In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions

Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finall...

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Detalles Bibliográficos
Autores principales: Li, Sheng, Zhang, Guoan, Liu, Yanping, Sun, Qinru, Yang, Lu, Dong, Junli, Zhang, Bowen, Chen, Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209693/
https://www.ncbi.nlm.nih.gov/pubmed/34169294
http://dx.doi.org/10.1016/j.xpro.2021.100601
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author Li, Sheng
Zhang, Guoan
Liu, Yanping
Sun, Qinru
Yang, Lu
Dong, Junli
Zhang, Bowen
Chen, Su
author_facet Li, Sheng
Zhang, Guoan
Liu, Yanping
Sun, Qinru
Yang, Lu
Dong, Junli
Zhang, Bowen
Chen, Su
author_sort Li, Sheng
collection PubMed
description Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).
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spelling pubmed-82096932021-06-23 In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions Li, Sheng Zhang, Guoan Liu, Yanping Sun, Qinru Yang, Lu Dong, Junli Zhang, Bowen Chen, Su STAR Protoc Protocol Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021). Elsevier 2021-06-11 /pmc/articles/PMC8209693/ /pubmed/34169294 http://dx.doi.org/10.1016/j.xpro.2021.100601 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Sheng
Zhang, Guoan
Liu, Yanping
Sun, Qinru
Yang, Lu
Dong, Junli
Zhang, Bowen
Chen, Su
In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions
title In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions
title_full In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions
title_fullStr In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions
title_full_unstemmed In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions
title_short In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions
title_sort in vitro ubiquitination and degradation of bacteria-purified human histone h2b by the nucleoli fractions
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209693/
https://www.ncbi.nlm.nih.gov/pubmed/34169294
http://dx.doi.org/10.1016/j.xpro.2021.100601
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