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The bacterial microbiome and metabolome in caries progression and arrest
Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reaso...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211139/ https://www.ncbi.nlm.nih.gov/pubmed/34188775 http://dx.doi.org/10.1080/20002297.2021.1886748 |
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author | da Costa Rosa, Thamirys de Almeida Neves, Aline Azcarate-Peril, M. Andrea Divaris, Kimon Wu, Di Cho, Hunyong Moss, Kevin Paster, Bruce J. Chen, Tsute B. Freitas-Fernandes, Liana Fidalgo, Tatiana K. S. Tadeu Lopes, Ricardo Valente, Ana Paula R. Arnold, Roland de Aguiar Ribeiro, Apoena |
author_facet | da Costa Rosa, Thamirys de Almeida Neves, Aline Azcarate-Peril, M. Andrea Divaris, Kimon Wu, Di Cho, Hunyong Moss, Kevin Paster, Bruce J. Chen, Tsute B. Freitas-Fernandes, Liana Fidalgo, Tatiana K. S. Tadeu Lopes, Ricardo Valente, Ana Paula R. Arnold, Roland de Aguiar Ribeiro, Apoena |
author_sort | da Costa Rosa, Thamirys |
collection | PubMed |
description | Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reasons. The experimental model included placement of a modified orthodontic band on smooth surfaces and a mesh on occlusal surfaces. We applied the caries-inducing protocol for 4- and 6-weeks, and subsequently promoted caries lesion arrest via a 2-week toothbrushing period. Lesions were verified clinically and quantitated via micro-CT enamel density measurements. The biofilm microbial composition was determined via 16S rRNA gene Illumina sequencing and NMR spectrometry was used for metabolomics. Results: Biofilm maturation and caries lesion progression were characterized by an increase in Gram-negative anaerobes, including Veillonella and Prevotella. Streptococcus was associated caries lesion progression, while a more equal distribution of Streptococcus, Bifidobacterium, Atopobium, Prevotella, Veillonella, and Saccharibacteria (TM7) characterized arrest. Lactate, acetate, pyruvate, alanine, valine, and sugars were more abundant in mature biofilms compared to newly formed biofilms. Conclusions: These longitudinal bacterial microbiome and metabolome results provide novel mechanistic insights into the role of the biofilm in caries progression and arrest and offer promising candidate biomarkers for validation in future studies. |
format | Online Article Text |
id | pubmed-8211139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-82111392021-06-28 The bacterial microbiome and metabolome in caries progression and arrest da Costa Rosa, Thamirys de Almeida Neves, Aline Azcarate-Peril, M. Andrea Divaris, Kimon Wu, Di Cho, Hunyong Moss, Kevin Paster, Bruce J. Chen, Tsute B. Freitas-Fernandes, Liana Fidalgo, Tatiana K. S. Tadeu Lopes, Ricardo Valente, Ana Paula R. Arnold, Roland de Aguiar Ribeiro, Apoena J Oral Microbiol Original Article Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reasons. The experimental model included placement of a modified orthodontic band on smooth surfaces and a mesh on occlusal surfaces. We applied the caries-inducing protocol for 4- and 6-weeks, and subsequently promoted caries lesion arrest via a 2-week toothbrushing period. Lesions were verified clinically and quantitated via micro-CT enamel density measurements. The biofilm microbial composition was determined via 16S rRNA gene Illumina sequencing and NMR spectrometry was used for metabolomics. Results: Biofilm maturation and caries lesion progression were characterized by an increase in Gram-negative anaerobes, including Veillonella and Prevotella. Streptococcus was associated caries lesion progression, while a more equal distribution of Streptococcus, Bifidobacterium, Atopobium, Prevotella, Veillonella, and Saccharibacteria (TM7) characterized arrest. Lactate, acetate, pyruvate, alanine, valine, and sugars were more abundant in mature biofilms compared to newly formed biofilms. Conclusions: These longitudinal bacterial microbiome and metabolome results provide novel mechanistic insights into the role of the biofilm in caries progression and arrest and offer promising candidate biomarkers for validation in future studies. Taylor & Francis 2021-06-16 /pmc/articles/PMC8211139/ /pubmed/34188775 http://dx.doi.org/10.1080/20002297.2021.1886748 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article da Costa Rosa, Thamirys de Almeida Neves, Aline Azcarate-Peril, M. Andrea Divaris, Kimon Wu, Di Cho, Hunyong Moss, Kevin Paster, Bruce J. Chen, Tsute B. Freitas-Fernandes, Liana Fidalgo, Tatiana K. S. Tadeu Lopes, Ricardo Valente, Ana Paula R. Arnold, Roland de Aguiar Ribeiro, Apoena The bacterial microbiome and metabolome in caries progression and arrest |
title | The bacterial microbiome and metabolome in caries progression and arrest |
title_full | The bacterial microbiome and metabolome in caries progression and arrest |
title_fullStr | The bacterial microbiome and metabolome in caries progression and arrest |
title_full_unstemmed | The bacterial microbiome and metabolome in caries progression and arrest |
title_short | The bacterial microbiome and metabolome in caries progression and arrest |
title_sort | bacterial microbiome and metabolome in caries progression and arrest |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211139/ https://www.ncbi.nlm.nih.gov/pubmed/34188775 http://dx.doi.org/10.1080/20002297.2021.1886748 |
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