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Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection

[Image: see text] Influenza A virus (IAV) poses a significant threat to human health, which calls for the development of efficient detection methods. The present study constructed a fluorescence resonance energy transfer (FRET) system based on novel fluorescent probes and graphene oxide (GO) for det...

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Autores principales: Zhao, Qiuzi, Du, Ping, Wang, Xiaoyong, Huang, Mengqian, Sun, Ling-Dong, Wang, Tao, Wang, Zhiyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210407/
https://www.ncbi.nlm.nih.gov/pubmed/34151102
http://dx.doi.org/10.1021/acsomega.1c01491
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author Zhao, Qiuzi
Du, Ping
Wang, Xiaoyong
Huang, Mengqian
Sun, Ling-Dong
Wang, Tao
Wang, Zhiyun
author_facet Zhao, Qiuzi
Du, Ping
Wang, Xiaoyong
Huang, Mengqian
Sun, Ling-Dong
Wang, Tao
Wang, Zhiyun
author_sort Zhao, Qiuzi
collection PubMed
description [Image: see text] Influenza A virus (IAV) poses a significant threat to human health, which calls for the development of efficient detection methods. The present study constructed a fluorescence resonance energy transfer (FRET) system based on novel fluorescent probes and graphene oxide (GO) for detecting H5N1 IAV hemagglutinin (HA). Here, we synthesized small (sub-20 nm) sandwich-structured upconversion nanoparticles (UCNPs) (SWUCNPs for short) with a high energy transfer efficiency, which allows for controlling the emitter in a thin shell. The π–π stacking interaction between the aptamer and GO shortens the distance between the fluorescent probe and the receptor, thereby realizing fluorescence resonance energy transfer (FRET). When HA is present, the aptamer enables changes in their conformations and move away from GO surface. Fluorescence signals display a linear relationship between HA quantitation in the range of 0.1–15 ng mL(–1) and a limit of detection (LOD) of 60.9 pg mL(–1). The aptasensor was also applicable in human serum samples with a linear range from 0.2 to 12 ng mL(–1) and a limit of detection of 114.7 pg mL(–1). This strategy suggested the promising prospect of the aptasensor in clinical applications because of the excellent sensing performance and sensitivity. This strategy may be promising for vitro diagnostics and provides new insights into the functioning of the SWUCNPs system.
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spelling pubmed-82104072021-06-17 Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection Zhao, Qiuzi Du, Ping Wang, Xiaoyong Huang, Mengqian Sun, Ling-Dong Wang, Tao Wang, Zhiyun ACS Omega [Image: see text] Influenza A virus (IAV) poses a significant threat to human health, which calls for the development of efficient detection methods. The present study constructed a fluorescence resonance energy transfer (FRET) system based on novel fluorescent probes and graphene oxide (GO) for detecting H5N1 IAV hemagglutinin (HA). Here, we synthesized small (sub-20 nm) sandwich-structured upconversion nanoparticles (UCNPs) (SWUCNPs for short) with a high energy transfer efficiency, which allows for controlling the emitter in a thin shell. The π–π stacking interaction between the aptamer and GO shortens the distance between the fluorescent probe and the receptor, thereby realizing fluorescence resonance energy transfer (FRET). When HA is present, the aptamer enables changes in their conformations and move away from GO surface. Fluorescence signals display a linear relationship between HA quantitation in the range of 0.1–15 ng mL(–1) and a limit of detection (LOD) of 60.9 pg mL(–1). The aptasensor was also applicable in human serum samples with a linear range from 0.2 to 12 ng mL(–1) and a limit of detection of 114.7 pg mL(–1). This strategy suggested the promising prospect of the aptasensor in clinical applications because of the excellent sensing performance and sensitivity. This strategy may be promising for vitro diagnostics and provides new insights into the functioning of the SWUCNPs system. American Chemical Society 2021-06-04 /pmc/articles/PMC8210407/ /pubmed/34151102 http://dx.doi.org/10.1021/acsomega.1c01491 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Zhao, Qiuzi
Du, Ping
Wang, Xiaoyong
Huang, Mengqian
Sun, Ling-Dong
Wang, Tao
Wang, Zhiyun
Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
title Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
title_full Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
title_fullStr Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
title_full_unstemmed Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
title_short Upconversion Fluorescence Resonance Energy Transfer Aptasensors for H5N1 Influenza Virus Detection
title_sort upconversion fluorescence resonance energy transfer aptasensors for h5n1 influenza virus detection
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210407/
https://www.ncbi.nlm.nih.gov/pubmed/34151102
http://dx.doi.org/10.1021/acsomega.1c01491
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