Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes

During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology i...

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Detalles Bibliográficos
Autores principales: Malvaut, Sarah, Marymonchyk, Alina, Gengatharan, Archana, Saghatelyan, Armen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209737/
https://www.ncbi.nlm.nih.gov/pubmed/34169290
http://dx.doi.org/10.1016/j.xpro.2021.100596
Descripción
Sumario:During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca(2+) activity can be performed continuously for 2–3 days and even up to several months. For complete details on the use and execution of this protocol, please refer to Gengatharan et al. (2021).

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