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Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees

Microsporidia comprise a phylum of single cell, intracellular parasites and represent the earliest diverging branch in the fungal kingdom. The microsporidian parasite Nosema ceranae primarily infects honey bee gut epithelial cells, leading to impaired memory, suppressed host immune responses and col...

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Autores principales: Huang, Qiang, Wu, Zhi Hao, Li, Wen Feng, Guo, Rui, Xu, Jin Shan, Dang, Xiao Qun, Ma, Zheng Gang, Chen, Yan Ping, Evans, Jay D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206274/
https://www.ncbi.nlm.nih.gov/pubmed/34149635
http://dx.doi.org/10.3389/fmicb.2021.645353
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author Huang, Qiang
Wu, Zhi Hao
Li, Wen Feng
Guo, Rui
Xu, Jin Shan
Dang, Xiao Qun
Ma, Zheng Gang
Chen, Yan Ping
Evans, Jay D.
author_facet Huang, Qiang
Wu, Zhi Hao
Li, Wen Feng
Guo, Rui
Xu, Jin Shan
Dang, Xiao Qun
Ma, Zheng Gang
Chen, Yan Ping
Evans, Jay D.
author_sort Huang, Qiang
collection PubMed
description Microsporidia comprise a phylum of single cell, intracellular parasites and represent the earliest diverging branch in the fungal kingdom. The microsporidian parasite Nosema ceranae primarily infects honey bee gut epithelial cells, leading to impaired memory, suppressed host immune responses and colony collapse under certain circumstances. As the genome of N. ceranae is challenging to assembly due to very high genetic diversity and repetitive region, the genome was re-sequenced using long reads. We present a robust 8.8 Mbp genome assembly of 2,280 protein coding genes, including a high number of genes involved in transporting nutrients and energy, as well as drug resistance when compared with sister species Nosema apis. We also describe the loss of the critical protein Dicer in approximately half of the microsporidian species, giving new insights into the availability of RNA interference pathway in this group. Our results provided new insights into the pathogenesis of N. ceranae and a blueprint for treatment strategies that target this parasite without harming honey bees. The unique infectious apparatus polar filament and transportation pathway members can help to identify treatments to control this parasite.
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spelling pubmed-82062742021-06-17 Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees Huang, Qiang Wu, Zhi Hao Li, Wen Feng Guo, Rui Xu, Jin Shan Dang, Xiao Qun Ma, Zheng Gang Chen, Yan Ping Evans, Jay D. Front Microbiol Microbiology Microsporidia comprise a phylum of single cell, intracellular parasites and represent the earliest diverging branch in the fungal kingdom. The microsporidian parasite Nosema ceranae primarily infects honey bee gut epithelial cells, leading to impaired memory, suppressed host immune responses and colony collapse under certain circumstances. As the genome of N. ceranae is challenging to assembly due to very high genetic diversity and repetitive region, the genome was re-sequenced using long reads. We present a robust 8.8 Mbp genome assembly of 2,280 protein coding genes, including a high number of genes involved in transporting nutrients and energy, as well as drug resistance when compared with sister species Nosema apis. We also describe the loss of the critical protein Dicer in approximately half of the microsporidian species, giving new insights into the availability of RNA interference pathway in this group. Our results provided new insights into the pathogenesis of N. ceranae and a blueprint for treatment strategies that target this parasite without harming honey bees. The unique infectious apparatus polar filament and transportation pathway members can help to identify treatments to control this parasite. Frontiers Media S.A. 2021-06-02 /pmc/articles/PMC8206274/ /pubmed/34149635 http://dx.doi.org/10.3389/fmicb.2021.645353 Text en Copyright © 2021 Huang, Wu, Li, Guo, Xu, Dang, Ma, Chen and Evans. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Huang, Qiang
Wu, Zhi Hao
Li, Wen Feng
Guo, Rui
Xu, Jin Shan
Dang, Xiao Qun
Ma, Zheng Gang
Chen, Yan Ping
Evans, Jay D.
Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
title Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
title_full Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
title_fullStr Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
title_full_unstemmed Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
title_short Genome and Evolutionary Analysis of Nosema ceranae: A Microsporidian Parasite of Honey Bees
title_sort genome and evolutionary analysis of nosema ceranae: a microsporidian parasite of honey bees
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206274/
https://www.ncbi.nlm.nih.gov/pubmed/34149635
http://dx.doi.org/10.3389/fmicb.2021.645353
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