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Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats

Neuropathy is a common complication of diabetes affecting a large number of people worldwide. Triphala churna is a formulation mentioned in Ayurveda-a traditional system of medicine. It is a simple powder formulation consisting of powders of three fruits, Emblica officinalis L., Terminalia bellirica...

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Autores principales: Suryavanshi, Sachin V., Barve, Kalyani, Addepalli, Veeranjaneyulu, Utpat, Sachin V., Kulkarni, Yogesh A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211421/
https://www.ncbi.nlm.nih.gov/pubmed/34149415
http://dx.doi.org/10.3389/fphar.2021.662000
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author Suryavanshi, Sachin V.
Barve, Kalyani
Addepalli, Veeranjaneyulu
Utpat, Sachin V.
Kulkarni, Yogesh A.
author_facet Suryavanshi, Sachin V.
Barve, Kalyani
Addepalli, Veeranjaneyulu
Utpat, Sachin V.
Kulkarni, Yogesh A.
author_sort Suryavanshi, Sachin V.
collection PubMed
description Neuropathy is a common complication of diabetes affecting a large number of people worldwide. Triphala churna is a formulation mentioned in Ayurveda-a traditional system of medicine. It is a simple powder formulation consisting of powders of three fruits, Emblica officinalis L., Terminalia bellirica (Gaertn.) Roxb. and Terminalia chebula Retz. Individual components of Triphala churna have anti-diabetic and antioxidant activities. Hence, this study was designed to evaluate the effect of Triphala churna on diabetic neuropathy. Diabetes was induced with streptozotocin (STZ, 55 mg/kg, i. p.) in rats. Animals were grouped and treated orally with Triphala churna at a dose of 250, 500, and 1,000 mg/kg after 6 weeks of diabetes induction for the next 4 weeks. At the end of study, parameters such as body weight, plasma glucose level, motor nerve conduction velocity were determined. The effect of Triphala churna on thermal hyperalgesia, mechanical hyperalgesia, and mechanical allodynia was also determined at the end of study. The plasma cytokine levels like TGF-β1, TNF-α, and IL-1β were determined by ELISA assay. Histopathology study of the sciatic nerve was studied. Western blotting was performed to study the expression of neuronal growth factor.Treatment with Triphala churna showed a significant reduction in plasma glucose and a significant rise in body weight. Triphala treatment significantly increased the motor nerve conduction velocity and decreased the thermal and mechanical hyperalgesia, as well as mechanical allodynia. The treatment significantly inhibited levels of circulatory cytokines like TGF-β1, TNF-α, and IL-1β. Histopathology study confirmed the neuroprotective effect of Triphala churna. The expression of NGF was significantly increased in sciatic nerves after treatment with Triphala churna. From the results, it can be concluded that Triphala churna delays the progression of neuropathy in diabetic rats.
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spelling pubmed-82114212021-06-18 Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats Suryavanshi, Sachin V. Barve, Kalyani Addepalli, Veeranjaneyulu Utpat, Sachin V. Kulkarni, Yogesh A. Front Pharmacol Pharmacology Neuropathy is a common complication of diabetes affecting a large number of people worldwide. Triphala churna is a formulation mentioned in Ayurveda-a traditional system of medicine. It is a simple powder formulation consisting of powders of three fruits, Emblica officinalis L., Terminalia bellirica (Gaertn.) Roxb. and Terminalia chebula Retz. Individual components of Triphala churna have anti-diabetic and antioxidant activities. Hence, this study was designed to evaluate the effect of Triphala churna on diabetic neuropathy. Diabetes was induced with streptozotocin (STZ, 55 mg/kg, i. p.) in rats. Animals were grouped and treated orally with Triphala churna at a dose of 250, 500, and 1,000 mg/kg after 6 weeks of diabetes induction for the next 4 weeks. At the end of study, parameters such as body weight, plasma glucose level, motor nerve conduction velocity were determined. The effect of Triphala churna on thermal hyperalgesia, mechanical hyperalgesia, and mechanical allodynia was also determined at the end of study. The plasma cytokine levels like TGF-β1, TNF-α, and IL-1β were determined by ELISA assay. Histopathology study of the sciatic nerve was studied. Western blotting was performed to study the expression of neuronal growth factor.Treatment with Triphala churna showed a significant reduction in plasma glucose and a significant rise in body weight. Triphala treatment significantly increased the motor nerve conduction velocity and decreased the thermal and mechanical hyperalgesia, as well as mechanical allodynia. The treatment significantly inhibited levels of circulatory cytokines like TGF-β1, TNF-α, and IL-1β. Histopathology study confirmed the neuroprotective effect of Triphala churna. The expression of NGF was significantly increased in sciatic nerves after treatment with Triphala churna. From the results, it can be concluded that Triphala churna delays the progression of neuropathy in diabetic rats. Frontiers Media S.A. 2021-06-03 /pmc/articles/PMC8211421/ /pubmed/34149415 http://dx.doi.org/10.3389/fphar.2021.662000 Text en Copyright © 2021 Suryavanshi, Barve, Addepalli, Utpat and Kulkarni. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Suryavanshi, Sachin V.
Barve, Kalyani
Addepalli, Veeranjaneyulu
Utpat, Sachin V.
Kulkarni, Yogesh A.
Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats
title Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats
title_full Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats
title_fullStr Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats
title_full_unstemmed Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats
title_short Triphala Churna—A Traditional Formulation in Ayurveda Mitigates Diabetic Neuropathy in Rats
title_sort triphala churna—a traditional formulation in ayurveda mitigates diabetic neuropathy in rats
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211421/
https://www.ncbi.nlm.nih.gov/pubmed/34149415
http://dx.doi.org/10.3389/fphar.2021.662000
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