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The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2

GTP-binding protein (G-protein) and regulator of G-protein signaling (RGS) mediated signal transduction are critical in the growth and virulence of the rice blast pathogen Magnaporthe oryzae. We have previously reported that there are eight RGS and RGS-like proteins named MoRgs1 to MoRgs8 playing di...

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Autores principales: Yu, Rui, Shen, Xuetong, Liu, Muxing, Liu, Xinyu, Yin, Ziyi, Li, Xiao, Feng, Wanzhen, Hu, Jiexiong, Zhang, Haifeng, Zheng, Xiaobo, Wang, Ping, Zhang, Zhengguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208561/
https://www.ncbi.nlm.nih.gov/pubmed/34133468
http://dx.doi.org/10.1371/journal.ppat.1009657
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author Yu, Rui
Shen, Xuetong
Liu, Muxing
Liu, Xinyu
Yin, Ziyi
Li, Xiao
Feng, Wanzhen
Hu, Jiexiong
Zhang, Haifeng
Zheng, Xiaobo
Wang, Ping
Zhang, Zhengguang
author_facet Yu, Rui
Shen, Xuetong
Liu, Muxing
Liu, Xinyu
Yin, Ziyi
Li, Xiao
Feng, Wanzhen
Hu, Jiexiong
Zhang, Haifeng
Zheng, Xiaobo
Wang, Ping
Zhang, Zhengguang
author_sort Yu, Rui
collection PubMed
description GTP-binding protein (G-protein) and regulator of G-protein signaling (RGS) mediated signal transduction are critical in the growth and virulence of the rice blast pathogen Magnaporthe oryzae. We have previously reported that there are eight RGS and RGS-like proteins named MoRgs1 to MoRgs8 playing distinct and shared regulatory functions in M. oryzae and that MoRgs1 has a more prominent role compared to others in the fungus. To further explore the unique regulatory mechanism of MoRgs1, we screened a M. oryzae cDNA library for genes encoding MoRgs1-interacting proteins and identified MoCkb2, one of the two regulatory subunits of the casein kinase (CK) 2 MoCk2. We found that MoCkb2 and the sole catalytic subunit MoCka1 are required for the phosphorylation of MoRgs1 at the plasma membrane (PM) and late endosome (LE). We further found that an endoplasmic reticulum (ER) membrane protein complex (EMC) subunit, MoEmc2, modulates the phosphorylation of MoRgs1 by MoCk2. Interestingly, this phosphorylation is also essential for the GTPase-activating protein (GAP) function of MoRgs1. The balance among MoRgs1, MoCk2, and MoEmc2 ensures normal operation of the G-protein MoMagA-cAMP signaling required for appressorium formation and pathogenicity of the fungus. This has been the first report that an EMC subunit is directly linked to G-protein signaling through modulation of an RGS-casein kinase interaction.
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spelling pubmed-82085612021-06-29 The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2 Yu, Rui Shen, Xuetong Liu, Muxing Liu, Xinyu Yin, Ziyi Li, Xiao Feng, Wanzhen Hu, Jiexiong Zhang, Haifeng Zheng, Xiaobo Wang, Ping Zhang, Zhengguang PLoS Pathog Research Article GTP-binding protein (G-protein) and regulator of G-protein signaling (RGS) mediated signal transduction are critical in the growth and virulence of the rice blast pathogen Magnaporthe oryzae. We have previously reported that there are eight RGS and RGS-like proteins named MoRgs1 to MoRgs8 playing distinct and shared regulatory functions in M. oryzae and that MoRgs1 has a more prominent role compared to others in the fungus. To further explore the unique regulatory mechanism of MoRgs1, we screened a M. oryzae cDNA library for genes encoding MoRgs1-interacting proteins and identified MoCkb2, one of the two regulatory subunits of the casein kinase (CK) 2 MoCk2. We found that MoCkb2 and the sole catalytic subunit MoCka1 are required for the phosphorylation of MoRgs1 at the plasma membrane (PM) and late endosome (LE). We further found that an endoplasmic reticulum (ER) membrane protein complex (EMC) subunit, MoEmc2, modulates the phosphorylation of MoRgs1 by MoCk2. Interestingly, this phosphorylation is also essential for the GTPase-activating protein (GAP) function of MoRgs1. The balance among MoRgs1, MoCk2, and MoEmc2 ensures normal operation of the G-protein MoMagA-cAMP signaling required for appressorium formation and pathogenicity of the fungus. This has been the first report that an EMC subunit is directly linked to G-protein signaling through modulation of an RGS-casein kinase interaction. Public Library of Science 2021-06-16 /pmc/articles/PMC8208561/ /pubmed/34133468 http://dx.doi.org/10.1371/journal.ppat.1009657 Text en © 2021 Yu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yu, Rui
Shen, Xuetong
Liu, Muxing
Liu, Xinyu
Yin, Ziyi
Li, Xiao
Feng, Wanzhen
Hu, Jiexiong
Zhang, Haifeng
Zheng, Xiaobo
Wang, Ping
Zhang, Zhengguang
The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2
title The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2
title_full The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2
title_fullStr The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2
title_full_unstemmed The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2
title_short The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2
title_sort rice blast fungus morgs1 functioning in camp signaling and pathogenicity is regulated by casein kinase mock2 phosphorylation and modulated by membrane protein moemc2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208561/
https://www.ncbi.nlm.nih.gov/pubmed/34133468
http://dx.doi.org/10.1371/journal.ppat.1009657
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