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MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is a common and high-mortality cancer worldwide. Numerous microRNAs have crucial roles in the progression of different cancers. However, identifying the important microRNAs and the target biological function of the microRNA in HCC progression is difficult. In this stud...

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Autores principales: Liu, Yuling, Zhang, Yuanzhou, Xiao, Bowen, Tang, Ning, Hu, Jingying, Liang, Shunshun, Pang, Yechun, Xu, Huili, Ao, Junping, Yang, Juan, Liang, Xiaofei, Wei, Lin, Wang, Yunfeng, Luo, Xiaoying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206076/
https://www.ncbi.nlm.nih.gov/pubmed/34131101
http://dx.doi.org/10.1038/s41419-021-03905-3
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author Liu, Yuling
Zhang, Yuanzhou
Xiao, Bowen
Tang, Ning
Hu, Jingying
Liang, Shunshun
Pang, Yechun
Xu, Huili
Ao, Junping
Yang, Juan
Liang, Xiaofei
Wei, Lin
Wang, Yunfeng
Luo, Xiaoying
author_facet Liu, Yuling
Zhang, Yuanzhou
Xiao, Bowen
Tang, Ning
Hu, Jingying
Liang, Shunshun
Pang, Yechun
Xu, Huili
Ao, Junping
Yang, Juan
Liang, Xiaofei
Wei, Lin
Wang, Yunfeng
Luo, Xiaoying
author_sort Liu, Yuling
collection PubMed
description Hepatocellular carcinoma (HCC) is a common and high-mortality cancer worldwide. Numerous microRNAs have crucial roles in the progression of different cancers. However, identifying the important microRNAs and the target biological function of the microRNA in HCC progression is difficult. In this study, we selected highly expressed microRNAs with different read counts as candidate microRNAs and then tested whether the microRNAs were differentially expressed in HCC tumour tissues, and we found that their expression was related to the HCC prognosis. Then, we investigated the effects of microRNAs on the cell growth and mobility of HCC using a real-time cell analyser (RTCA), colony formation assay and subcutaneous xenograft models. We further used deep-sequencing technology and bioinformatic analyses to evaluate the main functions of the microRNAs. We found that miR-103a was one of the most highly expressed microRNAs in HCC tissues and that it was upregulated in HCC tissue compared with the controls. In addition, high miR-103a expression was associated with poor patient prognosis, and its overexpression promoted HCC cell growth and mobility. A functional enrichment analysis showed that miR-103a mainly promoted glucose metabolism and inhibited cell death. We validated this analysis, and the data showed that miR-103a promoted glucose metabolism-likely function and directly inhibited cell death via ATP11A and EIF5. Therefore, our study revealed that miR-103a may act as a key mediator in HCC progression.
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spelling pubmed-82060762021-07-01 MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma Liu, Yuling Zhang, Yuanzhou Xiao, Bowen Tang, Ning Hu, Jingying Liang, Shunshun Pang, Yechun Xu, Huili Ao, Junping Yang, Juan Liang, Xiaofei Wei, Lin Wang, Yunfeng Luo, Xiaoying Cell Death Dis Article Hepatocellular carcinoma (HCC) is a common and high-mortality cancer worldwide. Numerous microRNAs have crucial roles in the progression of different cancers. However, identifying the important microRNAs and the target biological function of the microRNA in HCC progression is difficult. In this study, we selected highly expressed microRNAs with different read counts as candidate microRNAs and then tested whether the microRNAs were differentially expressed in HCC tumour tissues, and we found that their expression was related to the HCC prognosis. Then, we investigated the effects of microRNAs on the cell growth and mobility of HCC using a real-time cell analyser (RTCA), colony formation assay and subcutaneous xenograft models. We further used deep-sequencing technology and bioinformatic analyses to evaluate the main functions of the microRNAs. We found that miR-103a was one of the most highly expressed microRNAs in HCC tissues and that it was upregulated in HCC tissue compared with the controls. In addition, high miR-103a expression was associated with poor patient prognosis, and its overexpression promoted HCC cell growth and mobility. A functional enrichment analysis showed that miR-103a mainly promoted glucose metabolism and inhibited cell death. We validated this analysis, and the data showed that miR-103a promoted glucose metabolism-likely function and directly inhibited cell death via ATP11A and EIF5. Therefore, our study revealed that miR-103a may act as a key mediator in HCC progression. Nature Publishing Group UK 2021-06-15 /pmc/articles/PMC8206076/ /pubmed/34131101 http://dx.doi.org/10.1038/s41419-021-03905-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Liu, Yuling
Zhang, Yuanzhou
Xiao, Bowen
Tang, Ning
Hu, Jingying
Liang, Shunshun
Pang, Yechun
Xu, Huili
Ao, Junping
Yang, Juan
Liang, Xiaofei
Wei, Lin
Wang, Yunfeng
Luo, Xiaoying
MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma
title MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma
title_full MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma
title_fullStr MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma
title_full_unstemmed MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma
title_short MiR-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma
title_sort mir-103a promotes tumour growth and influences glucose metabolism in hepatocellular carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206076/
https://www.ncbi.nlm.nih.gov/pubmed/34131101
http://dx.doi.org/10.1038/s41419-021-03905-3
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