Cargando…

Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts

BACKGROUND: In mammals, the ovary is the essential system of female reproduction for the onset of puberty, and the abnormal puberty has negative outcomes on health. CircRNA is a non-coding RNA produced by non-canonical alternative splicing (AS). Several studies have reported that circRNA is involved...

Descripción completa

Detalles Bibliográficos
Autores principales: Pan, Xiangchun, Gong, Wentao, He, Yingting, Li, Nian, Zhang, Hao, Zhang, Zhe, Li, Jiaqi, Yuan, Xiaolong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204460/
https://www.ncbi.nlm.nih.gov/pubmed/34126925
http://dx.doi.org/10.1186/s12864-021-07786-w
_version_ 1783708344445304832
author Pan, Xiangchun
Gong, Wentao
He, Yingting
Li, Nian
Zhang, Hao
Zhang, Zhe
Li, Jiaqi
Yuan, Xiaolong
author_facet Pan, Xiangchun
Gong, Wentao
He, Yingting
Li, Nian
Zhang, Hao
Zhang, Zhe
Li, Jiaqi
Yuan, Xiaolong
author_sort Pan, Xiangchun
collection PubMed
description BACKGROUND: In mammals, the ovary is the essential system of female reproduction for the onset of puberty, and the abnormal puberty has negative outcomes on health. CircRNA is a non-coding RNA produced by non-canonical alternative splicing (AS). Several studies have reported that circRNA is involved in the gene regulation and plays an important role in some human diseases. However, the contribution of circRNA has received little known within the onset of puberty in ovary. RESULTS: Here, the profiles of ovarian circRNAs across pre-, in- and post-pubertal stages were established by RNA-sEq. In total, 972 circRNAs were identified, including 631 stage-specific circRNAs and 8 tissue-specific circRNAs. The biological functions of parental genes of circRNAs were enriched in steroid biosynthesis, autophagy-animal, MAPK signaling pathway, progesterone-mediated oocyte maturation and ras signaling pathway. Moreover, 5 circRNAs derived from 4 puberty-related genes (ESR1, JAK2, NF1 and ARNT) were found in this study. The A3SS events were the most alternative splicing, but IR events were likely to be arose in post-pubertal ovaries. Besides, the circRNA-miRNA-gene networks were explored for 10 differentially expressed circRNAs. Furthermore, the head-to-tail exon as well as the expressions of 10 circRNAs were validated by the divergent RT-qPCR and sanger sequencing. CONCLUSIONS: In summary, the profiles of ovarian circRNAs were provided during pubertal transition in gilts, and these results provided useful information for the investigation on the onset of puberty at the ovarian-circRNAs-level in mammals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07786-w.
format Online
Article
Text
id pubmed-8204460
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-82044602021-06-16 Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts Pan, Xiangchun Gong, Wentao He, Yingting Li, Nian Zhang, Hao Zhang, Zhe Li, Jiaqi Yuan, Xiaolong BMC Genomics Research BACKGROUND: In mammals, the ovary is the essential system of female reproduction for the onset of puberty, and the abnormal puberty has negative outcomes on health. CircRNA is a non-coding RNA produced by non-canonical alternative splicing (AS). Several studies have reported that circRNA is involved in the gene regulation and plays an important role in some human diseases. However, the contribution of circRNA has received little known within the onset of puberty in ovary. RESULTS: Here, the profiles of ovarian circRNAs across pre-, in- and post-pubertal stages were established by RNA-sEq. In total, 972 circRNAs were identified, including 631 stage-specific circRNAs and 8 tissue-specific circRNAs. The biological functions of parental genes of circRNAs were enriched in steroid biosynthesis, autophagy-animal, MAPK signaling pathway, progesterone-mediated oocyte maturation and ras signaling pathway. Moreover, 5 circRNAs derived from 4 puberty-related genes (ESR1, JAK2, NF1 and ARNT) were found in this study. The A3SS events were the most alternative splicing, but IR events were likely to be arose in post-pubertal ovaries. Besides, the circRNA-miRNA-gene networks were explored for 10 differentially expressed circRNAs. Furthermore, the head-to-tail exon as well as the expressions of 10 circRNAs were validated by the divergent RT-qPCR and sanger sequencing. CONCLUSIONS: In summary, the profiles of ovarian circRNAs were provided during pubertal transition in gilts, and these results provided useful information for the investigation on the onset of puberty at the ovarian-circRNAs-level in mammals. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07786-w. BioMed Central 2021-06-15 /pmc/articles/PMC8204460/ /pubmed/34126925 http://dx.doi.org/10.1186/s12864-021-07786-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Pan, Xiangchun
Gong, Wentao
He, Yingting
Li, Nian
Zhang, Hao
Zhang, Zhe
Li, Jiaqi
Yuan, Xiaolong
Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts
title Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts
title_full Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts
title_fullStr Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts
title_full_unstemmed Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts
title_short Ovary-derived circular RNAs profile analysis during the onset of puberty in gilts
title_sort ovary-derived circular rnas profile analysis during the onset of puberty in gilts
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204460/
https://www.ncbi.nlm.nih.gov/pubmed/34126925
http://dx.doi.org/10.1186/s12864-021-07786-w
work_keys_str_mv AT panxiangchun ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts
AT gongwentao ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts
AT heyingting ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts
AT linian ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts
AT zhanghao ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts
AT zhangzhe ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts
AT lijiaqi ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts
AT yuanxiaolong ovaryderivedcircularrnasprofileanalysisduringtheonsetofpubertyingilts