Cargando…

An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep

This study was carried out to determine the presence of polymorphisms in genes affecting litter size. The SNPs in bone morphogenetic protein receptor type 1B (BMPR1B), bone morphogenetic protein 15 (BMP15), and growth differentiation factor 9 (GDF9) genes were detected in 60 uniparous and 60 multipa...

Descripción completa

Detalles Bibliográficos
Autores principales: Çelikeloğlu, Koray, Tekerli, Mustafa, Erdoğan, Metin, Koçak, Serdar, Hacan, Özlem, Bozkurt, Zehra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Copernicus GmbH 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209503/
https://www.ncbi.nlm.nih.gov/pubmed/34159253
http://dx.doi.org/10.5194/aab-64-223-2021
_version_ 1783709142917054464
author Çelikeloğlu, Koray
Tekerli, Mustafa
Erdoğan, Metin
Koçak, Serdar
Hacan, Özlem
Bozkurt, Zehra
author_facet Çelikeloğlu, Koray
Tekerli, Mustafa
Erdoğan, Metin
Koçak, Serdar
Hacan, Özlem
Bozkurt, Zehra
author_sort Çelikeloğlu, Koray
collection PubMed
description This study was carried out to determine the presence of polymorphisms in genes affecting litter size. The SNPs in bone morphogenetic protein receptor type 1B (BMPR1B), bone morphogenetic protein 15 (BMP15), and growth differentiation factor 9 (GDF9) genes were detected in 60 uniparous and 60 multiparous ewes from Ramlıç and Dağlıç breeds. The ewes are maintained in nine public herds at the breeding station of the Afyonkarahisar Sheep and Goats Breeders' Association and lambed in two consecutive breeding seasons. PCR and DNA sequencing analyses were conducted, and 36, 4, and 11 SNPs in Ramlıç and 40, 3, and 11 SNPs in Dağlıç were detected in BMPR1B, BMP15, and GDF9 genes, respectively. A total of 16 SNPs in Ramlıç and 10 SNPs in Dağlıç breeds for three genes were found to be significant ([Formula: see text]). The resulting analyses showed that four SNPs (g.49496G>A, c.1658A>C, c.2037C>T, c.2053C>T) of the BMPR1B gene and one deletion mutation (c.28_30delCTT) in the BMP15 gene of the Ramlıç breed as well as five SNPs (c.1487C>A, c.2492C>T, c.2523G>A, c.2880A>G, and c.2763G>A) of the BMPR1B gene of the Dağlıç breed have significant positive regression coefficients in the desired direction of the rare allele. The observed mutations have potential to be used as genetic markers in the selection of prolific animals for both breeds.
format Online
Article
Text
id pubmed-8209503
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Copernicus GmbH
record_format MEDLINE/PubMed
spelling pubmed-82095032021-06-21 An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep Çelikeloğlu, Koray Tekerli, Mustafa Erdoğan, Metin Koçak, Serdar Hacan, Özlem Bozkurt, Zehra Arch Anim Breed Original Study This study was carried out to determine the presence of polymorphisms in genes affecting litter size. The SNPs in bone morphogenetic protein receptor type 1B (BMPR1B), bone morphogenetic protein 15 (BMP15), and growth differentiation factor 9 (GDF9) genes were detected in 60 uniparous and 60 multiparous ewes from Ramlıç and Dağlıç breeds. The ewes are maintained in nine public herds at the breeding station of the Afyonkarahisar Sheep and Goats Breeders' Association and lambed in two consecutive breeding seasons. PCR and DNA sequencing analyses were conducted, and 36, 4, and 11 SNPs in Ramlıç and 40, 3, and 11 SNPs in Dağlıç were detected in BMPR1B, BMP15, and GDF9 genes, respectively. A total of 16 SNPs in Ramlıç and 10 SNPs in Dağlıç breeds for three genes were found to be significant ([Formula: see text]). The resulting analyses showed that four SNPs (g.49496G>A, c.1658A>C, c.2037C>T, c.2053C>T) of the BMPR1B gene and one deletion mutation (c.28_30delCTT) in the BMP15 gene of the Ramlıç breed as well as five SNPs (c.1487C>A, c.2492C>T, c.2523G>A, c.2880A>G, and c.2763G>A) of the BMPR1B gene of the Dağlıç breed have significant positive regression coefficients in the desired direction of the rare allele. The observed mutations have potential to be used as genetic markers in the selection of prolific animals for both breeds. Copernicus GmbH 2021-06-03 /pmc/articles/PMC8209503/ /pubmed/34159253 http://dx.doi.org/10.5194/aab-64-223-2021 Text en Copyright: © 2021 Koray Çelikeloğlu et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/
spellingShingle Original Study
Çelikeloğlu, Koray
Tekerli, Mustafa
Erdoğan, Metin
Koçak, Serdar
Hacan, Özlem
Bozkurt, Zehra
An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep
title An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep
title_full An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep
title_fullStr An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep
title_full_unstemmed An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep
title_short An investigation of the effects of BMPR1B, BMP15, and GDF9 genes on litter size in Ramlıç and Dağlıç sheep
title_sort investigation of the effects of bmpr1b, bmp15, and gdf9 genes on litter size in ramlıç and dağlıç sheep
topic Original Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209503/
https://www.ncbi.nlm.nih.gov/pubmed/34159253
http://dx.doi.org/10.5194/aab-64-223-2021
work_keys_str_mv AT celikeloglukoray aninvestigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT tekerlimustafa aninvestigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT erdoganmetin aninvestigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT kocakserdar aninvestigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT hacanozlem aninvestigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT bozkurtzehra aninvestigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT celikeloglukoray investigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT tekerlimustafa investigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT erdoganmetin investigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT kocakserdar investigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT hacanozlem investigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep
AT bozkurtzehra investigationoftheeffectsofbmpr1bbmp15andgdf9genesonlittersizeinramlıcanddaglıcsheep