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Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats

BACKGROUND: Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia. It is well known that diabetes leads to intestinal microbiota dysbiosis. It is also recently discovered that liver glycogen structure is impaired in diabetic animals. Since mulberry leav...

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Autores principales: Liu, Zhan-Zhong, Liu, Qing-Hua, Liu, Zhao, Tang, Jia-Wei, Chua, Eng-Guan, Li, Fen, Xiong, Xue-Song, Wang, Meng-Meng, Wen, Peng-Bo, Shi, Xin-Yi, Xi, Xiang-Yu, Zhang, Xiao, Wang, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204513/
https://www.ncbi.nlm.nih.gov/pubmed/34126977
http://dx.doi.org/10.1186/s12906-021-03342-x
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author Liu, Zhan-Zhong
Liu, Qing-Hua
Liu, Zhao
Tang, Jia-Wei
Chua, Eng-Guan
Li, Fen
Xiong, Xue-Song
Wang, Meng-Meng
Wen, Peng-Bo
Shi, Xin-Yi
Xi, Xiang-Yu
Zhang, Xiao
Wang, Liang
author_facet Liu, Zhan-Zhong
Liu, Qing-Hua
Liu, Zhao
Tang, Jia-Wei
Chua, Eng-Guan
Li, Fen
Xiong, Xue-Song
Wang, Meng-Meng
Wen, Peng-Bo
Shi, Xin-Yi
Xi, Xiang-Yu
Zhang, Xiao
Wang, Liang
author_sort Liu, Zhan-Zhong
collection PubMed
description BACKGROUND: Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia. It is well known that diabetes leads to intestinal microbiota dysbiosis. It is also recently discovered that liver glycogen structure is impaired in diabetic animals. Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure. METHODS: In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day). Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs. Liver glycogen structure was characterized through size exclusion chromatography (SEC). Both Student’s t-test and Tukey’s test were used for statistical analysis. RESULTS: A group of type 2 diabetic rat models were successfully established. Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions. In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves. CONCLUSIONS: This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats. These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-021-03342-x.
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spelling pubmed-82045132021-06-16 Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats Liu, Zhan-Zhong Liu, Qing-Hua Liu, Zhao Tang, Jia-Wei Chua, Eng-Guan Li, Fen Xiong, Xue-Song Wang, Meng-Meng Wen, Peng-Bo Shi, Xin-Yi Xi, Xiang-Yu Zhang, Xiao Wang, Liang BMC Complement Med Ther Research Article BACKGROUND: Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia. It is well known that diabetes leads to intestinal microbiota dysbiosis. It is also recently discovered that liver glycogen structure is impaired in diabetic animals. Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure. METHODS: In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day). Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs. Liver glycogen structure was characterized through size exclusion chromatography (SEC). Both Student’s t-test and Tukey’s test were used for statistical analysis. RESULTS: A group of type 2 diabetic rat models were successfully established. Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions. In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves. CONCLUSIONS: This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats. These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12906-021-03342-x. BioMed Central 2021-06-14 /pmc/articles/PMC8204513/ /pubmed/34126977 http://dx.doi.org/10.1186/s12906-021-03342-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Liu, Zhan-Zhong
Liu, Qing-Hua
Liu, Zhao
Tang, Jia-Wei
Chua, Eng-Guan
Li, Fen
Xiong, Xue-Song
Wang, Meng-Meng
Wen, Peng-Bo
Shi, Xin-Yi
Xi, Xiang-Yu
Zhang, Xiao
Wang, Liang
Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
title Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
title_full Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
title_fullStr Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
title_full_unstemmed Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
title_short Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
title_sort ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204513/
https://www.ncbi.nlm.nih.gov/pubmed/34126977
http://dx.doi.org/10.1186/s12906-021-03342-x
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