002
http://dx.doi.org/10.1002/pld3.328
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author Reeger, Jenna E.
Wheatley, Matthew
Yang, Yinong
Brown, Kathleen M.
author_facet Reeger, Jenna E.
Wheatley, Matthew
Yang, Yinong
Brown, Kathleen M.
author_sort Reeger, Jenna E.
collection PubMed
description Root metaxylem vessels are responsible for axial water transport and contribute to hydraulic architecture. Variation in metaxylem vessel size and number can impact drought tolerance in crop plants, including rice, a crop that is particularly sensitive to drought. Identifying and validating candidate genes for metaxylem development would aid breeding efforts for improved varieties for drought tolerance. We identified three transcription factor candidate genes that potentially regulate metaxylem vessel size and number in rice based on orthologous annotations, published expression data, and available root and drought‐related QTL data. Single gene knockout mutants were generated for each candidate using CRISPR‐Cas9 genome editing. Root metaxylem vessel area and number were analyzed in 6‐week‐old knockout mutants and wild‐type plants under well‐watered and drought conditions in the greenhouse. Compared with wild type, LONESOME HIGHWAY (OsLHW) mutants had fewer, smaller metaxylem vessels in shallow roots and more, larger vessels in deep roots in drought conditions, indicating that OsLHW may be a repressor of drought‐induced metaxylem plasticity. The AUXIN RESPONSE FACTOR 15 mutants showed fewer but larger metaxylem vessel area in well‐watered conditions, but phenotypes were inconsistent under drought treatment. ORYZA SATIVA HOMEBOX 6 (OSH6) mutants had fewer, smaller metaxylem vessels in well‐watered conditions with greater effects on xylem number than size. OSH6 mutants had larger shoots and more, deeper roots than the wild type in well‐watered conditions, but there were no differences in performance under drought between mutants and wild type. Though these candidate gene mutants did not exhibit large phenotypic effects, the identification and investigation of candidate genes related to metaxylem traits in rice deepen our understanding of metaxylem development and are needed to facilitate incorporation of favorable alleles into breeding populations to improve drought stress tolerance.
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spelling pubmed-82041462021-06-16 Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots Reeger, Jenna E. Wheatley, Matthew Yang, Yinong Brown, Kathleen M. Plant Direct Original Research Root metaxylem vessels are responsible for axial water transport and contribute to hydraulic architecture. Variation in metaxylem vessel size and number can impact drought tolerance in crop plants, including rice, a crop that is particularly sensitive to drought. Identifying and validating candidate genes for metaxylem development would aid breeding efforts for improved varieties for drought tolerance. We identified three transcription factor candidate genes that potentially regulate metaxylem vessel size and number in rice based on orthologous annotations, published expression data, and available root and drought‐related QTL data. Single gene knockout mutants were generated for each candidate using CRISPR‐Cas9 genome editing. Root metaxylem vessel area and number were analyzed in 6‐week‐old knockout mutants and wild‐type plants under well‐watered and drought conditions in the greenhouse. Compared with wild type, LONESOME HIGHWAY (OsLHW) mutants had fewer, smaller metaxylem vessels in shallow roots and more, larger vessels in deep roots in drought conditions, indicating that OsLHW may be a repressor of drought‐induced metaxylem plasticity. The AUXIN RESPONSE FACTOR 15 mutants showed fewer but larger metaxylem vessel area in well‐watered conditions, but phenotypes were inconsistent under drought treatment. ORYZA SATIVA HOMEBOX 6 (OSH6) mutants had fewer, smaller metaxylem vessels in well‐watered conditions with greater effects on xylem number than size. OSH6 mutants had larger shoots and more, deeper roots than the wild type in well‐watered conditions, but there were no differences in performance under drought between mutants and wild type. Though these candidate gene mutants did not exhibit large phenotypic effects, the identification and investigation of candidate genes related to metaxylem traits in rice deepen our understanding of metaxylem development and are needed to facilitate incorporation of favorable alleles into breeding populations to improve drought stress tolerance. John Wiley and Sons Inc. 2021-06-15 /pmc/articles/PMC8204146/ /pubmed/34142002 http://dx.doi.org/10.1002/pld3.328 Text en © 2021 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Research
Reeger, Jenna E.
Wheatley, Matthew
Yang, Yinong
Brown, Kathleen M.
Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots
title Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots
title_full Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots
title_fullStr Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots
title_full_unstemmed Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots
title_short Targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots
title_sort targeted mutation of transcription factor genes alters metaxylem vessel size and number in rice roots
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204146/
https://www.ncbi.nlm.nih.gov/pubmed/34142002
http://dx.doi.org/10.1002/pld3.328
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