Cargando…

Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants

Congenital toxoplasmosis is represented by the transplacental passage of Toxoplasma gondii from the mother to the fetus. Our studies demonstrated that T. gondii developed mechanisms to evade of the host immune response, such as cyclooxygenase (COX)-2 and prostaglandin E(2) (PGE(2)) induction, and th...

Descripción completa

Detalles Bibliográficos
Autores principales: de Souza, Guilherme, Silva, Rafaela José, Milián, Iliana Claudia Balga, Rosini, Alessandra Monteiro, de Araújo, Thádia Evelyn, Teixeira, Samuel Cota, Oliveira, Mário Cézar, Franco, Priscila Silva, da Silva, Claudio Vieira, Mineo, José Roberto, Silva, Neide Maria, Ferro, Eloisa Amália Vieira, Barbosa, Bellisa Freitas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209052/
https://www.ncbi.nlm.nih.gov/pubmed/34135407
http://dx.doi.org/10.1038/s41598-021-92120-3
_version_ 1783709048072306688
author de Souza, Guilherme
Silva, Rafaela José
Milián, Iliana Claudia Balga
Rosini, Alessandra Monteiro
de Araújo, Thádia Evelyn
Teixeira, Samuel Cota
Oliveira, Mário Cézar
Franco, Priscila Silva
da Silva, Claudio Vieira
Mineo, José Roberto
Silva, Neide Maria
Ferro, Eloisa Amália Vieira
Barbosa, Bellisa Freitas
author_facet de Souza, Guilherme
Silva, Rafaela José
Milián, Iliana Claudia Balga
Rosini, Alessandra Monteiro
de Araújo, Thádia Evelyn
Teixeira, Samuel Cota
Oliveira, Mário Cézar
Franco, Priscila Silva
da Silva, Claudio Vieira
Mineo, José Roberto
Silva, Neide Maria
Ferro, Eloisa Amália Vieira
Barbosa, Bellisa Freitas
author_sort de Souza, Guilherme
collection PubMed
description Congenital toxoplasmosis is represented by the transplacental passage of Toxoplasma gondii from the mother to the fetus. Our studies demonstrated that T. gondii developed mechanisms to evade of the host immune response, such as cyclooxygenase (COX)-2 and prostaglandin E(2) (PGE(2)) induction, and these mediators can be produced/stored in lipid droplets (LDs). The aim of this study was to evaluate the role of COX-2 and LDs during T. gondii infection in human trophoblast cells and villous explants. Our data demonstrated that COX-2 inhibitors decreased T. gondii replication in trophoblast cells and villous. In BeWo cells, the COX-2 inhibitors induced an increase of pro-inflammatory cytokines (IL-6 and MIF), and a decrease in anti-inflammatory cytokines (IL-4 and IL-10). In HTR-8/SVneo cells, the COX-2 inhibitors induced an increase of IL-6 and nitrite and decreased IL-4 and TGF-β1. In villous explants, the COX-2 inhibitors increased MIF and decreased TNF-α and IL-10. Furthermore, T. gondii induced an increase in LDs in BeWo and HTR-8/SVneo, but COX-2 inhibitors reduced LDs in both cells type. We highlighted that COX-2 is a key factor to T. gondii proliferation in human trophoblast cells, since its inhibition induced a pro-inflammatory response capable of controlling parasitism and leading to a decrease in the availability of LDs, which are essentials for parasite growth.
format Online
Article
Text
id pubmed-8209052
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-82090522021-06-17 Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants de Souza, Guilherme Silva, Rafaela José Milián, Iliana Claudia Balga Rosini, Alessandra Monteiro de Araújo, Thádia Evelyn Teixeira, Samuel Cota Oliveira, Mário Cézar Franco, Priscila Silva da Silva, Claudio Vieira Mineo, José Roberto Silva, Neide Maria Ferro, Eloisa Amália Vieira Barbosa, Bellisa Freitas Sci Rep Article Congenital toxoplasmosis is represented by the transplacental passage of Toxoplasma gondii from the mother to the fetus. Our studies demonstrated that T. gondii developed mechanisms to evade of the host immune response, such as cyclooxygenase (COX)-2 and prostaglandin E(2) (PGE(2)) induction, and these mediators can be produced/stored in lipid droplets (LDs). The aim of this study was to evaluate the role of COX-2 and LDs during T. gondii infection in human trophoblast cells and villous explants. Our data demonstrated that COX-2 inhibitors decreased T. gondii replication in trophoblast cells and villous. In BeWo cells, the COX-2 inhibitors induced an increase of pro-inflammatory cytokines (IL-6 and MIF), and a decrease in anti-inflammatory cytokines (IL-4 and IL-10). In HTR-8/SVneo cells, the COX-2 inhibitors induced an increase of IL-6 and nitrite and decreased IL-4 and TGF-β1. In villous explants, the COX-2 inhibitors increased MIF and decreased TNF-α and IL-10. Furthermore, T. gondii induced an increase in LDs in BeWo and HTR-8/SVneo, but COX-2 inhibitors reduced LDs in both cells type. We highlighted that COX-2 is a key factor to T. gondii proliferation in human trophoblast cells, since its inhibition induced a pro-inflammatory response capable of controlling parasitism and leading to a decrease in the availability of LDs, which are essentials for parasite growth. Nature Publishing Group UK 2021-06-16 /pmc/articles/PMC8209052/ /pubmed/34135407 http://dx.doi.org/10.1038/s41598-021-92120-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
de Souza, Guilherme
Silva, Rafaela José
Milián, Iliana Claudia Balga
Rosini, Alessandra Monteiro
de Araújo, Thádia Evelyn
Teixeira, Samuel Cota
Oliveira, Mário Cézar
Franco, Priscila Silva
da Silva, Claudio Vieira
Mineo, José Roberto
Silva, Neide Maria
Ferro, Eloisa Amália Vieira
Barbosa, Bellisa Freitas
Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
title Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
title_full Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
title_fullStr Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
title_full_unstemmed Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
title_short Cyclooxygenase (COX)-2 modulates Toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
title_sort cyclooxygenase (cox)-2 modulates toxoplasma gondii infection, immune response and lipid droplets formation in human trophoblast cells and villous explants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209052/
https://www.ncbi.nlm.nih.gov/pubmed/34135407
http://dx.doi.org/10.1038/s41598-021-92120-3
work_keys_str_mv AT desouzaguilherme cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT silvarafaelajose cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT milianilianaclaudiabalga cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT rosinialessandramonteiro cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT dearaujothadiaevelyn cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT teixeirasamuelcota cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT oliveiramariocezar cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT francopriscilasilva cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT dasilvaclaudiovieira cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT mineojoseroberto cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT silvaneidemaria cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT ferroeloisaamaliavieira cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants
AT barbosabellisafreitas cyclooxygenasecox2modulatestoxoplasmagondiiinfectionimmuneresponseandlipiddropletsformationinhumantrophoblastcellsandvillousexplants