Cargando…

Transcribing Genes the Hard Way: In Vitro Reconstitution of Nanoarchaeal RNA Polymerase Reveals Unusual Active Site Properties

Nanoarchaea represent a highly diverged archaeal phylum that displays many unusual biological features. The Nanoarchaeum equitans genome encodes a complete set of RNA polymerase (RNAP) subunits and basal factors. Several of the standard motifs in the active center contain radical substitutions that...

Descripción completa

Detalles Bibliográficos
Autores principales:	Nottebaum, Sven, Weinzierl, Robert O. J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Molecular Biosciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204694/ https://www.ncbi.nlm.nih.gov/pubmed/34141723 http://dx.doi.org/10.3389/fmolb.2021.669314

Ejemplares similares

Eukaryotic RNA Polymerases: The Many Ways to Transcribe a Gene
por: Barba-Aliaga, Marina, et al.
Publicado: (2021)

Translation of in vitro-transcribed RNA therapeutics
por: von der Haar, Tobias, et al.
Publicado: (2023)

Reconstitution of Pure Chaperonin Hetero-Oligomer Preparations in Vitro by Temperature Modulation
por: Vitlin Gruber, Anna, et al.
Publicado: (2018)

Functionality of In vitro Reconstituted Group II Intron RmInt1-Derived Ribonucleoprotein Particles
por: Molina-Sánchez, Maria D., et al.
Publicado: (2016)

Divergently Transcribed ncRNAs in Escherichia coli: Refinement of the Transcription Starts Assumes Functional Diversification
por: Kiselev, Sergey, et al.
Publicado: (2021)

Efficient and stable reconstitution of the ABC transporter BmrA for solid-state NMR studies
por: Kunert, Britta, et al.
Publicado: (2014)

Reconstitution and NMR Characterization of the Ion-Channel Accessory Subunit Barttin in Detergents and Lipid-Bilayer Nanodiscs
por: Viennet, Thibault, et al.
Publicado: (2019)

Editorial: From reconstituting minimal cell-cell signaling systems to bioinspired synthetic communication networks
por: Ganzinger, Kristina A., et al.
Publicado: (2022)

Biogenesis of RNA Polymerases in Yeast
por: Garrido-Godino, Ana I., et al.
Publicado: (2021)

The RNA polymerase factory: a robotic in vitro assembly platform for high-throughput production of recombinant protein complexes
por: Nottebaum, Sven, et al.
Publicado: (2008)

Structural and electronic properties of the active site of [ZnFe] SulE
por: Moubarak, Samah, et al.
Publicado: (2022)

Editorial: The Lesser Known World of RNA Polymerases
por: Calvo, Olga, et al.
Publicado: (2021)

Computational Ways to Enhance Protein Inhibitor Design
por: Jernigan, Robert L., et al.
Publicado: (2021)

Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
por: Gardner, Andrew F., et al.
Publicado: (2019)

RNA Polymerase III Subunit Mutations in Genetic Diseases
por: Lata, Elisabeth, et al.
Publicado: (2021)

Phosphorylation Induced Conformational Transitions in DNA Polymerase β
por: Srivastava, Amit, et al.
Publicado: (2022)

Paving the way for precise diagnostics of antimicrobial resistant bacteria
por: Wang, Hao, et al.
Publicado: (2022)

In vitro DNA Inversions Mediated by the PsrA Site-Specific Tyrosine Recombinase of Streptococcus pneumoniae
por: Li, Jingwen, et al.
Publicado: (2020)

Inhibiting DNA Polymerases as a Therapeutic Intervention against Cancer
por: Berdis, Anthony J.
Publicado: (2017)

When DNA Polymerases Multitask: Functions Beyond Nucleotidyl Transfer
por: Carvajal-Maldonado, Denisse, et al.
Publicado: (2022)

Specific Features of RNA Polymerases I and III: Structure and Assembly
por: Turowski, Tomasz W., et al.
Publicado: (2021)

Conformational Dynamics of DNA Polymerases Revealed at the Single-Molecule Level
por: Millar, David P.
Publicado: (2022)

Coupling Between Cell Cycle Progression and the Nuclear RNA Polymerases System
por: Delgado-Román, Irene, et al.
Publicado: (2021)

Editorial: Nucleic Acid Polymerases: The Two-Metal-Ion Mechanism and Beyond
por: Pata, Janice D., et al.
Publicado: (2022)

Within and Beyond the Nucleotide Addition Cycle of Viral RNA-dependent RNA Polymerases
por: Gong, Peng
Publicado: (2022)

Human Mitochondrial DNA Polymerase Metal Dependent UV Lesion Bypassing Ability
por: Park, Joon, et al.
Publicado: (2022)

Aspirin Repurposing in Folate-Decorated Nanoparticles: Another Way to Target Breast Cancer
por: Kanwal, Fariha, et al.
Publicado: (2022)

Conformational selection and induced fit for RNA polymerase and RNA/DNA hybrid backtracked recognition
por: Wu, Jian, et al.
Publicado: (2015)

Structure of New Binary and Ternary DNA Polymerase Complexes From Bacteriophage RB69
por: Park, Jongseo, et al.
Publicado: (2021)

Regulation of RNA Polymerase II Transcription Initiation and Elongation by Transcription Factor TFII-I
por: Linzer, Niko, et al.
Publicado: (2021)

RNA polymerase III transcription and cancer: A tale of two RPC7 subunits
por: Cheng, Ruiying, et al.
Publicado: (2023)

Alzheimer Cells on Their Way to Derailment Show Selective Changes in Protein Quality Control Network
por: Koopman, Margreet B., et al.
Publicado: (2020)

DNA Polymerase Conformational Dynamics and the Role of Fidelity-Conferring Residues: Insights from Computational Simulations
por: Meli, Massimiliano, et al.
Publicado: (2016)

Bacterial RNA Polymerase-DNA Interaction—The Driving Force of Gene Expression and the Target for Drug Action
por: Lee, Jookyung, et al.
Publicado: (2016)

Two Family B DNA Polymerases From Aeropyrum pernix, Based on Revised Translational Frames
por: Daimon, Katsuya, et al.
Publicado: (2018)

Development of a Polymerase Spiral Reaction-Based Isothermal Assay for Rapid Identification of Thrips palmi
por: Jangra, Sumit, et al.
Publicado: (2022)

Two-Metal-Ion Catalysis: Inhibition of DNA Polymerase Activity by a Third Divalent Metal Ion
por: Wang, Jimin, et al.
Publicado: (2022)

Mobile genetic element-encoded putative DNA primases composed of A-family polymerase—SSB pairs
por: Rice, Phoebe A.
Publicado: (2023)

Modeling nonsegmented negative-strand RNA virus (NNSV) transcription with ejective polymerase collisions and biased diffusion
por: Piedra, Felipe-Andrés, et al.
Publicado: (2023)

Several Isoforms for Each Subunit Shared by RNA Polymerases are Differentially Expressed in the Cultivated Olive Tree (Olea europaea L.)
por: Fernández-Parras, Isabel, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_rii62llsbgqvam15bts9m6i7cj