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The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium

Cellular responses to implanted biomaterials are key to understanding osseointegration. The aim of this investigation was to determine the in vitro priming and activation of the respiratory burst activity of monocytes in response to surface-modified titanium. Human peripheral blood monocytes of heal...

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Autores principales: De Poi, Robert P., Kowolik, Michael, Oshida, Yoshiki, El Kholy, Karim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206560/
https://www.ncbi.nlm.nih.gov/pubmed/34149683
http://dx.doi.org/10.3389/fimmu.2021.618002
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author De Poi, Robert P.
Kowolik, Michael
Oshida, Yoshiki
El Kholy, Karim
author_facet De Poi, Robert P.
Kowolik, Michael
Oshida, Yoshiki
El Kholy, Karim
author_sort De Poi, Robert P.
collection PubMed
description Cellular responses to implanted biomaterials are key to understanding osseointegration. The aim of this investigation was to determine the in vitro priming and activation of the respiratory burst activity of monocytes in response to surface-modified titanium. Human peripheral blood monocytes of healthy blood donors were separated, then incubated with surface-modified grade 2 commercially pure titanium (CPT) disks with a range of known surface energies and surface roughness for 30- or 60-min. Secondary stimulation by phorbol 12-myrisate 13-acetate (PMA) following the priming phase, and luminol-enhanced-chemiluminescence (LCL) was used to monitor oxygen-dependent activity. Comparison among groups was made by incubation time using one-way ANOVA. One sample from each group for each phase of the experiment was viewed under scanning electron microscopy (SEM) and qualitative comparisons made. The results indicate that titanium is capable of priming peripheral blood monocytes following 60-min incubation. In contrast, 30 min incubation time lead to reduced LCL on secondary stimulation as compared to cells alone. At both time intervals, the disk with the lowest surface energy produced significantly less LCL compared to other samples. SEM examination revealed differences in surface morphology at different time points but not between differently surface-modified disks. These results are consistent with the hypothesis that the titanium surface characteristics influenced the monocyte activity, which may be important in regulating the healing response to these materials.
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spelling pubmed-82065602021-06-17 The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium De Poi, Robert P. Kowolik, Michael Oshida, Yoshiki El Kholy, Karim Front Immunol Immunology Cellular responses to implanted biomaterials are key to understanding osseointegration. The aim of this investigation was to determine the in vitro priming and activation of the respiratory burst activity of monocytes in response to surface-modified titanium. Human peripheral blood monocytes of healthy blood donors were separated, then incubated with surface-modified grade 2 commercially pure titanium (CPT) disks with a range of known surface energies and surface roughness for 30- or 60-min. Secondary stimulation by phorbol 12-myrisate 13-acetate (PMA) following the priming phase, and luminol-enhanced-chemiluminescence (LCL) was used to monitor oxygen-dependent activity. Comparison among groups was made by incubation time using one-way ANOVA. One sample from each group for each phase of the experiment was viewed under scanning electron microscopy (SEM) and qualitative comparisons made. The results indicate that titanium is capable of priming peripheral blood monocytes following 60-min incubation. In contrast, 30 min incubation time lead to reduced LCL on secondary stimulation as compared to cells alone. At both time intervals, the disk with the lowest surface energy produced significantly less LCL compared to other samples. SEM examination revealed differences in surface morphology at different time points but not between differently surface-modified disks. These results are consistent with the hypothesis that the titanium surface characteristics influenced the monocyte activity, which may be important in regulating the healing response to these materials. Frontiers Media S.A. 2021-06-02 /pmc/articles/PMC8206560/ /pubmed/34149683 http://dx.doi.org/10.3389/fimmu.2021.618002 Text en Copyright © 2021 De Poi, Kowolik, Oshida and El Kholy https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
De Poi, Robert P.
Kowolik, Michael
Oshida, Yoshiki
El Kholy, Karim
The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium
title The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium
title_full The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium
title_fullStr The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium
title_full_unstemmed The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium
title_short The Oxidative Response of Human Monocytes to Surface Modified Commercially Pure Titanium
title_sort oxidative response of human monocytes to surface modified commercially pure titanium
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206560/
https://www.ncbi.nlm.nih.gov/pubmed/34149683
http://dx.doi.org/10.3389/fimmu.2021.618002
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