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Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group
Severe Perkinsea infection is an emerging disease of amphibians, specifically tadpoles. Disease presentation correlates with liver infections of a subclade of Perkinsea (Alveolata) protists, named Pathogenic Perkinsea Clade (PPC). Tadpole mortality events associated with PPC infections have been rep...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205526/ https://www.ncbi.nlm.nih.gov/pubmed/34129800 http://dx.doi.org/10.1098/rsbl.2021.0166 |
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author | Smilansky, Vanessa Jirků, Miloslav Milner, David S. Ibáñez, Roberto Gratwicke, Brian Nicholls, Andrew Lukeš, Julius Chambouvet, Aurélie Richards, Thomas A. |
author_facet | Smilansky, Vanessa Jirků, Miloslav Milner, David S. Ibáñez, Roberto Gratwicke, Brian Nicholls, Andrew Lukeš, Julius Chambouvet, Aurélie Richards, Thomas A. |
author_sort | Smilansky, Vanessa |
collection | PubMed |
description | Severe Perkinsea infection is an emerging disease of amphibians, specifically tadpoles. Disease presentation correlates with liver infections of a subclade of Perkinsea (Alveolata) protists, named Pathogenic Perkinsea Clade (PPC). Tadpole mortality events associated with PPC infections have been reported across North America, from Alaska to Florida. Here, we investigate the geographic and host range of PPC associations in seemingly healthy tadpoles sampled from Panama, a biogeographic provenance critically affected by amphibian decline. To complement this work, we also investigate a mortality event among Hyla arborea tadpoles in captive-bred UK specimens. PPC SSU rDNA was detected in 10 of 81 Panama tadpoles tested, and H. arborea tadpoles from the UK. Phylogenies of the Perkinsea SSU rDNA sequences demonstrate they are highly similar to PPC sequences sampled from mortality events in the USA, and phylogenetic analysis of tadpole mitochondrial SSU rDNA demonstrates, for the first time, PPC associations in diverse hylids. These data provide further understanding of the biogeography and host range of this putative pathogenic group, factors likely to be important for conservation planning. |
format | Online Article Text |
id | pubmed-8205526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-82055262021-06-16 Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group Smilansky, Vanessa Jirků, Miloslav Milner, David S. Ibáñez, Roberto Gratwicke, Brian Nicholls, Andrew Lukeš, Julius Chambouvet, Aurélie Richards, Thomas A. Biol Lett Conservation Biology Severe Perkinsea infection is an emerging disease of amphibians, specifically tadpoles. Disease presentation correlates with liver infections of a subclade of Perkinsea (Alveolata) protists, named Pathogenic Perkinsea Clade (PPC). Tadpole mortality events associated with PPC infections have been reported across North America, from Alaska to Florida. Here, we investigate the geographic and host range of PPC associations in seemingly healthy tadpoles sampled from Panama, a biogeographic provenance critically affected by amphibian decline. To complement this work, we also investigate a mortality event among Hyla arborea tadpoles in captive-bred UK specimens. PPC SSU rDNA was detected in 10 of 81 Panama tadpoles tested, and H. arborea tadpoles from the UK. Phylogenies of the Perkinsea SSU rDNA sequences demonstrate they are highly similar to PPC sequences sampled from mortality events in the USA, and phylogenetic analysis of tadpole mitochondrial SSU rDNA demonstrates, for the first time, PPC associations in diverse hylids. These data provide further understanding of the biogeography and host range of this putative pathogenic group, factors likely to be important for conservation planning. The Royal Society 2021-06-16 /pmc/articles/PMC8205526/ /pubmed/34129800 http://dx.doi.org/10.1098/rsbl.2021.0166 Text en © 2021 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Conservation Biology Smilansky, Vanessa Jirků, Miloslav Milner, David S. Ibáñez, Roberto Gratwicke, Brian Nicholls, Andrew Lukeš, Julius Chambouvet, Aurélie Richards, Thomas A. Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group |
title | Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group |
title_full | Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group |
title_fullStr | Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group |
title_full_unstemmed | Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group |
title_short | Expanded host and geographic range of tadpole associations with the Severe Perkinsea Infection group |
title_sort | expanded host and geographic range of tadpole associations with the severe perkinsea infection group |
topic | Conservation Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8205526/ https://www.ncbi.nlm.nih.gov/pubmed/34129800 http://dx.doi.org/10.1098/rsbl.2021.0166 |
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