A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells

An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin clea...

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Autores principales: Funnell, Simon G. P., Afrough, Babak, Baczenas, John James, Berry, Neil, Bewley, Kevin R., Bradford, Rebecca, Florence, Clint, Duff, Yann Le, Lewis, Mark, Moriarty, Ryan V., Connor, Shelby L. O., Osman, Karen L., Pullan, Steven, Rashid, Sujatha, Richards, Kevin S., Stemple, Kimberly J., Knezevic, Ivana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211640/
https://www.ncbi.nlm.nih.gov/pubmed/34140522
http://dx.doi.org/10.1038/s41541-021-00346-z
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author Funnell, Simon G. P.
Afrough, Babak
Baczenas, John James
Berry, Neil
Bewley, Kevin R.
Bradford, Rebecca
Florence, Clint
Duff, Yann Le
Lewis, Mark
Moriarty, Ryan V.
Connor, Shelby L. O.
Osman, Karen L.
Pullan, Steven
Rashid, Sujatha
Richards, Kevin S.
Stemple, Kimberly J.
Knezevic, Ivana
author_facet Funnell, Simon G. P.
Afrough, Babak
Baczenas, John James
Berry, Neil
Bewley, Kevin R.
Bradford, Rebecca
Florence, Clint
Duff, Yann Le
Lewis, Mark
Moriarty, Ryan V.
Connor, Shelby L. O.
Osman, Karen L.
Pullan, Steven
Rashid, Sujatha
Richards, Kevin S.
Stemple, Kimberly J.
Knezevic, Ivana
author_sort Funnell, Simon G. P.
collection PubMed
description An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells.
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spelling pubmed-82116402021-07-01 A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells Funnell, Simon G. P. Afrough, Babak Baczenas, John James Berry, Neil Bewley, Kevin R. Bradford, Rebecca Florence, Clint Duff, Yann Le Lewis, Mark Moriarty, Ryan V. Connor, Shelby L. O. Osman, Karen L. Pullan, Steven Rashid, Sujatha Richards, Kevin S. Stemple, Kimberly J. Knezevic, Ivana NPJ Vaccines Article An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells. Nature Publishing Group UK 2021-06-17 /pmc/articles/PMC8211640/ /pubmed/34140522 http://dx.doi.org/10.1038/s41541-021-00346-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Funnell, Simon G. P.
Afrough, Babak
Baczenas, John James
Berry, Neil
Bewley, Kevin R.
Bradford, Rebecca
Florence, Clint
Duff, Yann Le
Lewis, Mark
Moriarty, Ryan V.
Connor, Shelby L. O.
Osman, Karen L.
Pullan, Steven
Rashid, Sujatha
Richards, Kevin S.
Stemple, Kimberly J.
Knezevic, Ivana
A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
title A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
title_full A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
title_fullStr A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
title_full_unstemmed A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
title_short A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells
title_sort cautionary perspective regarding the isolation and serial propagation of sars-cov-2 in vero cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211640/
https://www.ncbi.nlm.nih.gov/pubmed/34140522
http://dx.doi.org/10.1038/s41541-021-00346-z
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