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Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds

Widespread use of antimicrobial drugs has led to high levels of drug-resistance in pathogen populations and a need for novel sources of anti-bacterial and anti-parasitic compounds. Macroalgae (seaweed) are potentially a rich source of bioactive compounds, and several species have traditionally been...

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Autores principales: Bonde, Charlotte Smith, Bornancin, Louis, Lu, Yi, Simonsen, Henrik Toft, Martínez-Valladares, María, Peña-Espinoza, Miguel, Mejer, Helena, Williams, Andrew R., Thamsborg, Stig Milan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206555/
https://www.ncbi.nlm.nih.gov/pubmed/34149425
http://dx.doi.org/10.3389/fphar.2021.674520
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author Bonde, Charlotte Smith
Bornancin, Louis
Lu, Yi
Simonsen, Henrik Toft
Martínez-Valladares, María
Peña-Espinoza, Miguel
Mejer, Helena
Williams, Andrew R.
Thamsborg, Stig Milan
author_facet Bonde, Charlotte Smith
Bornancin, Louis
Lu, Yi
Simonsen, Henrik Toft
Martínez-Valladares, María
Peña-Espinoza, Miguel
Mejer, Helena
Williams, Andrew R.
Thamsborg, Stig Milan
author_sort Bonde, Charlotte Smith
collection PubMed
description Widespread use of antimicrobial drugs has led to high levels of drug-resistance in pathogen populations and a need for novel sources of anti-bacterial and anti-parasitic compounds. Macroalgae (seaweed) are potentially a rich source of bioactive compounds, and several species have traditionally been used as vermifuges. Here, we investigated the anti-parasitic properties of four common cold-water Nordic seaweeds; Palmaria palmata (Rhodophyta), Laminaria digitata, Saccharina latissima and Ascophyllum nodosum (Ochrophyta, Phaeophyceae). Screening of organic extracts against helminths of swine (Ascaris suum) and sheep (Teladorsagia circumcincta) revealed that S. latissima and L. digitata had particularly high biological activity. A combination of molecular networking and bio-guided fractionation led to the isolation of six compounds from extracts of these two species identified in both fermented and non-fermented samples. The six isolated compounds were tentatively identified by using MS-FINDER as five fatty acids and one monoglyceride: Stearidonic acid (1), Eicosapentaenoic acid (2), Alpha-Linolenic acid (3), Docosahexaenoic acid (4), Arachidonic acid (5), and Monoacylglycerol (MG 20:5) (6). Individual compounds showed only modest activity against A. suum, but a clear synergistic effect was apparent when selected compounds were tested in combination. Collectively, our data reveal that fatty acids may have a previously unappreciated role as natural anti-parasitic compounds, which suggests that seaweed products may represent a viable option for control of intestinal helminth infections.
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spelling pubmed-82065552021-06-17 Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds Bonde, Charlotte Smith Bornancin, Louis Lu, Yi Simonsen, Henrik Toft Martínez-Valladares, María Peña-Espinoza, Miguel Mejer, Helena Williams, Andrew R. Thamsborg, Stig Milan Front Pharmacol Pharmacology Widespread use of antimicrobial drugs has led to high levels of drug-resistance in pathogen populations and a need for novel sources of anti-bacterial and anti-parasitic compounds. Macroalgae (seaweed) are potentially a rich source of bioactive compounds, and several species have traditionally been used as vermifuges. Here, we investigated the anti-parasitic properties of four common cold-water Nordic seaweeds; Palmaria palmata (Rhodophyta), Laminaria digitata, Saccharina latissima and Ascophyllum nodosum (Ochrophyta, Phaeophyceae). Screening of organic extracts against helminths of swine (Ascaris suum) and sheep (Teladorsagia circumcincta) revealed that S. latissima and L. digitata had particularly high biological activity. A combination of molecular networking and bio-guided fractionation led to the isolation of six compounds from extracts of these two species identified in both fermented and non-fermented samples. The six isolated compounds were tentatively identified by using MS-FINDER as five fatty acids and one monoglyceride: Stearidonic acid (1), Eicosapentaenoic acid (2), Alpha-Linolenic acid (3), Docosahexaenoic acid (4), Arachidonic acid (5), and Monoacylglycerol (MG 20:5) (6). Individual compounds showed only modest activity against A. suum, but a clear synergistic effect was apparent when selected compounds were tested in combination. Collectively, our data reveal that fatty acids may have a previously unappreciated role as natural anti-parasitic compounds, which suggests that seaweed products may represent a viable option for control of intestinal helminth infections. Frontiers Media S.A. 2021-06-02 /pmc/articles/PMC8206555/ /pubmed/34149425 http://dx.doi.org/10.3389/fphar.2021.674520 Text en Copyright © 2021 Bonde, Bornancin, Lu, Simonsen, Martínez-Valladares, Peña-Espinoza, Mejer, Williams and Thamsborg. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Bonde, Charlotte Smith
Bornancin, Louis
Lu, Yi
Simonsen, Henrik Toft
Martínez-Valladares, María
Peña-Espinoza, Miguel
Mejer, Helena
Williams, Andrew R.
Thamsborg, Stig Milan
Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds
title Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds
title_full Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds
title_fullStr Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds
title_full_unstemmed Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds
title_short Bio-Guided Fractionation and Molecular Networking Reveal Fatty Acids to Be Principal Anti-Parasitic Compounds in Nordic Seaweeds
title_sort bio-guided fractionation and molecular networking reveal fatty acids to be principal anti-parasitic compounds in nordic seaweeds
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206555/
https://www.ncbi.nlm.nih.gov/pubmed/34149425
http://dx.doi.org/10.3389/fphar.2021.674520
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