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Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens

Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts. The rapid diagnosis, in rural and remote area...

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Autores principales: Jalali, Tahmineh, Salehi-Vaziri, Mostafa, Pouriayevali, Mohammad Hassan, Gargari, Seyed Latif Mousavi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209218/
https://www.ncbi.nlm.nih.gov/pubmed/34135365
http://dx.doi.org/10.1038/s41598-021-91826-8
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author Jalali, Tahmineh
Salehi-Vaziri, Mostafa
Pouriayevali, Mohammad Hassan
Gargari, Seyed Latif Mousavi
author_facet Jalali, Tahmineh
Salehi-Vaziri, Mostafa
Pouriayevali, Mohammad Hassan
Gargari, Seyed Latif Mousavi
author_sort Jalali, Tahmineh
collection PubMed
description Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts. The rapid diagnosis, in rural and remote areas where the majority of cases occur, is essential for patient management. Aptamers are considered as a specific and sensitive tool for being used in rapid diagnostic methods. The Nucleoprotein (NP) of the CCHF virus (CCHFV) was selected as the target for the isolation of aptamers based on its abundance and conservative structure, among other viral proteins. A total of 120 aptamers were obtained through 9 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment) from the ssDNA aptamer library, including the random 40-nucleotide ssDNA region between primer binding sites (GCCTGTTGTGAGCCTCCTAAC(N(40))GGGAGACAAGAATAAGCA). The K(D) of aptamers was calculated using the SPR technique. The Apt33 with the highest affinity to NP was selected to design the aptamer-antibody ELASA test. It successfully detected CCHF NP in the concentration of 90 ng/ml in human serum. Evaluation of aptamer-antibody ELASA with clinical samples showed 100% specificity and sensitivity of the test. This simple, specific, and the sensitive assay can be used as a rapid and early diagnosis tool, as well as the use of this aptamer in point of care test near the patient. Our results suggest that the discovered aptamer can be used in various aptamer-based rapid diagnostic tests for the diagnosis of CCHF virus infection.
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spelling pubmed-82092182021-06-17 Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens Jalali, Tahmineh Salehi-Vaziri, Mostafa Pouriayevali, Mohammad Hassan Gargari, Seyed Latif Mousavi Sci Rep Article Crimean-Congo hemorrhagic fever (CCHF) is an acute viral zoonotic disease. The widespread geographic distribution of the disease and the increase in the incidence of the disease from new regions, placed CCHF in a list of public health emergency contexts. The rapid diagnosis, in rural and remote areas where the majority of cases occur, is essential for patient management. Aptamers are considered as a specific and sensitive tool for being used in rapid diagnostic methods. The Nucleoprotein (NP) of the CCHF virus (CCHFV) was selected as the target for the isolation of aptamers based on its abundance and conservative structure, among other viral proteins. A total of 120 aptamers were obtained through 9 rounds of SELEX (Systematic Evolution of Ligands by Exponential Enrichment) from the ssDNA aptamer library, including the random 40-nucleotide ssDNA region between primer binding sites (GCCTGTTGTGAGCCTCCTAAC(N(40))GGGAGACAAGAATAAGCA). The K(D) of aptamers was calculated using the SPR technique. The Apt33 with the highest affinity to NP was selected to design the aptamer-antibody ELASA test. It successfully detected CCHF NP in the concentration of 90 ng/ml in human serum. Evaluation of aptamer-antibody ELASA with clinical samples showed 100% specificity and sensitivity of the test. This simple, specific, and the sensitive assay can be used as a rapid and early diagnosis tool, as well as the use of this aptamer in point of care test near the patient. Our results suggest that the discovered aptamer can be used in various aptamer-based rapid diagnostic tests for the diagnosis of CCHF virus infection. Nature Publishing Group UK 2021-06-16 /pmc/articles/PMC8209218/ /pubmed/34135365 http://dx.doi.org/10.1038/s41598-021-91826-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Jalali, Tahmineh
Salehi-Vaziri, Mostafa
Pouriayevali, Mohammad Hassan
Gargari, Seyed Latif Mousavi
Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
title Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
title_full Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
title_fullStr Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
title_full_unstemmed Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
title_short Aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
title_sort aptamer based diagnosis of crimean-congo hemorrhagic fever from clinical specimens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209218/
https://www.ncbi.nlm.nih.gov/pubmed/34135365
http://dx.doi.org/10.1038/s41598-021-91826-8
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