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The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli
Urinary tract infections (UTIs) are a common bacterial infectious disease in humans, and strains of uropathogenic Escherichia coli (UPEC) are the most frequent cause of UTIs. During infection, UPEC must cope with a variety of stressful conditions in the urinary tract. Here, we demonstrate that the s...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205139/ https://www.ncbi.nlm.nih.gov/pubmed/34043736 http://dx.doi.org/10.1371/journal.ppat.1009617 |
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author | Bessaiah, Hicham Pokharel, Pravil Loucif, Hamza Kulbay, Merve Sasseville, Charles Habouria, Hajer Houle, Sébastien Bernier, Jacques Massé, Éric Van Grevenynghe, Julien Dozois, Charles M. |
author_facet | Bessaiah, Hicham Pokharel, Pravil Loucif, Hamza Kulbay, Merve Sasseville, Charles Habouria, Hajer Houle, Sébastien Bernier, Jacques Massé, Éric Van Grevenynghe, Julien Dozois, Charles M. |
author_sort | Bessaiah, Hicham |
collection | PubMed |
description | Urinary tract infections (UTIs) are a common bacterial infectious disease in humans, and strains of uropathogenic Escherichia coli (UPEC) are the most frequent cause of UTIs. During infection, UPEC must cope with a variety of stressful conditions in the urinary tract. Here, we demonstrate that the small RNA (sRNA) RyfA of UPEC strains is required for resistance to oxidative and osmotic stresses. Transcriptomic analysis of the ryfA mutant showed changes in expression of genes associated with general stress responses, metabolism, biofilm formation and genes coding for cell surface proteins. Inactivation of ryfA in UPEC strain CFT073 decreased urinary tract colonization in mice and the ryfA mutant also had reduced production of type 1 and P fimbriae (pili), adhesins which are known to be important for UTI. Furthermore, loss of ryfA also reduced UPEC survival in human macrophages. Thus, ryfA plays a key regulatory role in UPEC adaptation to stress, which contributes to UTI and survival in macrophages. |
format | Online Article Text |
id | pubmed-8205139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-82051392021-06-29 The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli Bessaiah, Hicham Pokharel, Pravil Loucif, Hamza Kulbay, Merve Sasseville, Charles Habouria, Hajer Houle, Sébastien Bernier, Jacques Massé, Éric Van Grevenynghe, Julien Dozois, Charles M. PLoS Pathog Research Article Urinary tract infections (UTIs) are a common bacterial infectious disease in humans, and strains of uropathogenic Escherichia coli (UPEC) are the most frequent cause of UTIs. During infection, UPEC must cope with a variety of stressful conditions in the urinary tract. Here, we demonstrate that the small RNA (sRNA) RyfA of UPEC strains is required for resistance to oxidative and osmotic stresses. Transcriptomic analysis of the ryfA mutant showed changes in expression of genes associated with general stress responses, metabolism, biofilm formation and genes coding for cell surface proteins. Inactivation of ryfA in UPEC strain CFT073 decreased urinary tract colonization in mice and the ryfA mutant also had reduced production of type 1 and P fimbriae (pili), adhesins which are known to be important for UTI. Furthermore, loss of ryfA also reduced UPEC survival in human macrophages. Thus, ryfA plays a key regulatory role in UPEC adaptation to stress, which contributes to UTI and survival in macrophages. Public Library of Science 2021-05-27 /pmc/articles/PMC8205139/ /pubmed/34043736 http://dx.doi.org/10.1371/journal.ppat.1009617 Text en © 2021 Bessaiah et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bessaiah, Hicham Pokharel, Pravil Loucif, Hamza Kulbay, Merve Sasseville, Charles Habouria, Hajer Houle, Sébastien Bernier, Jacques Massé, Éric Van Grevenynghe, Julien Dozois, Charles M. The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli |
title | The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli |
title_full | The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli |
title_fullStr | The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli |
title_full_unstemmed | The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli |
title_short | The RyfA small RNA regulates oxidative and osmotic stress responses and virulence in uropathogenic Escherichia coli |
title_sort | ryfa small rna regulates oxidative and osmotic stress responses and virulence in uropathogenic escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205139/ https://www.ncbi.nlm.nih.gov/pubmed/34043736 http://dx.doi.org/10.1371/journal.ppat.1009617 |
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