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The bacterial microbiome and metabolome in caries progression and arrest

Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reaso...

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Autores principales: da Costa Rosa, Thamirys, de Almeida Neves, Aline, Azcarate-Peril, M. Andrea, Divaris, Kimon, Wu, Di, Cho, Hunyong, Moss, Kevin, Paster, Bruce J., Chen, Tsute, B. Freitas-Fernandes, Liana, Fidalgo, Tatiana K. S., Tadeu Lopes, Ricardo, Valente, Ana Paula, R. Arnold, Roland, de Aguiar Ribeiro, Apoena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211139/
https://www.ncbi.nlm.nih.gov/pubmed/34188775
http://dx.doi.org/10.1080/20002297.2021.1886748
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author da Costa Rosa, Thamirys
de Almeida Neves, Aline
Azcarate-Peril, M. Andrea
Divaris, Kimon
Wu, Di
Cho, Hunyong
Moss, Kevin
Paster, Bruce J.
Chen, Tsute
B. Freitas-Fernandes, Liana
Fidalgo, Tatiana K. S.
Tadeu Lopes, Ricardo
Valente, Ana Paula
R. Arnold, Roland
de Aguiar Ribeiro, Apoena
author_facet da Costa Rosa, Thamirys
de Almeida Neves, Aline
Azcarate-Peril, M. Andrea
Divaris, Kimon
Wu, Di
Cho, Hunyong
Moss, Kevin
Paster, Bruce J.
Chen, Tsute
B. Freitas-Fernandes, Liana
Fidalgo, Tatiana K. S.
Tadeu Lopes, Ricardo
Valente, Ana Paula
R. Arnold, Roland
de Aguiar Ribeiro, Apoena
author_sort da Costa Rosa, Thamirys
collection PubMed
description Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reasons. The experimental model included placement of a modified orthodontic band on smooth surfaces and a mesh on occlusal surfaces. We applied the caries-inducing protocol for 4- and 6-weeks, and subsequently promoted caries lesion arrest via a 2-week toothbrushing period. Lesions were verified clinically and quantitated via micro-CT enamel density measurements. The biofilm microbial composition was determined via 16S rRNA gene Illumina sequencing and NMR spectrometry was used for metabolomics. Results: Biofilm maturation and caries lesion progression were characterized by an increase in Gram-negative anaerobes, including Veillonella and Prevotella. Streptococcus was associated caries lesion progression, while a more equal distribution of Streptococcus, Bifidobacterium, Atopobium, Prevotella, Veillonella, and Saccharibacteria (TM7) characterized arrest. Lactate, acetate, pyruvate, alanine, valine, and sugars were more abundant in mature biofilms compared to newly formed biofilms. Conclusions: These longitudinal bacterial microbiome and metabolome results provide novel mechanistic insights into the role of the biofilm in caries progression and arrest and offer promising candidate biomarkers for validation in future studies.
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spelling pubmed-82111392021-06-28 The bacterial microbiome and metabolome in caries progression and arrest da Costa Rosa, Thamirys de Almeida Neves, Aline Azcarate-Peril, M. Andrea Divaris, Kimon Wu, Di Cho, Hunyong Moss, Kevin Paster, Bruce J. Chen, Tsute B. Freitas-Fernandes, Liana Fidalgo, Tatiana K. S. Tadeu Lopes, Ricardo Valente, Ana Paula R. Arnold, Roland de Aguiar Ribeiro, Apoena J Oral Microbiol Original Article Aim: This in vivo experimental study investigated bacterial microbiome and metabolome longitudinal changes associated with enamel caries lesion progression and arrest. Methods: We induced natural caries activity in three caries-free volunteers prior to four premolar extractions for orthodontic reasons. The experimental model included placement of a modified orthodontic band on smooth surfaces and a mesh on occlusal surfaces. We applied the caries-inducing protocol for 4- and 6-weeks, and subsequently promoted caries lesion arrest via a 2-week toothbrushing period. Lesions were verified clinically and quantitated via micro-CT enamel density measurements. The biofilm microbial composition was determined via 16S rRNA gene Illumina sequencing and NMR spectrometry was used for metabolomics. Results: Biofilm maturation and caries lesion progression were characterized by an increase in Gram-negative anaerobes, including Veillonella and Prevotella. Streptococcus was associated caries lesion progression, while a more equal distribution of Streptococcus, Bifidobacterium, Atopobium, Prevotella, Veillonella, and Saccharibacteria (TM7) characterized arrest. Lactate, acetate, pyruvate, alanine, valine, and sugars were more abundant in mature biofilms compared to newly formed biofilms. Conclusions: These longitudinal bacterial microbiome and metabolome results provide novel mechanistic insights into the role of the biofilm in caries progression and arrest and offer promising candidate biomarkers for validation in future studies. Taylor & Francis 2021-06-16 /pmc/articles/PMC8211139/ /pubmed/34188775 http://dx.doi.org/10.1080/20002297.2021.1886748 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
da Costa Rosa, Thamirys
de Almeida Neves, Aline
Azcarate-Peril, M. Andrea
Divaris, Kimon
Wu, Di
Cho, Hunyong
Moss, Kevin
Paster, Bruce J.
Chen, Tsute
B. Freitas-Fernandes, Liana
Fidalgo, Tatiana K. S.
Tadeu Lopes, Ricardo
Valente, Ana Paula
R. Arnold, Roland
de Aguiar Ribeiro, Apoena
The bacterial microbiome and metabolome in caries progression and arrest
title The bacterial microbiome and metabolome in caries progression and arrest
title_full The bacterial microbiome and metabolome in caries progression and arrest
title_fullStr The bacterial microbiome and metabolome in caries progression and arrest
title_full_unstemmed The bacterial microbiome and metabolome in caries progression and arrest
title_short The bacterial microbiome and metabolome in caries progression and arrest
title_sort bacterial microbiome and metabolome in caries progression and arrest
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211139/
https://www.ncbi.nlm.nih.gov/pubmed/34188775
http://dx.doi.org/10.1080/20002297.2021.1886748
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