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Design and in silico validation of polymerase chain reaction primers to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Accurate designing of polymerase chain reaction (PCR) primers targeting conserved segments in viral genomes is desirable for preventing false-negative results and decreasing the need for standardization across different PCR protocols. In this work, we designed and described a set of primers and prob...

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Detalles Bibliográficos
Autores principales: Davi, Maria Júlia P., Jeronimo, Selma M. B., Lima, João P. M. S., Lanza, Daniel C. F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206341/
https://www.ncbi.nlm.nih.gov/pubmed/34131209
http://dx.doi.org/10.1038/s41598-021-91817-9