Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions

We investigated variations in cell growth and ATP Sulfurylase (ATPS) activity when two cyanobacterial strains—Synechocystis sp. PCC6803 and Synechococcus sp. WH7803—were grown in conventional media, and media with low ammonium, low sulfate and a high CO(2)/low O(2) atmosphere. In both organisms, a t...

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Detalles Bibliográficos
Autores principales: Gastoldi, Lucia, Ward, Lewis M., Nakagawa, Mayuko, Giordano, Mario, McGlynn, Shawn E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209453/
https://www.ncbi.nlm.nih.gov/pubmed/34039816
http://dx.doi.org/10.1264/jsme2.ME20145
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author Gastoldi, Lucia
Ward, Lewis M.
Nakagawa, Mayuko
Giordano, Mario
McGlynn, Shawn E.
author_facet Gastoldi, Lucia
Ward, Lewis M.
Nakagawa, Mayuko
Giordano, Mario
McGlynn, Shawn E.
author_sort Gastoldi, Lucia
collection PubMed
description We investigated variations in cell growth and ATP Sulfurylase (ATPS) activity when two cyanobacterial strains—Synechocystis sp. PCC6803 and Synechococcus sp. WH7803—were grown in conventional media, and media with low ammonium, low sulfate and a high CO(2)/low O(2) atmosphere. In both organisms, a transition and adaptation to the reconstructed environmental media resulted in a decrease in ATPS activity. This variation appears to be decoupled from growth rate, suggesting the enzyme is not rate-limiting in S assimilation and raising questions about the role of ATPS redox regulation in cell physiology and throughout Earth history.
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spelling pubmed-82094532021-06-30 Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions Gastoldi, Lucia Ward, Lewis M. Nakagawa, Mayuko Giordano, Mario McGlynn, Shawn E. Microbes Environ Short Communication We investigated variations in cell growth and ATP Sulfurylase (ATPS) activity when two cyanobacterial strains—Synechocystis sp. PCC6803 and Synechococcus sp. WH7803—were grown in conventional media, and media with low ammonium, low sulfate and a high CO(2)/low O(2) atmosphere. In both organisms, a transition and adaptation to the reconstructed environmental media resulted in a decrease in ATPS activity. This variation appears to be decoupled from growth rate, suggesting the enzyme is not rate-limiting in S assimilation and raising questions about the role of ATPS redox regulation in cell physiology and throughout Earth history. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021 2021-05-25 /pmc/articles/PMC8209453/ /pubmed/34039816 http://dx.doi.org/10.1264/jsme2.ME20145 Text en 2021 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Gastoldi, Lucia
Ward, Lewis M.
Nakagawa, Mayuko
Giordano, Mario
McGlynn, Shawn E.
Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions
title Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions
title_full Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions
title_fullStr Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions
title_full_unstemmed Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions
title_short Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions
title_sort changes in atp sulfurylase activity in response to altered cyanobacteria growth conditions
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209453/
https://www.ncbi.nlm.nih.gov/pubmed/34039816
http://dx.doi.org/10.1264/jsme2.ME20145
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