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Heterologous production of cyanobacterial compounds
Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210676/ https://www.ncbi.nlm.nih.gov/pubmed/33928376 http://dx.doi.org/10.1093/jimb/kuab003 |
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author | Dhakal, Dipesh Chen, Manyun Luesch, Hendrik Ding, Yousong |
author_facet | Dhakal, Dipesh Chen, Manyun Luesch, Hendrik Ding, Yousong |
author_sort | Dhakal, Dipesh |
collection | PubMed |
description | Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in producing new natural products. However, the discovery of these compounds based on cyanobacterial genomes has progressed slowly as the majority of their corresponding biosynthetic gene clusters (BGCs) are silent. In addition, cyanobacterial strains are often slow-growing, difficult for genetic engineering, or cannot be cultivated yet, limiting the use of host genetic engineering approaches for discovery. On the other hand, genetically tractable hosts such as Escherichia coli, Actinobacteria, and yeast have been developed for the heterologous expression of cyanobacterial BGCs. More recently, there have been increased interests in developing model cyanobacterial strains as heterologous production platforms. Herein, we present recent advances in the heterologous production of cyanobacterial compounds in both cyanobacterial and noncyanobacterial hosts. Emerging strategies for BGC assembly, host engineering, and optimization of BGC expression are included for fostering the broader applications of synthetic biology tools in the discovery of new cyanobacterial natural products. |
format | Online Article Text |
id | pubmed-8210676 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-82106762021-06-17 Heterologous production of cyanobacterial compounds Dhakal, Dipesh Chen, Manyun Luesch, Hendrik Ding, Yousong J Ind Microbiol Biotechnol Natural Products Cyanobacteria produce a plethora of compounds with unique chemical structures and diverse biological activities. Importantly, the increasing availability of cyanobacterial genome sequences and the rapid development of bioinformatics tools have unraveled the tremendous potential of cyanobacteria in producing new natural products. However, the discovery of these compounds based on cyanobacterial genomes has progressed slowly as the majority of their corresponding biosynthetic gene clusters (BGCs) are silent. In addition, cyanobacterial strains are often slow-growing, difficult for genetic engineering, or cannot be cultivated yet, limiting the use of host genetic engineering approaches for discovery. On the other hand, genetically tractable hosts such as Escherichia coli, Actinobacteria, and yeast have been developed for the heterologous expression of cyanobacterial BGCs. More recently, there have been increased interests in developing model cyanobacterial strains as heterologous production platforms. Herein, we present recent advances in the heterologous production of cyanobacterial compounds in both cyanobacterial and noncyanobacterial hosts. Emerging strategies for BGC assembly, host engineering, and optimization of BGC expression are included for fostering the broader applications of synthetic biology tools in the discovery of new cyanobacterial natural products. Oxford University Press 2021-01-25 /pmc/articles/PMC8210676/ /pubmed/33928376 http://dx.doi.org/10.1093/jimb/kuab003 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Natural Products Dhakal, Dipesh Chen, Manyun Luesch, Hendrik Ding, Yousong Heterologous production of cyanobacterial compounds |
title | Heterologous production of cyanobacterial compounds |
title_full | Heterologous production of cyanobacterial compounds |
title_fullStr | Heterologous production of cyanobacterial compounds |
title_full_unstemmed | Heterologous production of cyanobacterial compounds |
title_short | Heterologous production of cyanobacterial compounds |
title_sort | heterologous production of cyanobacterial compounds |
topic | Natural Products |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8210676/ https://www.ncbi.nlm.nih.gov/pubmed/33928376 http://dx.doi.org/10.1093/jimb/kuab003 |
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