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The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy
The first vibrational sum-frequency generation (VSFG) spectra of chondroitin sulfate (CS) interacting with dipalmitoyl phosphatidylcholine (DPPC) at air–liquid interface are reported here, collected at a laser repetition rate of 100 kHz. By studying the VSFG spectra in the regions of 1050–1450 cm(−1...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207512/ https://www.ncbi.nlm.nih.gov/pubmed/34105546 http://dx.doi.org/10.1039/d1cp01975a |
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author | Szekeres, Gergo Peter Krekic, Szilvia Miller, Rebecca L. Mero, Mark Pagel, Kevin Heiner, Zsuzsanna |
author_facet | Szekeres, Gergo Peter Krekic, Szilvia Miller, Rebecca L. Mero, Mark Pagel, Kevin Heiner, Zsuzsanna |
author_sort | Szekeres, Gergo Peter |
collection | PubMed |
description | The first vibrational sum-frequency generation (VSFG) spectra of chondroitin sulfate (CS) interacting with dipalmitoyl phosphatidylcholine (DPPC) at air–liquid interface are reported here, collected at a laser repetition rate of 100 kHz. By studying the VSFG spectra in the regions of 1050–1450 cm(−1), 2750–3180 cm(−1), and 3200–3825 cm(−1), it was concluded that in the presence of Ca(2+) ions, the head groups together with the head-group-bound water molecules in the DPPC monolayer are strongly influenced by the interaction with CS, while the organization of the phospholipid tails remains mostly unchanged. The interactions were observed at a CS concentration below 200 nM, which exemplifies the potential of VSFG in studying biomolecular interactions at low physiological concentrations. The VSFG spectra recorded in the O–H stretching region at chiral polarization combination imply that CS molecules are organized into ordered macromolecular superstructures with a chiral secondary structure. |
format | Online Article Text |
id | pubmed-8207512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-82075122021-06-29 The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy Szekeres, Gergo Peter Krekic, Szilvia Miller, Rebecca L. Mero, Mark Pagel, Kevin Heiner, Zsuzsanna Phys Chem Chem Phys Chemistry The first vibrational sum-frequency generation (VSFG) spectra of chondroitin sulfate (CS) interacting with dipalmitoyl phosphatidylcholine (DPPC) at air–liquid interface are reported here, collected at a laser repetition rate of 100 kHz. By studying the VSFG spectra in the regions of 1050–1450 cm(−1), 2750–3180 cm(−1), and 3200–3825 cm(−1), it was concluded that in the presence of Ca(2+) ions, the head groups together with the head-group-bound water molecules in the DPPC monolayer are strongly influenced by the interaction with CS, while the organization of the phospholipid tails remains mostly unchanged. The interactions were observed at a CS concentration below 200 nM, which exemplifies the potential of VSFG in studying biomolecular interactions at low physiological concentrations. The VSFG spectra recorded in the O–H stretching region at chiral polarization combination imply that CS molecules are organized into ordered macromolecular superstructures with a chiral secondary structure. The Royal Society of Chemistry 2021-06-07 /pmc/articles/PMC8207512/ /pubmed/34105546 http://dx.doi.org/10.1039/d1cp01975a Text en This journal is © the Owner Societies https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Szekeres, Gergo Peter Krekic, Szilvia Miller, Rebecca L. Mero, Mark Pagel, Kevin Heiner, Zsuzsanna The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy |
title | The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy |
title_full | The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy |
title_fullStr | The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy |
title_full_unstemmed | The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy |
title_short | The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy |
title_sort | interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8207512/ https://www.ncbi.nlm.nih.gov/pubmed/34105546 http://dx.doi.org/10.1039/d1cp01975a |
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