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Heterologous production of cyanobacterial compounds

Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in p...

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Autores principales: Dhakal, Dipesh, Chen, Manyun, Luesch, Hendrik, Ding, Yousong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210676/
https://www.ncbi.nlm.nih.gov/pubmed/33928376
http://dx.doi.org/10.1093/jimb/kuab003
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author Dhakal, Dipesh
Chen, Manyun
Luesch, Hendrik
Ding, Yousong
author_facet Dhakal, Dipesh
Chen, Manyun
Luesch, Hendrik
Ding, Yousong
author_sort Dhakal, Dipesh
collection PubMed
description Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in producing new natural products. However, the discovery of these compounds based on cyanobacterial genomes has progressed slowly as the majority of their corresponding biosynthetic gene clusters (BGCs) are silent. In addition, cyanobacterial strains are often slow-growing, difficult for genetic engineering, or cannot be cultivated yet, limiting the use of host genetic engineering approaches for discovery. On the other hand, genetically tractable hosts such as Escherichia coli, Actinobacteria, and yeast have been developed for the heterologous expression of cyanobacterial BGCs. More recently, there have been increased interests in developing model cyanobacterial strains as heterologous production platforms. Herein, we present recent advances in the heterologous production of cyanobacterial compounds in both cyanobacterial and noncyanobacterial hosts. Emerging strategies for BGC assembly, host engineering, and optimization of BGC expression are included for fostering the broader applications of synthetic biology tools in the discovery of new cyanobacterial natural products.
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spelling pubmed-82106762021-06-17 Heterologous production of cyanobacterial compounds Dhakal, Dipesh Chen, Manyun Luesch, Hendrik Ding, Yousong J Ind Microbiol Biotechnol Natural Products Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in producing new natural products. However, the discovery of these compounds based on cyanobacterial genomes has progressed slowly as the majority of their corresponding biosynthetic gene clusters (BGCs) are silent. In addition, cyanobacterial strains are often slow-growing, difficult for genetic engineering, or cannot be cultivated yet, limiting the use of host genetic engineering approaches for discovery. On the other hand, genetically tractable hosts such as Escherichia coli, Actinobacteria, and yeast have been developed for the heterologous expression of cyanobacterial BGCs. More recently, there have been increased interests in developing model cyanobacterial strains as heterologous production platforms. Herein, we present recent advances in the heterologous production of cyanobacterial compounds in both cyanobacterial and noncyanobacterial hosts. Emerging strategies for BGC assembly, host engineering, and optimization of BGC expression are included for fostering the broader applications of synthetic biology tools in the discovery of new cyanobacterial natural products. Oxford University Press 2021-01-25 /pmc/articles/PMC8210676/ /pubmed/33928376 http://dx.doi.org/10.1093/jimb/kuab003 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Natural Products
Dhakal, Dipesh
Chen, Manyun
Luesch, Hendrik
Ding, Yousong
Heterologous production of cyanobacterial compounds
title Heterologous production of cyanobacterial compounds
title_full Heterologous production of cyanobacterial compounds
title_fullStr Heterologous production of cyanobacterial compounds
title_full_unstemmed Heterologous production of cyanobacterial compounds
title_short Heterologous production of cyanobacterial compounds
title_sort heterologous production of cyanobacterial compounds
topic Natural Products
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210676/
https://www.ncbi.nlm.nih.gov/pubmed/33928376
http://dx.doi.org/10.1093/jimb/kuab003
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