Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes

During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology i...

Descripción completa

Detalles Bibliográficos
Autores principales: Malvaut, Sarah, Marymonchyk, Alina, Gengatharan, Archana, Saghatelyan, Armen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209737/
https://www.ncbi.nlm.nih.gov/pubmed/34169290
http://dx.doi.org/10.1016/j.xpro.2021.100596
_version_ 1783709188809031680
author Malvaut, Sarah
Marymonchyk, Alina
Gengatharan, Archana
Saghatelyan, Armen
author_facet Malvaut, Sarah
Marymonchyk, Alina
Gengatharan, Archana
Saghatelyan, Armen
author_sort Malvaut, Sarah
collection PubMed
description During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca(2+) activity can be performed continuously for 2–3 days and even up to several months. For complete details on the use and execution of this protocol, please refer to Gengatharan et al. (2021).
format Online
Article
Text
id pubmed-8209737
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-82097372021-06-23 Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes Malvaut, Sarah Marymonchyk, Alina Gengatharan, Archana Saghatelyan, Armen STAR Protoc Protocol During adulthood, the activation of adult neural stem cells (NSCs) has been mostly studied ex vivo in post-mortem tissues or in vivo in anesthetized animals. This protocol presents an approach that allows for the long-term and minimally invasive investigation of adult NSC activation and physiology in freely behaving animals. By combining specific NSC labeling and mini-endoscopic microscopy, live imaging of NSC division and Ca(2+) activity can be performed continuously for 2–3 days and even up to several months. For complete details on the use and execution of this protocol, please refer to Gengatharan et al. (2021). Elsevier 2021-06-12 /pmc/articles/PMC8209737/ /pubmed/34169290 http://dx.doi.org/10.1016/j.xpro.2021.100596 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Malvaut, Sarah
Marymonchyk, Alina
Gengatharan, Archana
Saghatelyan, Armen
Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
title Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
title_full Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
title_fullStr Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
title_full_unstemmed Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
title_short Live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
title_sort live imaging of adult neural stem cells in freely behaving mice using mini-endoscopes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209737/
https://www.ncbi.nlm.nih.gov/pubmed/34169290
http://dx.doi.org/10.1016/j.xpro.2021.100596
work_keys_str_mv AT malvautsarah liveimagingofadultneuralstemcellsinfreelybehavingmiceusingminiendoscopes
AT marymonchykalina liveimagingofadultneuralstemcellsinfreelybehavingmiceusingminiendoscopes
AT gengatharanarchana liveimagingofadultneuralstemcellsinfreelybehavingmiceusingminiendoscopes
AT saghatelyanarmen liveimagingofadultneuralstemcellsinfreelybehavingmiceusingminiendoscopes

Ejemplares similares