Protocol for the generation of murine bronchiolospheres

Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a detailed...

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Detalles Bibliográficos
Autores principales: Vazquez-Armendariz, Ana Ivonne, Seeger, Werner, Herold, Susanne, El Agha, Elie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209683/
https://www.ncbi.nlm.nih.gov/pubmed/34169288
http://dx.doi.org/10.1016/j.xpro.2021.100594
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author Vazquez-Armendariz, Ana Ivonne
Seeger, Werner
Herold, Susanne
El Agha, Elie
author_facet Vazquez-Armendariz, Ana Ivonne
Seeger, Werner
Herold, Susanne
El Agha, Elie
author_sort Vazquez-Armendariz, Ana Ivonne
collection PubMed
description Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a detailed protocol for culturing airway organoids, or bronchiolospheres, which provide an assessment of the ability of mesenchymal cells to support club-cell growth. For complete details on the use and execution of this protocol, please refer to Moiseenko et al. (2020).
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spelling pubmed-82096832021-06-23 Protocol for the generation of murine bronchiolospheres Vazquez-Armendariz, Ana Ivonne Seeger, Werner Herold, Susanne El Agha, Elie STAR Protoc Protocol Organoid models have been shown to be valuable tools for studying epithelial-mesenchymal crosstalk during biological and pathological settings. Our data identified ACTA2+ PDGFRα+ repair-supportive mesenchymal cells as an important component of the conducting airway niche. Here, we provide a detailed protocol for culturing airway organoids, or bronchiolospheres, which provide an assessment of the ability of mesenchymal cells to support club-cell growth. For complete details on the use and execution of this protocol, please refer to Moiseenko et al. (2020). Elsevier 2021-06-11 /pmc/articles/PMC8209683/ /pubmed/34169288 http://dx.doi.org/10.1016/j.xpro.2021.100594 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Vazquez-Armendariz, Ana Ivonne
Seeger, Werner
Herold, Susanne
El Agha, Elie
Protocol for the generation of murine bronchiolospheres
title Protocol for the generation of murine bronchiolospheres
title_full Protocol for the generation of murine bronchiolospheres
title_fullStr Protocol for the generation of murine bronchiolospheres
title_full_unstemmed Protocol for the generation of murine bronchiolospheres
title_short Protocol for the generation of murine bronchiolospheres
title_sort protocol for the generation of murine bronchiolospheres
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209683/
https://www.ncbi.nlm.nih.gov/pubmed/34169288
http://dx.doi.org/10.1016/j.xpro.2021.100594
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