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In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions
Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finall...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209693/ https://www.ncbi.nlm.nih.gov/pubmed/34169294 http://dx.doi.org/10.1016/j.xpro.2021.100601 |