Cargando…
Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage
CONTEXT: The early events regulating the remodeling program following skeletal muscle damage are poorly understood. OBJECTIVE: The objective of this study was to determine the association between myofibrillar protein synthesis (myoPS) and nuclear factor-kappa B (NF-κB) signaling by nutritionally acc...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208676/ https://www.ncbi.nlm.nih.gov/pubmed/33710344 http://dx.doi.org/10.1210/clinem/dgab106 |
_version_ | 1783708968954101760 |
---|---|
author | Jameson, Tom S O Pavis, George F Dirks, Marlou L Lee, Benjamin P Abdelrahman, Doaa R Murton, Andrew J Porter, Craig Alamdari, Nima Mikus, Catherine R Wall, Benjamin T Stephens, Francis B |
author_facet | Jameson, Tom S O Pavis, George F Dirks, Marlou L Lee, Benjamin P Abdelrahman, Doaa R Murton, Andrew J Porter, Craig Alamdari, Nima Mikus, Catherine R Wall, Benjamin T Stephens, Francis B |
author_sort | Jameson, Tom S O |
collection | PubMed |
description | CONTEXT: The early events regulating the remodeling program following skeletal muscle damage are poorly understood. OBJECTIVE: The objective of this study was to determine the association between myofibrillar protein synthesis (myoPS) and nuclear factor-kappa B (NF-κB) signaling by nutritionally accelerating the recovery of muscle function following damage. DESIGN, SETTING, PARTICIPANTS, AND INTERVENTIONS: Healthy males and females consumed daily postexercise and prebed protein-polyphenol (PP; n = 9; 4 females) or isocaloric maltodextrin placebo (PLA; n = 9; 3 females) drinks (parallel design) 6 days before and 3 days after 300 unilateral eccentric contractions of the quadriceps during complete dietary control. MAIN OUTCOME MEASURES: Muscle function was assessed daily, and skeletal muscle biopsies were taken after 24, 27, and 36 hours for measurements of myoPS rates using deuterated water, and gene ontology and NF-κB signaling analysis using a quantitative reverse transcription PCR (RT-qPCR) gene array. RESULTS: Eccentric contractions impaired muscle function for 48 hours in PLA intervention, but just for 24 hours in PP intervention (P = 0.047). Eccentric quadricep contractions increased myoPS compared with the control leg during postexercise (24–27 hours; 0.14 ± 0.01 vs 0.11 ± 0.01%·h(-1), respectively; P = 0.075) and overnight periods (27–36 hours; 0.10 ± 0.01 vs 0.07 ± 0.01%·h(-1), respectively; P = 0.020), but was not further increased by PP drinks (P > 0.05). Protein-polyphenol drinks decreased postexercise and overnight muscle IL1R1 (PLA = 2.8 ± 0.4, PP = 1.1 ± 0.4 and PLA = 1.9 ± 0.4, PP = 0.3 ± 0.4 log(2) fold-change, respectively) and IL1RL1 (PLA = 4.9 ± 0.7, PP = 1.6 ± 0.8 and PLA = 3.7 ± 0.6, PP = 0.7 ± 0.7 log(2) fold-change, respectively) messenger RNA expression (P < 0.05) and downstream NF-κB signaling compared with PLA. CONCLUSION: Protein-polyphenol drink ingestion likely accelerates recovery of muscle function by attenuating inflammatory NF-κB transcriptional signaling, possibly to reduce aberrant tissue degradation rather than increase myoPS rates. |
format | Online Article Text |
id | pubmed-8208676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-82086762021-06-21 Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage Jameson, Tom S O Pavis, George F Dirks, Marlou L Lee, Benjamin P Abdelrahman, Doaa R Murton, Andrew J Porter, Craig Alamdari, Nima Mikus, Catherine R Wall, Benjamin T Stephens, Francis B J Clin Endocrinol Metab Clinical Research Articles CONTEXT: The early events regulating the remodeling program following skeletal muscle damage are poorly understood. OBJECTIVE: The objective of this study was to determine the association between myofibrillar protein synthesis (myoPS) and nuclear factor-kappa B (NF-κB) signaling by nutritionally accelerating the recovery of muscle function following damage. DESIGN, SETTING, PARTICIPANTS, AND INTERVENTIONS: Healthy males and females consumed daily postexercise and prebed protein-polyphenol (PP; n = 9; 4 females) or isocaloric maltodextrin placebo (PLA; n = 9; 3 females) drinks (parallel design) 6 days before and 3 days after 300 unilateral eccentric contractions of the quadriceps during complete dietary control. MAIN OUTCOME MEASURES: Muscle function was assessed daily, and skeletal muscle biopsies were taken after 24, 27, and 36 hours for measurements of myoPS rates using deuterated water, and gene ontology and NF-κB signaling analysis using a quantitative reverse transcription PCR (RT-qPCR) gene array. RESULTS: Eccentric contractions impaired muscle function for 48 hours in PLA intervention, but just for 24 hours in PP intervention (P = 0.047). Eccentric quadricep contractions increased myoPS compared with the control leg during postexercise (24–27 hours; 0.14 ± 0.01 vs 0.11 ± 0.01%·h(-1), respectively; P = 0.075) and overnight periods (27–36 hours; 0.10 ± 0.01 vs 0.07 ± 0.01%·h(-1), respectively; P = 0.020), but was not further increased by PP drinks (P > 0.05). Protein-polyphenol drinks decreased postexercise and overnight muscle IL1R1 (PLA = 2.8 ± 0.4, PP = 1.1 ± 0.4 and PLA = 1.9 ± 0.4, PP = 0.3 ± 0.4 log(2) fold-change, respectively) and IL1RL1 (PLA = 4.9 ± 0.7, PP = 1.6 ± 0.8 and PLA = 3.7 ± 0.6, PP = 0.7 ± 0.7 log(2) fold-change, respectively) messenger RNA expression (P < 0.05) and downstream NF-κB signaling compared with PLA. CONCLUSION: Protein-polyphenol drink ingestion likely accelerates recovery of muscle function by attenuating inflammatory NF-κB transcriptional signaling, possibly to reduce aberrant tissue degradation rather than increase myoPS rates. Oxford University Press 2021-02-26 /pmc/articles/PMC8208676/ /pubmed/33710344 http://dx.doi.org/10.1210/clinem/dgab106 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Research Articles Jameson, Tom S O Pavis, George F Dirks, Marlou L Lee, Benjamin P Abdelrahman, Doaa R Murton, Andrew J Porter, Craig Alamdari, Nima Mikus, Catherine R Wall, Benjamin T Stephens, Francis B Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage |
title | Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage |
title_full | Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage |
title_fullStr | Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage |
title_full_unstemmed | Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage |
title_short | Reducing NF-κB Signaling Nutritionally is Associated with Expedited Recovery of Skeletal Muscle Function After Damage |
title_sort | reducing nf-κb signaling nutritionally is associated with expedited recovery of skeletal muscle function after damage |
topic | Clinical Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208676/ https://www.ncbi.nlm.nih.gov/pubmed/33710344 http://dx.doi.org/10.1210/clinem/dgab106 |
work_keys_str_mv | AT jamesontomso reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT pavisgeorgef reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT dirksmarloul reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT leebenjaminp reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT abdelrahmandoaar reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT murtonandrewj reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT portercraig reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT alamdarinima reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT mikuscatheriner reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT wallbenjamint reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage AT stephensfrancisb reducingnfkbsignalingnutritionallyisassociatedwithexpeditedrecoveryofskeletalmusclefunctionafterdamage |